Seven species of red algae from the waters of eastern Long Island, New York were analyzed for their sterol content. The species studied were: Agardhiella tenera, Ahnfeltia plicata, Chondrus crispus, Corallina officinalis^Cystoclonium purpureum var. cirrhosum, Gracilaria foliifera, und Palmaria palmata. The major sterols found belong to C 27 compounds. Cholesterol was the predominant sterol in all species with the possible exception of Palmaria palmata. The presence of cholest-7-en-3/3-ol is reported for the first time in the Order Gigartinales. Considerable variations in the total sterol contents among these species were observed, ranging from l mg/kg for Palmaria palmata to 117 mg/kg for Agardhiella tenera. Comparison of the total sterol content of Palmaria palmata with previous analyses (50 to 190 mg/kg) from waters off eastern Canada indicate that significant regional differences in the sterol content of a given species can occur.