ChemInform Abstract: A Review on Biochemical and Therapeutic Aspects of Glutaminase

Unissa, Rahamat; Sudhakar, M.; Reddy, A. Sunil Kumar; Sravanthi, K

doi:10.1002/chin.201521304

Cited by 5 publications

(5 citation statements)

References 44 publications

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“…9 Many terrestrial fungi, including Penicillium, Phoma, Aspergillus, Phomopsis, Trichoderma, Cladosporium, Drechslera, Mucor, Chaetomium, and Emericella species, have been shown to produce L-glutaminase. [10][11][12][13][14][15][16] Apart from terrestrial fungus, marine fungus are also used to produce L-glutaminase. There are relatively few reports available on L-glutaminase production from marine fungi.…”

Section: Discussionmentioning

confidence: 99%

Production and Optimization of L-glutaminase from Halophilic Fusarium solani-melongenae Strain CRI 24 under Submerged and Solid State Fermentation

Vineetha,

Aldabaan,

et al. 2024

J. Pure Appl. Microbiol.

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L-glutaminase is a unique enzyme with catalytic activity and the ability to modulate glutamine levels, making it a valuable enzyme with numerous potential applications. L-glutaminase triggers a distinctive reaction by converting L-glutamine into glutamic acid while releasing ammonia concurrently. This enzymatic process holds potential applications across diverse industries, notably in food and pharmaceuticals. The primary objective of the present research was to identify and isolate a fungal strain proficient in L-glutaminase production from soil found in maritime environments. The physical and nutritional conditions were optimized for maximum synthesis of L-glutaminase under solid state fermentation (SSF) and submerged fermentation conditions (SmF). The isolated organism was identified as Fusarium solani-melongenae strain CRI 24 by morphological and 18S rRNA analysis. The optimum carbon source under SmF and SSF was found to be starch (0.2% w/v). Wheat bran as solid substrate among others showed optimum enzyme activity. On the seventh day of incubation, at pH 8.0 and 0.7% L-glutamine concentration under SSF and SmF, the highest enzyme activity was detected. The greatest enzyme activity in SSF was seen at a moisture content of 10%. Fusarium solani-melongenae species produced the enzyme under optimal conditions and 4.20 and 4.73-fold increase (from 0.8 U/mL to 3.61 U/mL and from 0.781 U/mL to 3.69 U/mL) was achieved after optimization in submerged and in solid state fermentation, respectively. The selective isolation and optimization processes described in this work are a promising technique for the industrial production of L-glutaminase and can be applied in the pharmaceutical and food industries.

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Section: Discussionmentioning

confidence: 99%

Production and Optimization of L-glutaminase from Halophilic Fusarium solani-melongenae Strain CRI 24 under Submerged and Solid State Fermentation

Vineetha,

Aldabaan,

et al. 2024

J. Pure Appl. Microbiol.

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“…Another advantage is that seawater and its salinity, which is closer to human blood plasma, can cause these fungi to produce biomolecules, especially enzymes that can cause lesser side effects during therapeutics 37 . Unissa et al 38 in their review listed out the various applications of L-glutaminase and the manufacture of thianine from L-glutaminase, which is an important amino acid infused in to tea to enhance its taste. They also observed that commercial production of L-glutaminase from Bacillus sp is commercialized in Japan and UK, though the production is done using yeasts and Aspergillus.…”

Section: Discussionmentioning

confidence: 99%

Bioprospecting of Marine Fungi from Coastal Karnataka Region as Potential Source of Economically Important Enzyme L-Glutaminase and their Comparative Genomic Study

Rao¹,

Sadananda²,

Pakkala³

et al. 2023

J. Pure Appl. Microbiol.

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Marine fungi are important sources of new metabolites including certain enzymes of medical interest due to their enormous capacity to adapt themselves to extreme environments. Living in a highly competitive ecological niche, they produce certain unusual chemical moieties. Marine biological resources are green, abundant, renewable and aid in economic development. The present study investigates the production of L-Glutaminase which is of therapeutic and industrial importance, from marine fungi of coastal Karnataka. Primary screening on agar plates and submerged fermentation in broth was employed for enzyme production. Both marine yeasts (Pichia sp) and filamentous fungal strains (Aspergillus, Penicillium) were found to be efficient producers of L-Glutaminase. Of the 42 isolates, five potential strains were selected through primary screening and Thin Layer Chromatography was performed to confirm the production. Filamentous fungi were identified through morphological and molecular methods as Penicillium and Aspergillus strains with 99-100% similarity. A. foveolatus (MT667385)and A. nidulans (MT667422) were potential producers (1.58U/ml and 1.41IU/ml). The yeast identified was Pichia kudriavzevii (MT667428), which was a moderate producer of Glutaminase and first marine yeast reported for this enzyme production. Neosartorya quadricincta (MT667427) and P. citrinum (MT667426) are also moderate producers. After screening the marine fungi, the isolated strains’ potential to produce L-Glutaminase was confirmed using SDS PAGE, FTIR and Mass analysis. This study emphasizes the necessity of marine fungal culturing and the scope of use of these fungi for further commercial production of L-Glutaminase which would uplift marine economy.

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“…It involves in nutritional starvation therapy for treatment of human hepatocellular carcinoma, prostate cancer, and melanoma. In addition to anticancer activity, it was proved to be effective in the treatment of acute neurological disorders, rheumatoid arthritis and allergic asthma [36].…”

Section: Production Of Enzymes With Biomedical Applications By Arthromentioning

confidence: 99%

Arthrobacter as Biofactory of Therapeutic Enzymes

Azmi¹,

Chaudhary²

2018

Int J Pharm Pharm Sci

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Therapeutic enzymes are proteins which can be used to treat rare and deadly diseases. They represent a small but profitable market. Therapeutic enzymes are superior to non-enzymatic drugs owing to their high specificity toward the target and also their ability to multiple substrate conversion. They are essential for speeding up all the metabolic processes and many a life-supporting chemical inter-conversions. Actinomycetes including Arthrobacter form an enormous reservoir of secondary metabolites and enzymes. The characterization of L-asparaginase, β-glucosidase, urate oxidase, methionine γ-lyase, acetyl cholinesterase, and arginase activities from actinomycetes Arthrobacter clearly demonstrate the potential of Arthrobacter as potent producer of therapeutic enzymes. These metabolic enzymes can be used either separately or in combination with other therapies for the treatment of several diseases such as leukemia, gout, asthma, and neurological disorders. The objective of this review is to compile the information on the application of therapeutic enzymes produced by Arthrobacter and their future prospects as drugs.

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ChemInform Abstract: A Review on Biochemical and Therapeutic Aspects of Glutaminase

Abstract: Review: 128 refs.

Cited by 5 publications

References 44 publications

Production and Optimization of L-glutaminase from Halophilic Fusarium solani-melongenae Strain CRI 24 under Submerged and Solid State Fermentation

Production and Optimization of L-glutaminase from Halophilic Fusarium solani-melongenae Strain CRI 24 under Submerged and Solid State Fermentation

Bioprospecting of Marine Fungi from Coastal Karnataka Region as Potential Source of Economically Important Enzyme L-Glutaminase and their Comparative Genomic Study

Arthrobacter as Biofactory of Therapeutic Enzymes

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