ChemInform Abstract: Synthesis of Sulfonic Acid Derivatives by Oxidative Deprotection of Thiols Using tert‐Butyl Hypochlorite.

Joyard, Yoann; Papamicaël, Cyril; Bohn, Pierre; Bischoff, Laurent

doi:10.1002/chin.201337071

Cited by 1 publication

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“…Enzymatic assays were conducted with 250 μL reaction buffer (20 mM potassium phosphate, 10 mM NaCl, pH 8), 5 mM cysteine, and 5 mM synthetic sulfoacrylic acid (SI Appendix, Scheme S1). XcbD (0.5 mg) was injected to initiate the reaction (43). Reactions were incubated for 3 h at 30 °C, followed by quench with molecular weight cutoff filtration.…”

Section: Determination Of 3-sulfo Propionyl Cysteine Production From ...mentioning

confidence: 99%

The pathway for coenzyme M biosynthesis in bacteria

Pun

Wise

et al. 2022

Proc. Natl. Acad. Sci. U.S.A.

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Mercaptoethane sulfonate or coenzyme M (CoM) is the smallest known organic cofactor and is most commonly associated with the methane-forming step in all methanogenic archaea but is also associated with the anaerobic oxidation of methane to CO 2 in anaerobic methanotrophic archaea and the oxidation of short-chain alkanes in Syntrophoarchaeum species. It has also been found in a small number of bacteria capable of the metabolism of small organics. Although many of the steps for CoM biosynthesis in methanogenic archaea have been elucidated, a complete pathway for the biosynthesis of CoM in archaea or bacteria has not been reported. Here, we present the complete CoM biosynthesis pathway in bacteria, revealing distinct chemical steps relative to CoM biosynthesis in methanogenic archaea. The existence of different pathways represents a profound instance of convergent evolution. The five-step pathway involves the addition of sulfite, the elimination of phosphate, decarboxylation, thiolation, and the reduction to affect the sequential conversion of phosphoenolpyruvate to CoM. The salient features of the pathway demonstrate reactivities for members of large aspartase/fumarase and pyridoxal 5′-phosphate–dependent enzyme families.

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Section: Determination Of 3-sulfo Propionyl Cysteine Production From ...mentioning

confidence: 99%