ChemInform Abstract: Progress in the Separation of Enantiomers of Chiral Drugs by TLC Without Their Prior Derivatization

Subert, J; Slais, K.

doi:10.1002/chin.200130291

Cited by 2 publications

(2 citation statements)

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“…Therefore, electrokinetic chromatography (EKC) as a type of CE with luminescence detection was used to separate chiral MTX [115]. In addition, amperometric biosensors [119], voltammetric methods [120], potentiometric membrane electrodes [121], TLC [122], HPLC [123], and CE [[124], [125], [126]] were also reported to be used for chiral determination of MTX. The instrumental parameters for CE detection of MTX are described in Table 4.…”

Section: Capillary Electrophoresis (Ce)mentioning

confidence: 99%

Analytical methodologies for determination of methotrexate and its metabolites in pharmaceutical, biological and environmental samples

Karami

Ranjbar

Ghasemi

et al. 2019

Journal of Pharmaceutical Analysis

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Methotrexate (MTX) is a folate antagonist drug used for several diseases, such as cancers, various malignancies, rheumatoid arthritis (RA) and inflammatory bowel disease. Due to its structural features, including the presence of two carboxylic acid groups and its low native fluorescence, there are some challenges to develop analytical methods for its determination. MTX is metabolized to 7-hydroxymethotrexate (7-OH-MTX), 2,4-diamino-N10-methylpteroic acid (DAMPA), and the active MTX polyglutamates (MTXPGs) in the liver, intestine, and red blood cells (RBCs), respectively. Additionally, the drug has a narrow therapeutic range; hence, its therapeutic drug monitoring (TDM) is necessary to regulate the pharmacokinetics of the drug and to decrease the risk of toxicity. Due to environmental toxicity of MTX; its sensitive, fast and low cost determination in workplace environments is of great interest. A large number of methodologies including high performance liquid chromatography equipped with UV–visible, fluorescence, or electrochemical detection, liquid chromatography-mass spectroscopy, capillary electrophoresis, UV–visible spectrophotometry, and electrochemical methods have been developed for the quantitation of MTX and its metabolites in pharmaceutical, biological, and environmental samples. This paper will attempt to review several published methodologies and the instrumental conditions, which have been applied to measure MTX and its metabolites within the last decade.

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Section: Capillary Electrophoresis (Ce)mentioning

confidence: 99%

Analytical methodologies for determination of methotrexate and its metabolites in pharmaceutical, biological and environmental samples

Karami

Ranjbar

Ghasemi

et al. 2019

Journal of Pharmaceutical Analysis

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“…Examination of the literature reveals that chiral discrimination of MTX has been achieved by means of different analytical techniques such as amperometric biosensors [ 31 ], voltammetric methods [ 32 ], potentiometric membrane electrodes [ 1 ], TLC [ 33 ], HPLC [ 6 , 34 , 35 ], and CE [ 5 , 36 – 39 ]. In the case of CE, the chiral separation of MTX and other compounds was carried out to evaluate vancomycin [ 36 ], d -(+)-tubocurarine [ 37 ], and cyclosophoraoses [ 39 ] as chiral selector.…”

Section: Introductionmentioning

confidence: 99%

Quenched phosphorescence as alternative detection mode in the chiral separation of methotrexate by electrokinetic chromatography

et al. 2011

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Quenched phosphorescence was used, for the first time, as detection mode in the chiral separation of methotrexate (MTX) enantiomers by electrokinetic chromatography. The detection is based on dynamic quenching of the strong emission of the phosphorophore 1-bromo-4-naphthalene sulfonic acid (BrNS) by MTX under deoxygenated conditions. The use of a background electrolyte with 3 mg/mL 2-hydroxypropyl-β-cyclodextrin and 20% MeOH in 25 mM phosphate buffer (pH 7.0) and an applied voltage of 30 kV allowed the separation of l-MTX and its enantiomeric impurity d-MTX with sufficient resolution. In the presence of 1 mM BrNS, a detection limit of 3.2 × 10−7 M was achieved, about an order of magnitude better than published techniques based on UV absorption. The potential of the method was demonstrated with a degradation study and an enantiomeric purity assessment of l-MTX. Furthermore, l-MTX was determined in a cell culture extract as a proof-of-principle experiment to show the applicability of the method to biological samples.FigureA quenched phosphorescence method was developed and coupled to chiral EKC for the sensitive detection of the drug L-methotrexate and its impurity D-MTX; unspiked pharmaceutical formulation

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ChemInform Abstract: Progress in the Separation of Enantiomers of Chiral Drugs by TLC Without Their Prior Derivatization

Cited by 2 publications

References 0 publications

Analytical methodologies for determination of methotrexate and its metabolites in pharmaceutical, biological and environmental samples

Analytical methodologies for determination of methotrexate and its metabolites in pharmaceutical, biological and environmental samples

Quenched phosphorescence as alternative detection mode in the chiral separation of methotrexate by electrokinetic chromatography

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