ChemInform Abstract: Synthesis of 17β‐Hydroxyandrost‐4‐en‐3‐one‐7α‐(biotinyl‐6‐N‐ hexylamide) (I), a Conjugate Useful for Affinity Chromatography and for Testosterone Immunoassays.

Luppa, Peter B.; Hauck, Sabine; Schwab, Ingrid; Birkmayer, Christian; Hauptmann, Hagen

doi:10.1002/chin.199639218

Cited by 3 publications

(2 citation statements)

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“…Luminol is more efficient in the free state and is used mostly in enzyme-labeled immunoassays. Furthermore, enzyme-labeled immunoassays using enhanced luminol detection have been proposed for the detection and quantification of estrone and testosterone via biotinylated steroid and HRP avidin conjugate [66]. A large number of emerging clinical tests have been developed using LCL, e.g., cytokines (IL-4, IL-5, IL-6, IL-10) [67], vascular endothelial growth factor-C [68], epidermal growth factor, and benzo[a]pyrene-DNA adducts [69].…”

Section: Immunoassaymentioning

confidence: 99%

Luminol-Based Chemiluminescent Signals: Clinical and Non-clinical Application and Future Uses

Khan

Idrees

Moxley

et al. 2014

Appl Biochem Biotechnol

234

181

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Chemiluminescence (CL) is an important method for quantification and analysis of various macromolecules. A wide range of CL agents such as luminol, hydrogen peroxide, fluorescein, dioxetanes and derivatives of oxalate, and acridinium dyes are used according to their biological specificity and utility. This review describes the application of luminol chemiluminescence (LCL) in forensic, biomedical, and clinical sciences. LCL is a very useful detection method due to its selectivity, simplicity, low cost, and high sensitivity. LCL has a dynamic range of applications, including quantification and detection of macro and micromolecules such as proteins, carbohydrates, DNA, and RNA. Luminol-based methods are used in environmental monitoring as biosensors, in the pharmaceutical industry for cellular localization and as biological tracers, and in reporter gene-based assays and several other immunoassays. Here, we also provide information about different compounds that may enhance or inhibit the LCL along with the effect of pH and concentration on LCL. This review covers most of the significant information related to the applications of luminol in different fields.

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Section: Immunoassaymentioning

confidence: 99%

Luminol-Based Chemiluminescent Signals: Clinical and Non-clinical Application and Future Uses

Khan

Idrees

Moxley

et al. 2014

Appl Biochem Biotechnol

234

181

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“…Because of these important clinical applications, the development of sensitive, simple, and specific assay for T is necessitated. Currently, most methods used to detect the T are the chromatography, such as GC-MS, LC-MS, and GCcombustion-isotope ratio MS [5][6][7][8][9][10][11] [18,19], and chemiluminescence (CL) immunoassays [20]. Recently, CE immunoassay has also been developed for the determination of T [21,22].…”

mentioning

confidence: 99%

A sensitive and rapid immunoassay for quantification of testosterone by microchip electrophoresis with enhanced chemiluminescence detection

et al. 2011

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A sensitive and rapid approach to perform testosterone (T) competitive immunoassay by microchip electrophoresis (MCE) with chemiluminescence (CL) detection was developed. The assay is based on the competitive immunoreactions between T and N-(4-aminobutyl)-N-ethylisoluminol-labeled T (ABEI-T) with a limited amount of antibody (Ab), and the rapid electrophoretic separation of an equilibrated mixture of ABEI-T-Ab complex and free ABEI-T, followed by CL detection using horseradish peroxidase-catalyzed ABEI-H(2)O(2) system. Free ABEI-T and the ABEI-T-Ab complex are well separated within 30 s under the assay conditions. The developed method could be used to determine T with good precision and a detection limit lower than 1.0 nM. This method was applied for the quantification of T in human serum. The results demonstrated that the current MCE-CL-based competitive immunoassay maybe served as an alternative tool for clinical analysis.

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Analysis of Androgens and Their Derivatives

Gower

2010

Steroid Analysis

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ChemInform Abstract: Synthesis of 17β‐Hydroxyandrost‐4‐en‐3‐one‐7α‐(biotinyl‐6‐N‐ hexylamide) (I), a Conjugate Useful for Affinity Chromatography and for Testosterone Immunoassays.

Cited by 3 publications

References 1 publication

Luminol-Based Chemiluminescent Signals: Clinical and Non-clinical Application and Future Uses

Luminol-Based Chemiluminescent Signals: Clinical and Non-clinical Application and Future Uses

A sensitive and rapid immunoassay for quantification of testosterone by microchip electrophoresis with enhanced chemiluminescence detection

Analysis of Androgens and Their Derivatives

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