ChemInform Abstract: Phenolic Constituents of Licorice. Part 9. Minor Flavonoids from Licorice.

Hatano, Tsutomu; Aga, Yasuhiro; Shintani, Yasushi; Ito, Hideyuki; Okuda, Takuo; Yoshida, Takashi

doi:10.1002/chin.200110243

Cited by 2 publications

(1 citation statement)

References 1 publication

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“…These pure compounds were then characterized by HRMS and NMR spectra. Based on comparison of the spectral data with the literature values , 24 flavonoids were identified individually. The purity and weight of compounds were listed in Table and the 1 H NMR and 13 C NMR data were illustrated in Supporting Information.…”

Section: Resultsmentioning

confidence: 99%

Purification of flavonoids from licorice using an off-line preparative two-dimensional normal-phase liquid chromatography/reversed-phase liquid chromatography method

Fan

et al. 2016

J. Sep. Science

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An orthogonal (71.9%) off-line preparative two-dimensional normal-phase liquid chromatography/reversed-phase liquid chromatography method coupled with effective sample pretreatment was developed for separation and purification of flavonoids from licorice. Most of the nonflavonoids were firstly removed using a self-made Click TE-Cys (60 μm) solid-phase extraction. In the first dimension, an industrial grade preparative chromatography was employed to purify the crude flavonoids. Click TE-Cys (10 μm) was selected as the stationary phase that provided an excellent separation with high reproducibility. Ethyl acetate/ethanol was selected as the mobile phase owing to their excellent solubility for flavonoids. Flavonoids co-eluted in the first dimension were selected for further purification using reversed-phase liquid chromatography. Multiple compounds could be isolated from one normal-phase fraction and some compounds with bad resolution in one-dimensional liquid chromatography could be prepared in this two-dimensional system owing to the orthogonal separation. Moreover, this two-dimensional liquid chromatography method was beneficial for the preparation of relatively trace flavonoid compounds, which were enriched in the first dimension and further purified in the second dimension. Totally, 24 flavonoid compounds with high purity were obtained. The results demonstrated that the off-line two-dimensional liquid chromatography method was effective for the preparative separation and purification of flavonoids from licorice.

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Section: Resultsmentioning

confidence: 99%

Purification of flavonoids from licorice using an off-line preparative two-dimensional normal-phase liquid chromatography/reversed-phase liquid chromatography method

Fan

et al. 2016

J. Sep. Science

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Dietary Effects of Herbal Mixture on the Laying Performance, Blood Parameters and Immune Response in Laying Hens

Kim¹,

Kang²,

Hwangbo³

et al. 2015

Korean Journal of Poultry Science

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The objective of this experiment was to investigate the effect of dietary supplementation of herbal medication (HM) on laying performance, blood parameter, and immune response in laying hens. A total of 800 Hy-Line Brown laying hens of 60 weeks of age were randomly allotted to one of four dietary treatments with four replicates per treatment. Three additional diets were prepared by adding 0.5, 1.0 or 1.5 % of HM to the basal diet. Feeding trial lasted 7 weeks under 16L:8D lighting regimen. The diet and water were available ad libitum. Hen-day egg production in groups fed diets with 0.5% and 1.5% HM was significantly (P<0.05) higher than that of control. Feed conversion ratio in group fed diet with 0.5% HM showed the lowest value (P<0.05). Egg weight, feed intake, broken and shell-less egg production were not affected HM supplementation levels. Egg yolk color was significantly greater in all HM supplemented groups than basal treatment. However, eggshell strength, eggshell thickness, eggshell color, and Haugh units were not affected by HM supplementation level. There were no differences in leukocyte counts among the treatments. Plamsa IgM concentration was (P<0.05) higher in all HM treated groups. In conclusion, dietary HM improved egg production, and IgM of laying hens.

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ChemInform Abstract: Phenolic Constituents of Licorice. Part 9. Minor Flavonoids from Licorice.

Cited by 2 publications

References 1 publication

Purification of flavonoids from licorice using an off-line preparative two-dimensional normal-phase liquid chromatography/reversed-phase liquid chromatography method

Purification of flavonoids from licorice using an off-line preparative two-dimensional normal-phase liquid chromatography/reversed-phase liquid chromatography method

Dietary Effects of Herbal Mixture on the Laying Performance, Blood Parameters and Immune Response in Laying Hens

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