Chemistry of fly ash and cyclone ash leachate from waste materials and effects of ash leachates on bacterial growth, nitrogen-transformation activity, and metal accumulation

Takeuchi, Mio; Kawahata, Hodaka; Gupta, Lallan P.; Itouga, Misao; Sakakibara, Hitoshi; Ohta, Hidekazu; Komai, Takeshi

doi:10.1016/j.jhazmat.2008.10.089

Cited by 8 publications

(3 citation statements)

References 30 publications

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“…Similar to previous findings, the highest maximal specific growth rate was also found for ash. This observation agrees with other findings described in the literature, where it was reported that ashes could enhance Bacillus subtilis growth [ 25 ].…”

Section: Resultssupporting

confidence: 94%

Phosphorus Solubilization by Bacillus Species

2018

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Microbial solubilization applies the natural ability of a microorganism to liberate phosphorus from unavailable structures. The main mechanism recognized to be responsible for the solubilization of phosphorus is the production of different types of organic acids. Three kinds of Bacillus species and three types of raw materials (poultry bones, fish bones, and ash) were tested for solubilization. The following parameters were compared for all discussed cases: pH, specific growth rate, solubilization factor, released phosphorus concentration, and total and individual concentration of organic acids. Utilization of ash brought about the highest specific and maximum specific growth rates. A decrease in pH was observed in most of the discussed cases with the exception of fish bones. At the same time, fish bones had the highest concentration of released P2O5 and the highest total concentration of produced organic acids (gluconic, lactic, acetic, succinic, and propionic) in all discussed cases. The tested Bacillus species produced the mentioned acids with the exception of B. megaterium, where propionic acid was not present. The lactic and acetic acids were those produced in the highest amount. The kind of raw materials and type of Bacillus species used in solubilization had a strong influence on the kind of organic acids that were detected in the broth culture and its total concentration, which had a direct influence on the amount of released phosphorus. The combination of Bacillus megaterium with the fish bones at 5 g/L is proposed as the pair that gives the highest concentration of released phosphorus (483 ± 5 mg/L).

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Section: Resultssupporting

confidence: 94%

Phosphorus Solubilization by Bacillus Species

2018

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“…This increase in the use of CFB technology has resulted in an urgent need to evaluate the ash derived from CFB boilers. However, most studies focus on ash derived from grate-fired boilers [11][12][13][14][15]. To date, few studies address the environmental concerns over biomass ash derived from CFB boilers [16].…”

Section: Introductionmentioning

confidence: 99%

Heavy metal characterization of circulating fluidized bed derived biomass ash

Bai

et al. 2012

Journal of Hazardous Materials

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“…An aliquot (10 mL) of the rearing water in each tank was sampled in the morning and evening and filtered using a 0.2 µm filter. Concentrations of ammonium, nitrate, and nitrite in the rearing water were measured as previously described ( 56 ).…”

Section: Methodsmentioning

confidence: 99%

Fecal metagenomic and metabolomic analyses reveal non-invasive biomarkers of Flavobacterium psychrophilum infection in ayu ( Plecoglossus altivelis )

Takeuchi,

Fujiwara-Nagata,

Kuroda

et al. 2024

mSphere

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With the rapid growth of inland aquaculture worldwide, side effects such as the discharge of nutrients and antibiotics pose a threat to the global environments. A sustainable future for aquaculture requires an effective management system, including the early detection of disease through the monitoring of specific biomarkers in aquaculture tanks. To this end, we investigated whether fish feces in aquaculture tanks could be used for non-invasive health monitoring using ayu ( Plecoglossus altivelis ) infected with Flavobacterium psychrophilum , which causes bacterial cold-water disease worldwide. Feces that were subsequently produced in the tanks were used for metagenomic and metabolomic analyses. The relative abundances of the genera Cypionkella (0.6% ± 1.0%, 0.1% ± 0.2%), Klebsiella (11.2% ± 10.0%, 6.2% ± 5.9%), and F. psychrophilum (0.5% ± 1.0%, 0.0% ± 0.0%) were significantly higher in the feces of the infection challenge test tanks than in those of the control tanks. The abundances of cortisol, glucose, and acetate in the feces of the infection challenge test tanks were 2.4, 2.4, and 1.3 times higher, respectively, than those of the control tanks. Metagenome analysis suggested that acetate was produced by microbes such as Cypionkella . The abundances of indicated microbes or metabolites increased after day 4 of infection at the earliest, and were thus considered possible biomarkers. Our results suggest that feces produced in aquaculture tanks can potentially be used for non-invasive and holistic monitoring of fish diseases in aquaculture systems. IMPORTANCE The aquaculture industry is rapidly growing, yet sustainability remains a challenge. One crucial task is to reduce losses due to diseases. Monitoring fish health and detecting diseases early are key to establishing sustainable aquaculture. Using metagenomic and metabolomic analyses, we found that feces of ayu infected with Flavobacterium psychrophilum contain various specific biomarkers that increased 4 days post-challenge, at the earliest. Our findings are the first step in establishing a novel, non-invasive, and holistic monitoring method for fish diseases in aquaculture systems, especially in ayu, which is an important freshwater fish species in Asia, promoting a sustainable future.

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Chemistry of fly ash and cyclone ash leachate from waste materials and effects of ash leachates on bacterial growth, nitrogen-transformation activity, and metal accumulation

Cited by 8 publications

References 30 publications

Phosphorus Solubilization by Bacillus Species

Phosphorus Solubilization by Bacillus Species

Heavy metal characterization of circulating fluidized bed derived biomass ash

Fecal metagenomic and metabolomic analyses reveal non-invasive biomarkers of Flavobacterium psychrophilum infection in ayu ( Plecoglossus altivelis )

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