Chemistry in Line

Jh, Morris

doi:10.1038/223453a0

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This “tilted” structure of the resulting 2D signals allows for reconstructing a pure shift spectrum by performing a 45° projection of the 2D J ‐resolved spectrum to fully remove the contribution of scalar couplings from the lineshape of the resulting 1D pure shift 1 H spectrum. [ 30,31 ] It should be added that because signals are phase modulated in the indirect dimension, the lineshape is phase twisted after Fourier transformation. For this reason, the resulting J ‐resolved 2D map is in general displayed in magnitude mode, which leads in fine to homonuclear decoupled spectra with broader peaks when the projection is done.…”

Section: Pure Shift Nmr: Decades Of Methodological Developments!mentioning

confidence: 99%

Present and future of pure shift NMR in metabolomics

Chen

Bertho

Caradeuc

et al. 2023

Magnetic Reson in Chemistry

View full text Add to dashboard Cite

NMR is one of the most powerful techniques for the analysis of biological samples in the field of metabolomics. However, the high complexity of fluids, tissues, or other biological materials taken from living organisms is still a challenge for state‐of‐the‐art pulse sequences, thereby limiting the detection, the identification, and the quantification of metabolites. In this context, the resolution enhancement provided by broadband homonuclear decoupling methods, which allows for simplifying 1H multiplet patterns into singlets, has placed this so‐called pure shift technique as a promising approach to perform metabolic profiling with unparalleled level of detail. In recent years, the many advances achieved in the design of pure shift experiments has paved the way to the analysis of a wide range of biological samples with ultra‐high resolution. This review leads the reader from the early days of the main pure shift methods that have been successfully developed over the last decades to address complex samples, to the most recent and promising applications of pure shift NMR to the field of NMR‐based metabolomics.

show abstract

Section: Pure Shift Nmr: Decades Of Methodological Developments!mentioning

confidence: 99%