ChemInform Abstract: Antimicrobial Natural Products: An Update on Future Antibiotic Drug Candidates

Saleem, Muhammad; Nazir, Mamona; Ali, Muhammad; Hussain, Hidayat; Lee, Yong Sup; Riaz, Naheed; Jabbar, Abdul

doi:10.1002/chin.201021265

Cited by 118 publications

(155 citation statements)

References 1 publication

Supporting

Mentioning

127

Contrasting

Unclassified

Order By: Relevance

“…Since implied in protection from insects and microbial infections, some secondary metabolites are the natural defense system of plants [22,23] , which explains the antimicrobial potency of those substances. The principally chemical families responsible for antimicrobial activity in medicinal plants are polyphenols, terpenoids, alkaloids, iridoids, saponins, coumarins, and xanthones [7] . The genus Thymus, which belongs to Lamiaceae family, is well known by its high content of terpenoids [10] and polyphenols [24] .…”

Section: Discussionmentioning

confidence: 99%

“…Since it is rich in terpenoids and flavonoids, which can be used as new pharmacologically acceptable antibiotics in the future [7,26] , T. lanceolatus can be a source of new antibiotics. In the other hand, because of the absence of data for the chemical composition, further refined studies on extracts obtained from this plant are interesting.…”

Section: Discussionmentioning

confidence: 99%

“…In modern medicine, the discovery of bioactive molecules from microbial origin and synthetic antimicrobial agents decreased the use of traditional plant preparations, especially in developed countries. But in last decade, and besides their toxicities and side-effects [6] , it has become clear that current antimicrobial drugs are losing their effectiveness [7] , due to the emergence of resistance in pathogens to those antibiotics, which causes therapeutic failures and death of individuals [8] . Therefore, there is a continuing need to search for new antibiotics [3,7] .…”

Section: Introductionmentioning

confidence: 99%

“…But in last decade, and besides their toxicities and side-effects [6] , it has become clear that current antimicrobial drugs are losing their effectiveness [7] , due to the emergence of resistance in pathogens to those antibiotics, which causes therapeutic failures and death of individuals [8] . Therefore, there is a continuing need to search for new antibiotics [3,7] . Since plant-derived antibacterials are always a source of novel therapeutics [9] , many researchers in field of ethnopharmacology have concentrated in studies of those compounds in the past few decades [5] on a promising and wealthy source for safe and effective new antimicrobial agents.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Phytochemical screening and in vitro antimicrobial activity of Thymus lanceolatus Desf. from Algeria

Benbelaïd

Khadir

Abdoune

et al. 2013

Asian Pacific Journal of Tropical Disease

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Phytochemical screening and in vitro antimicrobial activity of Thymus lanceolatus Desf. from Algeria

Benbelaïd

Khadir

Abdoune

et al. 2013

Asian Pacific Journal of Tropical Disease

View full text Add to dashboard Cite

“…They are a structurally diverse class of natural products that show a wide variety of bioactive properties, including anticancer, antifungal, immuno-suppressive and anti-aging activity. 2 Oligomycin A, which was first isolated from a culture of soil bacterium Streptomyces diastatochromogenes in 1954, is a major component of oligomycins as a series of 26-membered macrocyclic lactones ( Figure 1). 3 This antibiotic has also been reported to possess some potential antitumor activity.…”

mentioning

confidence: 99%

Generation of reduced macrolide analogs by regio-specific biotransformation

Park

Han

et al. 2010

J Antibiot

View full text Add to dashboard Cite

The emergence of antibiotic-resistant pathogens has rekindled interest toward the discovery of new antibiotics. Semi-synthetic or biosynthetic approach using microbe as a biocatalyst can be an efficient tool for the targeted modification of existing antibiotic chemical scaffolds to create the next generation of antibiotics as well as previously uncharacterized therapeutic agents. 1 The macrolide antibiotics are a group of polyketides whose activity is derived from the presence of a large macrocyclic lactone ring. They are a structurally diverse class of natural products that show a wide variety of bioactive properties, including anticancer, antifungal, immuno-suppressive and anti-aging activity. 2 Oligomycin A, which was first isolated from a culture of soil bacterium Streptomyces diastatochromogenes in 1954, is a major component of oligomycins as a series of 26-membered macrocyclic lactones (Figure 1). 3 This antibiotic has also been reported to possess some potential antitumor activity. 4 Tilmicosin, a semi-synthetic tylosin that occurs as a 16-membered macrolide in the fermentation of S. fradiae, has use as a veterinary antibiotic (Figure 1). 5 We recently reported the unique and regio-specific hydrogenation activity toward unsaturated 12-(methymycin) and 14-membered (pikromycin) macrolides by S. venezuelae, and its application to the generation of unnatural 16-membered macrolides. 6 This bacterial reduction system could recognize the specific structural elements around the target double bond, that is, a carbonyl functional group on the neighboring carbon and a methyl group onto another adjoining carbon in the polyketide backbone (depicted as faded rectangles in Figure 1).In this study, an attempt was made to expand the applicability of the unique bio-hydrogenation activity of S. venezuelae toward other unsaturated macrolide antibiotics (natural oligomycin A and semisynthetic tilmicosin) that possess the above mentioned identical catalytic scaffold, and the structure and antibiotic potential of two previously uncharacterized macrolides, 2,3-dihydro-oligomycin A (1) and 10,11-dihydro-tilmicosin (2), isolated from a S. venezuelae culture supplemented with oligomycin A and tilmicosin, respectively, were evaluated.A recombinant strain of S. venezuelae YJ028, 6 which had both the pikromycin polyketide synthase-encoding gene and desosamine biosynthetic genes deleted, was provided with oligomycin A or tilmicosin. The organism was cultivated in SCM media (1.5% soluble starch, 2.0% soytone, 0.01% CaCl 2 , 0.15% yeast extract, and 1.0% MOPS) in baffled Erlenmeyer flasks. After 2 days growth at 30 1C, the cultures were supplemented with either macrolides oligomycin A (Sigma, St Louis, MO, USA) and tilmicosin (Sigma) at a final concentration of 5 mg ml À1 , and then incubated for additional 3 days. The whole cultures were extracted and partitioned using EtOAc, and then the organic extracts were concentrated under vacuum. The dried residues were dissolved in 200 ml of MeOH and a portion of the solvent was subjected to HPL...

show abstract

Synthetic Biology of Antibiotic Production

Takano

Breitling

2014

Encyclopedia of Molecular Cell Biology and Molecular Medicine

View full text Add to dashboard Cite

ChemInform Abstract: Antimicrobial Natural Products: An Update on Future Antibiotic Drug Candidates

Cited by 118 publications

References 1 publication

Phytochemical screening and in vitro antimicrobial activity of Thymus lanceolatus Desf. from Algeria

Phytochemical screening and in vitro antimicrobial activity of Thymus lanceolatus Desf. from Algeria

Generation of reduced macrolide analogs by regio-specific biotransformation

Synthetic Biology of Antibiotic Production

Contact Info

Product

Resources

About