Chemistry and biology of the alginate of mucoid strains of pseudomonas aeruginosa in cystic fibrosis

Russell, Nicholas J.; Gacesa, Peter

doi:10.1016/0098-2997(88)90002-7

Cited by 72 publications

(48 citation statements)

References 156 publications

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“…This extracellular matrix of glycocalyx in the form of exopolysaccharide of biofilms of P. aeruginosa is mainly composed of alginate. Alginate is an acetylated polymer of beta-D-mannouronic acid and alpha-L-guluronic acids where two uronic acids are arranged in different ways to form block structures [39][40]. Interestingly, in the present study, in biofilm cells a fall in the quantity of alginate was observed 5 th day onwards.…”

Section: Discussionsupporting

confidence: 54%

A Time Course Study of Production of Virulence Factors by Biofilms of Pseudomonas aeruginosa

Mittal¹,

Sharma²,

Chhibber³

et al. 2009

Am. J. Biomed. Sci.

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Pseudomonas aeruginosa is an epitome of opportunistic pathogens. It is the third most common pathogen associated with hospital acquired catheter associated urinary tract infections (CAUTIs). This pathogen forms biofilms on the surface of indwelling catheters which are resistant to antimicrobial agents as well as to host defense mechanisms leading to chronicity and recurrence of infections. In the present study, elaboration of virulence factors by immature and mature biofilm cells of P. aeruginosa was assessed in vitro. It was observed that with increase in time period of biofilm generation from 1 to 4 day (immature biofilms) there was increase in elaboration of majority of virulence traits. However 5 day old biofilms (mature biofilms) were least producers of all the virulence determinants which continued till 7 th day. Implications of these findings in relation to urinary tract infections caused by biofilms of P. aeruginosa have been discussed.

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Section: Discussionsupporting

confidence: 54%

A Time Course Study of Production of Virulence Factors by Biofilms of Pseudomonas aeruginosa

Mittal¹,

Sharma²,

Chhibber³

et al. 2009

Am. J. Biomed. Sci.

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“…It has not been shown whether they also secrete alginate or another extracellular polysaccharide and, if alginate is produced, whether it has the same structure as that from mucoid isolates from CF patients. If there is a difference between mucoid strains from C F and non-CF patients, this might provide a valuable clue to the control of alginate synthesis which, like the pathway, is poorly understood (Russell and Gacesa, 1988).…”

Section: Introductionmentioning

confidence: 99%

“…Most C F patients become colonised, probably by the nonmucoid form of P. aeruginosa which later becomes mucoid (Govan and Harris, 1986). It is not known what stimulates the bacteria to synthesise alginate (Russell and Gacesa, 1988). Assumptions have been made that some factor specific to the CF condition is responsible and that the presence of mucoid P.…”

Section: Introductionmentioning

confidence: 99%

Isolation of mucoid strains of Pseudomonas aeruginosa from non-cystic-fibrosis patients and characterisation of the structure of their secreted alginate

McAvoy

Newton²,

Paull³

et al. 1989

Journal of Medical Microbiology

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Summary. When the incubation period of primary isolation plates was extended to 48 h, mucoid strains of Pseudomonas aeruginosa were found in specimens from various infected sites in patients who did not have cystic fibrosis. The 17 mucoid isolates were characterised in terms of mucoid type, pyocin type, and their sensitivity or resistance to seven p-lactam and two aminoglycoside antibiotics. The carbohydrate, uronic acid (alginate) and protein content of the water-soluble extracellular material of 15 strains was determined. This material was fractionated by ion-exchange chromatography, and the presence of alginate confirmed by the chemical assay of uronic acids and their quantitation by gas-liquid chromatography. Uronic acids were absent from a nonmucoid revertant of one strain. The strains produced alginate with a high content of mannuronic acid and substituted with O-acetyl groups. By proton nuclear magnetic resonance ('H-nmr) analysis the alginate from three strains was shown to lack polyguluronate blocks in its structure. These properties are also found in the alginate of mucoid P. aeruginosa strains from patients with cystic fibrosis.

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“…For example, the development of the chronic hepatitis B virus carrier state has been reported by Bar-naba et al ( 1 ) to be associated with the presence of HLA class I-restricted cytotoxic T lymphocytes (CTL)' that kill B cells producing virus-specific neutralizing antibodies. Acquisition of persistent pulmonary infection with mucoid strains ofPseudomonas aeruginosa among cystic fibrosis (CF) patients remains the major complication leading to morbidity and death (2,3). Persistence of the pathogen despite an intense immune response to bacterial antigens (4)(5)(6)(7)(8) suggests an immunologic defect specific to mucoid P. aeruginosa cells.…”

Section: Introductionmentioning

confidence: 99%

Immune complexes from immunized mice and infected cystic fibrosis patients mediate murine and human T cell killing of hybridomas producing protective, opsonic antibody to Pseudomonas aeruginosa.

et al. 1993

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We examined the basis for the absence in cystic fibrosis (CF) patients of opsonic antibodies to the mucoid exopolysaccharide (MEP) antigen surrounding Pseudomonas aeruginosa that infect these patients. Opsonic antibodies to MEP are found in sera of the minority of CF patients that remain noncolonized into the second to fourth decades of life and protect rodents from chronic P. aeruginosa endobronchial infections. High titers of nonopsonic antibodies to MEP are found in P. aeruginosa-infected CF patients. Immunization of mice with doses of MEP that provoke only nonopsonic antibodies elicited CD3 +, CD8 +, T cell receptor a# receptor +, major histocompatibility complex-unrestricted cytotoxic lymphocytes specific for hybridoma cells producing opsonic but not nonopsonic antibodies. Cytotoxicity was dependent on immune complexes on the surface of the T cells. Normal murine T cells could be activated by concanavalin A and sensitized with immune complexes for cytotoxic killing of hybridoma targets. CF patients infected with P. aeruginosa had serum immune complexes that sensitized concanavalin A-activated human T cells to kill murine hybridoma cells producing opsonic but not nonopsonic antibody. These results could explain the absence in infected CF patients of MEP-specific opsonins, an occurrence that accompanies the persistence of this infectious state. (J. Clin. Invest. 1993. 91:1079-1087 Key words: endobronchial infections * Fc receptors -immunosuppression * mucoid exopolysaccharide* phagocytosis.

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Chemistry and biology of the alginate of mucoid strains of pseudomonas aeruginosa in cystic fibrosis

Cited by 72 publications

References 156 publications

A Time Course Study of Production of Virulence Factors by Biofilms of Pseudomonas aeruginosa

A Time Course Study of Production of Virulence Factors by Biofilms of Pseudomonas aeruginosa

Isolation of mucoid strains of Pseudomonas aeruginosa from non-cystic-fibrosis patients and characterisation of the structure of their secreted alginate

Immune complexes from immunized mice and infected cystic fibrosis patients mediate murine and human T cell killing of hybridomas producing protective, opsonic antibody to Pseudomonas aeruginosa.

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