ChemInform Abstract: Structure of a Highly Acidic O‐Specific Polysaccharide of Lipopolysaccharide of Pseudoalteromonas haloplanktis KMM 223 (44‐1) Containing L‐Iduronic Acid and D‐QuiNHb4NHb.

Abstract

Structure of a Highly Acidic O-SpecificPolysaccharide of Lipopolysaccharide of Pseudoalteromonas haloplanktis KMM 223 (44-1) Containing L-Iduronic Acid and D-QuiNHb4NHb. --(HANNIFFY, O. M.; SHASHKOV, A. S.; SENCHENKOVA, S. N.; TOMSHICH, S. V.; KOMANDROVA, N. A.; ROMANENKO, L. A.; KNIREL, Y. A.; SAVAGE, A. V.; Carbohydr. Res. 307 (1998) 3-4, 291-298; Chem. Dep., Univ. Coll., Galway, Ire.; EN)

Structure of the O-polysaccharide of Escherichia coli O61, Another E. coli O-antigen That Contains 5,7-Diacetamido-3,5,7,9-tetradeoxy-l-glycero-D-galacto-non-2-ulosonic (Di-N-acetyl-8-epilegionaminic) Acid

“…Other NMR parameters were set essentially as described. [16] RESULTS AND DISCUSSION LPS was isolated from dried cells of E. coli O61 by the phenol-water procedure. On degradation with dilute acetic acid aiming at splitting off lipid A from the carbohydrate moiety, LPS afforded no polysaccharide but a TS owing to cleavage of the O-polysaccharide chain at an acid-labile glycosidic linkage of 8-epilegionamic acid (8eLeg, see below).…”

Structure of the O-polysaccharide of Escherichia coli O112ab containing l-iduronic acid

“…For identification of iduronic acid, the OPS hydrolysate was subjected to methanolysis with 1 M HCl in MeOH at 80°C for 16 h, products were acetylated and analysed by GLC-MS on a HewlettPackard model 5890 Series II instrument equipped with a SPB-5 capillary column (Supelco) using a temperature gradient of 150-320°C at 5°C min À1 . The authentic samples of IdoA and AltA were derived from the O-polysaccharides of Pseudoalteromonas haloplanktis KMM 223 (44-1) 16 and Proteus mirabilis O10, 28 respectively. The absolute configurations of the monosaccharides were determined by GLC of the acetylated (S)-2-octyl glycosides as described.…”

“…GLC analysis of alditol acetates after full acid hydrolysis of the OPS revealed Glc, GlcN, and GalN in the ratios $1:1:2, respectively. Analysis of uronic acids in the OPS hydrolysate using a sugar analyzer demonstrated an acidic monosaccharide, which has a retention time different from those of glucuronic, mannuronic, galacturonic, and guluronic acids but identical to that of iduronic and altruronic acid, which could not be separated on the Dionex A · 8 anion-exchange resin used (see also published data 16 separated as acetylated methyl esters methyl glycosides, the derivatives from the OPS being identical in GLC-MS to those from iduronic acid and different from the derivatives from altruronic acid. Hence, the OPS contains iduronic acid (IdoA), which was also confirmed by NMR spectroscopy data given below.…”

“…To the best of our knowledge, L L-iduronic acid has been previously found only once in lipopolysaccharides, namely in the O-polysaccharide of a marine bacterium Pseudoalteromonas haloplanktis KMM 223 (44-1). 16 Iduronic acid of unknown absolute configuration has been reported as a component of surface polysaccharides of Clostridium perfringens. 22,23 Based on the data obtained, it was concluded that the OPS of E. coli O112ab has the following structure:…”

Structure of the O-antigen of Yersinia pseudotuberculosis O:4a revised

“…The NMR spectra of the polysaccharide were fully assigned using 2D COSY, TOCSY and 1 H, 13 C HSQC experiments ( Table 1). The spin systems for three mannose residues, designated as Man I -Man III according to their sequence in the repeating unit (see below), were identified by tracing connectivities from signals for H-1 and H-2 in the TOCSY spectrum.…”