Chemistries for DNA Nanotechnology

Madsen, Mikael; Gothelf, Kurt V.

doi:10.1021/acs.chemrev.8b00570

Cited by 367 publications

(310 citation statements)

References 585 publications

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“…The programmed self‐assembly of oligonucleotides (ONs) through hydrogen bond and π‐stacking interactions has inspired the burgeoning development of elaborate two‐ and three‐dimensional DNA nanostructures during the last decades . With sizes ranging from nano‐ to micrometers, the user‐defined geometry and the facile chemical functionalization of DNA nanostructures offer ideal nanoscale molecular construction tools for peptide and protein engineering with unparalleled spatial accuracy down to the ångström‐scale level with various chemical and biological applications as long term goals .…”

Section: Introductionmentioning

confidence: 99%

Self‐Assembly of DNA–Peptide Supermolecules: Coiled‐Coil Peptide Structures Templated by d‐DNA and l‐DNA Triplexes Exhibit Chirality‐Independent but Orientation‐Dependent Stabilizing Cooperativity

Lou

Boesen

Christensen

et al. 2020

Chemistry A European J

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DNA nanostructures have been designed and used in many differenta pplications.H owever,t he use of nucleic acid scaffolds to promote the self-assembly of artificial protein mimicsi so nly starting to emerge. Herein five coiledcoil peptide structures were templatedb yt he hybridization of a d-DNA triplex or its mirror-image counterpart, an l-DNA triplex.T he self-assembly of the desiredt rimeric structures in solutionw as confirmed by gel electrophoresis and smallangle X-ray scattering,a nd the stabilizing synergyb etween the two domains was found to be chirality-independent but orientation-dependent. This is the first example of using a nucleic acid scaffold of l-DNA to template the formation of artificial protein mimics. The results may advance the emerging POC-basedn anotechnology field by adding two extra dimensions, that is, chirality and polarity,t op rovide innovative molecular tools forr ational design and bottom-up construction of artificial protein mimics, programmable materials and responsive nanodevices.[a] Dr.[a] Thermal denaturation and annealing temperatures (T m and T a values) of POC and ON-reference triple helixes measured at pH 5.5 as an average of three independent melting temperature determinations shownw ith the corresponding standard deviations. The values in brackets are T m and T a values measured for the corresponding underlying duplexes. The experiments werer ecorded at 275 nm in 10 mm acetate buffer( NaOAc/HOAc)c ontaining1 00 mm NaCl. The concentration of the individual duplex components was 1.0 mm while the TFO component was used in 1.5 mm concentration. The peptide moiety is marked in yellowa nd red (l-peptidea sr ight-handed helix, d-peptidea sl eft-handedh elix), the TFO moiety in dark blue (d-nucleotide) or light blue (l-nucleotide),a nd the DNA duplexm oiety in crimson (d-nucleotide) or in orange (l-nucleotide).

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Section: Introductionmentioning

confidence: 99%

Self‐Assembly of DNA–Peptide Supermolecules: Coiled‐Coil Peptide Structures Templated by d‐DNA and l‐DNA Triplexes Exhibit Chirality‐Independent but Orientation‐Dependent Stabilizing Cooperativity

Lou

Boesen

Christensen

et al. 2020

Chemistry A European J

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“…This heterobifunctional crosslinker approach has been widely applied to generate various DNA‐conjugated protein/peptide ligands, such as RGD‐DNA and ephrin‐DNA conjugates. In addition, self‐labeling polypeptides (SLPs)—that is, SNAP‐tags—could be incorporated into receptor proteins through genetic engineering, thus allowing covalent labeling with DNA for DNA‐based regulation (Figure A, iii) . Many other covalent conjugation methods, such as chemoenzymatic ligation mediated by sortase A, the Halo‐tag method, affinity‐guided labeling, and DNA‐templated conjugation, might provide more efficient, site‐selective, and homogenous formation of protein‐DNA conjugates for the fabrication of receptor‐anchoring DNA nanodevices.…”

Section: Dna As a Receptor‐regulating Toolmentioning

confidence: 99%

Engineering Cell‐Surface Receptors with DNA Nanotechnology for Cell Manipulation

et al. 2019

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Cell‐surface receptors play pivotal roles in the regulation of cell fate. Molecular engineering of cell‐surface receptors enables control of cell signaling and manipulation of cell behavior in a user‐defined way. Currently, the development of chemical‐biological approaches for non‐genetic engineering and regulation of membrane receptors is attracting significant interest. Recent research advances in functional nucleic acids and DNA nanotechnology have made it possible to use DNA as a new and promising molecular toolkit for controlling receptor‐mediated signaling and cell fates. In this minireview we summarize the advances in the use of DNA nanotechnology for the spatiotemporal regulation of cell receptors and highlight practical applications in manipulating cell functions including cell adhesion, cell–cell contact, cell migration, and cellular immunity. We also provide a perspective on the potential of and challenges facing DNA‐based receptor engineering in future applications of cell manipulation and cell‐based therapy.

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“…Despite the added experimental complexities, chemicalm odification is ah ighly effective way to render DNA nanostructures with specific responsiveness, which would significantly broaden the applications of dynamic DNA self-assembly. [73] Sleiman and co-workersa chieved ad imerization of cuboid DNA driven by hydrophobic interactions imparted by dendritic alkyl chains conjugated on selected DNA strands. [74] Baumberg and co-workers developed at hermo-responsive DNA origami flexor bearing temperature-sensitive poly(N-isopropylacrylamide) (PNIPAM) modifiers.…”

Section: Category 4: Chemical Modification Of Dna With Responsive Unitsmentioning

confidence: 99%

Stimuli‐Responsive DNA Self‐Assembly: From Principles to Applications

Jin

et al. 2019

Chemistry A European J

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Stimuli‐responsive DNA self‐assembly shares the advantages of both designed stimuli‐responsiveness and the molecular programmability of DNA structures, offering great opportunities for basic and applied research in dynamic DNA nanotechnology. In this minireview, we summarize the most recent progress in this rapidly developing field. The trigger mechanisms of the responsive DNA systems are first divided into six categories, which are then explained with illustrative examples following this classification. Subsequently, proof‐of‐concept applications in terms of biosensing, in vivo pH‐mapping, drug delivery, and therapy are discussed. Finally, we provide some remarks on the challenges and opportunities of this highly promising research direction in DNA nanotechnology.

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Chemistries for DNA Nanotechnology

Cited by 367 publications

References 585 publications

Self‐Assembly of DNA–Peptide Supermolecules: Coiled‐Coil Peptide Structures Templated by d‐DNA and l‐DNA Triplexes Exhibit Chirality‐Independent but Orientation‐Dependent Stabilizing Cooperativity

Self‐Assembly of DNA–Peptide Supermolecules: Coiled‐Coil Peptide Structures Templated by d‐DNA and l‐DNA Triplexes Exhibit Chirality‐Independent but Orientation‐Dependent Stabilizing Cooperativity

Engineering Cell‐Surface Receptors with DNA Nanotechnology for Cell Manipulation

Stimuli‐Responsive DNA Self‐Assembly: From Principles to Applications

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