The chemical structure of a unique polyenic antibiotic enacyloxin Ha (former name: fr. 2) produced by Frateuria (formerly Gluconobacter) sp. W-315 has been determined by extensive spectroscopic studies, in particular by NMRspectral analysis. It has a novel non-lactonic structure involving 3,4-dihydroxycyclohexanecarboxylic acid with a chlorine-containing polyenic and polyhydroxy acyl side chain attached as an ester to the 3-hydroxyl substituent of the acid. : Scanned at roomtemperature, : scanned in liquid nitrogen.tf In ref3, the name of the antibiotic had been reported as enacyloxin II, which has now been renamed as enacyloxin Ila.