ChemInform Abstract: Enzyme Mimics: Advances and Applications

Kuah, Evelyn; Toh, Seraphina; Yee, Jessica; Ma, Qian; Gao, Zhiqiang

doi:10.1002/chin.201635205

Cited by 1 publication

(1 citation statement)

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“…[13][14][15][16] Nanozymes comprise many types of materials, iron oxide, vanadium oxide, cerium oxide, manganese oxide, gold, platinum, palladium, gold-silver alloys, carbon nanotubes, graphene, metal chalcogenides, and other [17][18][19][20][21][22][23][24][25][26] Compared with natural enzymes, nanozymes are advantageous in several aspects, such as low cost, ease of mass production, robustness to harsh environments, high stability, long-term storage, large surface area for further modification and bioconjugation and so on. [27][28][29][30][31][32] Previously, we found Au-Pt core/shell nanorods (Au@Pt NRs) have intrinsic multiple enzyme mimetic capability, for example, oxidase, peroxidase, and catalase mimetic activity. [33][34][35][36][37] The as-synthesized Au@Pt NRs are able to catalyze color reactions in immunoassay and, therefore, can be used to replace the enzymes in conventional ELISA.…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of rubella virus using antigen-conjugated Au@Pt nanorods as nanozyme probe

Zhang¹,

Tian²,

Lin³

et al. 2018

IJN

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BackgroundAs nanotechnology advances rapidly, the nanozymes which could replace protein enzymes in bioanalytical assays bring a new opportunity to the development of simple and sensitive diagnostic tools.PurposeAntibody-conjugated Au-Pt core/shell nanorods (Au@Pt NRs) have been used for nanozyme probes for diagnosis of rubella virus. Au@Pt NRs, prepared by Au nanorod-mediated growth, exhibit peroxidase-like activities and could serve as an inexpensive replacement for horseradish peroxidase (HRP) in conjugation of antigen.MethodUsing a capture enzyme-linked immunosorbant assay for the determination of rubella virus.ResultsCompared with antibody-conjugated HRP, the antigen-conjugated Au@Pt NRs are more stable and more robust, but less expensive. Based on the enhanced catalytic properties of this nanozyme probe, it was found that the antigen-conjugated Au@Pt NRs-based enzyme-linked immunosorbant assay exhibited good sensitivity.ConclusionOur results indicate that these antigen-conjugated Au@Pt NRs represent a suitable nanozyme probe for future clinical applications under various conditions.

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