“…Separation of isomers in DL--tocopherol based on the three chiral centers in the phytyl tail was reported with HPLC using a chiral stationary phase and subsequent capillary GC on an achiral stationary phase [15,16]. Another report also used a chiral stationary phase [17] to separate DL--tocopherol acetate isomers into four peaks (peak area ratio=4:2:1:1) by HPLC using Chiralpak OP(+), where 2(R)-isomers constituted the first peak and 2(S) isomers were separated into three peaks (peak area ratio=2:1:1).…”