ChemInform Abstract: Solid‐State Fermentation

Bhargav, S.; Panda, Bibhu Prasad; Ali, Mazhar; Javed, Saleem

doi:10.1002/chin.200830268

Cited by 13 publications

(13 citation statements)

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“…SSF agro-industrial substrates are considered best for cost effective enzyme production [58]. The isolate GAA 31.1 is competent to utilize preferably all the test substrates and found potato waste as the most ideal for amylase production (326.72 ± 6.081 U/ml), followed by rice bran and gram husk (Fig.…”

Section: Resultsmentioning

confidence: 99%

Identification and product optimization of amylolytic Rhodococcus opacus GAA 31.1 isolated from gut of Gryllotalpa africana

Banerjee

Maiti

Roy

2016

Journal of Genetic Engineering and Biotechnology

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An attempt has been made to isolate potent amylase producing gut bacteria from Gryllotalpa africana. Out of 82 isolates, GAA 31.1 was selected as potent producer, having enzyme activity 9.6 ± 0.861 U/ml. The isolate GAA 31.1 was identified as Rhodococcus opacus following morphological, biochemical, physiological characterization and phylogenetic analysis through 16S rRNA gene sequencing. Fatty acid methyl ester profile of the isolate was also studied. The optimized physical cultural conditions for amylase production were found as incubation period 48 h, inoculum volume 2%, initial pH of the fermentation medium 7.0, temperature 38 °C and aeration at 150 rpm. Optimum nutrient conditions were determined as: supplementation of maltose 1.4% and sodium nitrate 1.4%. Surfactants SDS, EDTA, Tween 80 and Triton X-100 showed positive effect on enzyme production. Riboflavin (50 μg/ml) among the tested vitamins stimulated the production maximally. The isolate was also able to produce amylase using agro-industrial waste. This actinobacterium may be a potent candidate for amylase as it is capable of enhanced production (326.72 ± 6.081 U/ml) by utilizing agro-residues.

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Section: Resultsmentioning

confidence: 99%

Identification and product optimization of amylolytic Rhodococcus opacus GAA 31.1 isolated from gut of Gryllotalpa africana

Banerjee

Maiti

Roy

2016

Journal of Genetic Engineering and Biotechnology

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“…However, this is only applicable to small-scale SSF. Adequate mixing of the substrate with sparged oxygen can also help; it aids in the homogeneity of the bed but also ensures adequate heat and mass transfer [112]. Bioreactors commonly used for SSF can be divided into four types based on the agitation system employed or the type of aeration.…”

Section: Scp Production Involves Several Basic Steps [102]mentioning

confidence: 99%

Biotechnological Production of Sustainable Microbial Proteins from Agro-Industrial Residues and By-Products

Bajić

Vučurović

Vasić

et al. 2022

Foods

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Microbial proteins, i.e., single-cell proteins or microbial biomass, can be cultivated for food and animal feed due to their high protein content and the fact that they represent a rich source of carbohydrates, minerals, fats, vitamins, and amino acids. Another advantage of single-cell proteins is their rapid production due to the growth rate of microorganisms and the possibility of using agro-industrial waste, residues and by-products for production through this renewable technology. Agro-industrial residues and by-products represent materials obtained from various processes in agriculture and agriculture-related industries; taking into account their composition and characteristics, as well as vast amounts, they have an enormous potential to generate sustainable bioproducts, such as microbial proteins. This review aims to summarize contemporary scientific research related to the production of microbial proteins on various agro-industrial residues and by-products, as well as to emphasize the current state of production of single-cell proteins and the importance of their production to ease the food crisis and support sustainable development.

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“…Optimum pH for enzyme production was also carried out using various pH through the protocol described earlier by Bhargav et al (2008).…”

Section: Optimization Of Culture Conditions For Enzyme Productionmentioning

confidence: 99%

Commercial production of alpha amylase enzyme for potential use in the textile industries in Bangladesh

2018

Int. J. Biosci.

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In Bangladesh textile and apparel industry is in the leading position in industrial structure. It contributes 12% of the country's GDP and also accounts for almost 78% of total exports, making it countries leading foreign exchange earner. Alpha amylase is an enzyme used in textile industry for smoothening the fabric. It is also used in many washing powder, food and paper industries. But yet we do not produce this enzyme in Bangladesh though its production and purification technique is rather simple. Each year we spend a huge amount of foreign currency to import it. In this study we scaled down the production cost of alpha amylase in a pilot project.Aspergillus niger was used in this project for alpha amylase production. A. niger was cultured in PDB (Potato Dextrose Broth) medium for seed production. Wheat bran was used as substrate for fermentation. Substrates were pre-treated with 1% NaOH for hydrolysis and washed with dH2O until neutral pH obtained. Substrates were then dried overnight using oven. MSM (Minimum Salt Medium) medium with some modifications was used for fermentation. Fermentation was carried out at 28°C at a pH of 6.2. Extraction of the enzyme was carried out by centrifugation. CMC and DNS assay showed good performance of the extracted enzyme. The calculated production cost of our enzyme is 57.92 taka per liter which is cheaper than imported ones (~450 taka per liter).Therefore, we strongly recommend that entrepreneur should be contacted to go for industrial scale alpha amylase production in Bangladesh.

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ChemInform Abstract: Solid‐State Fermentation

Cited by 13 publications

References 0 publications

Identification and product optimization of amylolytic Rhodococcus opacus GAA 31.1 isolated from gut of Gryllotalpa africana

Identification and product optimization of amylolytic Rhodococcus opacus GAA 31.1 isolated from gut of Gryllotalpa africana

Biotechnological Production of Sustainable Microbial Proteins from Agro-Industrial Residues and By-Products

Commercial production of alpha amylase enzyme for potential use in the textile industries in Bangladesh

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