ChemInform Abstract: Hydrogen‐gas Production with Citrobacter intermedius and Clostridium pasteurianum.

Brosseau, James D.; Zajic, J. E.

doi:10.1002/chin.198237347

Cited by 19 publications

(10 citation statements)

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“…The maximum H 2 yield reached 2.67 mol H 2 per mol carbohydrate added (=2.73 mol H 2 per mol carbohydrate converted) at a temperature of 30°C and an initial pH of 7. The maximum hydrogen yields in the previous studies were 1.0-2.4 mol of H 2 per mol of glucose, which were obtained using a pure culture and a mixed culture [10][11][12][13][14][15]. The value observed in this study was a little higher than those seen in previous studies.…”

Section: Results Of the Biogas Analysiscontrasting

confidence: 63%

Biohydrogen using leachate from an industrial waste landfill as inoculum

Watanabe

Yoshino

2011

J Mater Cycles Waste Manag

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Since hydrogen is a renewable energy source, biohydrogen has been researched in recent years. However, data on hydrogen fermentation by a leachate from a waste landfill as inoculum are scarce. We investigated hydrogen production using a leachate from an industrial waste landfill in Kanagawa Prefecture. The results showed no methane gas production, and the leachate was a suitable inoculum for hydrogen fermentation. The maximum H 2 yield was 2.67 mol of H 2 per mol of carbohydrate added, obtained at 30°C and an initial pH of 7. The acetate and butyrate production was significant when the H 2 yield was higher. Oxidation-reduction potential analysis of the culture suggested that hydrogen-producing bacteria in the leachate were facultative anaerobic. Scanning electron microscope observations revealed that the hydrogen-producing bacteria comprised bacilli about 2 lm in length.

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Section: Results Of the Biogas Analysiscontrasting

confidence: 63%

Biohydrogen using leachate from an industrial waste landfill as inoculum

Watanabe

Yoshino

2011

J Mater Cycles Waste Manag

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“…For example, Lin and Chang (1999) measured H 2 concentrations in the biogas of 43 -53% while Liu and Fang (2002) measured hydrogen concentration of 61 -66% regardless of the HRT. For mixed culture reactors, conversion efficiencies of glucose to hydrogen have been reported of 20.2% (Mizuno et al, 2000), 27.5% (Nakamura et al, 1993), 34% (Brosseau and Zajic, 1982), and 38% (Lin and Chang, 1999). For pure cultures, conversion efficiencies similarly range from 25% for Enterobacter aerogenes (Rachman et al, 1998) to as much as 53% for Clostridium butyricum (Kataoka et al, 1997).…”

Section: Discussionmentioning

confidence: 95%

Biological hydrogen production using a membrane bioreactor

Iyer

Bruns

et al. 2004

Biotech & Bioengineering

184

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A cross-flow membrane was coupled to a chemostat to create an anaerobic membrane bioreactor (MBR) for biological hydrogen production. The reactor was fed glucose (10,000 mg/L) and inoculated with a soil inoculum heat-treated to kill non-spore-forming methanogens. Hydrogen gas was consistently produced at a concentration of 57-60% in the headspace under all conditions. When operated in chemostat mode (no flow through the membrane) at a hydraulic retention time (HRT) of 3.3 h, 90% of the glucose was removed, producing 2200 mg/L of cells and 500 mL/h of biogas. When operated in MBR mode, the solids retention time (SRT) was increased to SRT = 12 h producing a solids concentration in the reactor of 5800 mg/L. This SRT increased the overall glucose utilization (98%), the biogas production rate (640 mL/h), and the conversion efficiency of glucose-to-hydrogen from 22% (no MBR) to 25% (based on a maximum of 4 mol-H(2)/mol-glucose). When the SRT was increased from 5 h to 48 h, glucose utilization (99%) and biomass concentrations (8,800 +/- 600 mg/L) both increased. However, the biogas production decreased (310 +/- 40 mL/h) and the glucose-to-hydrogen conversion efficiency decreased from 37 +/- 4% to 18 +/- 3%. Sustained permeate flows through the membrane were in the range of 57 to 60 L/m(2) h for three different membrane pore sizes (0.3, 0.5, and 0.8 microm). Most (93.7% to 99.3%) of the membrane resistance was due to internal fouling and the reversible cake resistance, and not the membrane itself. Regular backpulsing was essential for maintaining permeate flux through the membrane. Analysis of DNA sequences using ribosomal intergenic spacer analysis indicated bacteria were most closely related to members of Clostridiaceae and Flexibacteraceae, including Clostridium acidisoli CAC237756 (97%), Linmingia china AF481148 (97%), and Cytophaga sp. MDA2507 AF238333 (99%). No PCR amplification of 16s rRNA genes was obtained when archaea-specific primers were used.

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“…However, the reported conversion efficiencies of Clostridium intermedius varies up to 38% from glucose, while that of Clostridium butyricum varies up to 55% from sucrose and that of Clostridium sp. up to 59% from xylose [14,74,166].…”

Section: Hydrogen Production From Organic Substratesmentioning

confidence: 99%

Microbial diversity and genomics in aid of bioenergy

Kalia

Purohit

2008

J Ind Microbiol Biotechnol

104

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In view of the realization that fossil fuels reserves are limited, various options of generating energy are being explored. Biological methods for producing fuels such as ethanol, diesel, hydrogen (H2), methane, etc. have the potential to provide a sustainable energy system for the society. Biological H2 production appears to be the most promising as it is non-polluting and can be produced from water and biological wastes. The major limiting factors are low yields, lack of industrially robust organisms, and high cost of feed. Actually, H2 yields are lower than theoretically possible yields of 4 mol/mol of glucose because of the associated fermentation products such as lactic acid, propionic acid and ethanol. The efficiency of energy production can be improved by screening microbial diversity and easily fermentable feed materials. Biowastes can serve as feed for H2 production through a set of microbial consortia: (1) hydrolytic bacteria, (2) H2 producers (dark fermentative and photosynthetic). The efficiency of the bioconversion process may be enhanced further by the production of value added chemicals such as polydroxyalkanoate and anaerobic digestion. Discovery of enormous microbial diversity and sequencing of a wide range of organisms may enable us to realize genetic variability, identify organisms with natural ability to acquire and transmit genes. Such organisms can be exploited through genome shuffling for transgenic expression and efficient generation of clean fuel and other diverse biotechnological applications.

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ChemInform Abstract: Hydrogen‐gas Production with Citrobacter intermedius and Clostridium pasteurianum.

Cited by 19 publications

References 1 publication

Biohydrogen using leachate from an industrial waste landfill as inoculum

Biohydrogen using leachate from an industrial waste landfill as inoculum

Biological hydrogen production using a membrane bioreactor

Microbial diversity and genomics in aid of bioenergy

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