Chemiluminometric Sensor for Simultaneous Determination of <scp>l</scp>-Glutamate and <scp>l</scp>-Lysine with Immobilized Oxidases in a Flow Injection System

Kiba, Nobutoshi; Miwa, Takao; Terabe, Masaki; Tani, Kazue; Koizumi, Hideaki

doi:10.1021/ac011013d

Cited by 40 publications

(24 citation statements)

References 19 publications

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“…Recall that the dielectric constant (ε) of a metal (m) is a complex quantity given by (3) where and the subscripts indicate the real (r) and imaginary (im) components. As an example, the dielectric constant of gold at 600 nm is near ε m = −10.5 + 1.49i.…”

Section: Theorymentioning

confidence: 99%

“…For example, chemiluminescence (CL) and electrochemiluminescence (ECL) can provide a 5-100-fold greater sensitivity than fluorescence [1]. As a result, CL and ECL are widely used in medical testing [2][3][4][5]. ECL methods have been developed for a variety of applications including capillary electrophoresis [6,7], detection of ions [8], organic acids [9] and explosives [10].…”

Section: Introductionmentioning

confidence: 99%

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First observation of surface plasmon-coupled electrochemiluminescence

Zhang

Gryczyński

Lakowicz

2004

Chemical Physics Letters

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Section: Theorymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

First observation of surface plasmon-coupled electrochemiluminescence

Zhang

Gryczyński

Lakowicz

2004

Chemical Physics Letters

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“…The performances of the present analytical systems are competitive with previously described method [6,8,9,12,13,14,15,16,17,18] and their extension to complex systems composed of nucleic acid or peptide library is currently under development. A number of parameters have to be optimised in order to develop such multiplexed systems, in particular the hybridisation conditions of multiple oligonucleotide sequences and the cross-reactivity and the non-specific binding of arrayed immunotests.…”

Section: Resultsmentioning

confidence: 81%

“…Glucose detection could be performed in the range 20 µmol L -1 to 2 mmol L -1 with a detection limit of 20 µmol L -1 , and lactate and choline in the range 2 µmol L -1 to 0.2 mmol L -1 with detection limits of 2 µmol L -1 . When compared to the performances of the previously described mono-parameter biosensors for the detection of those six compounds [6,8,9,12,13,14], the present biochip exhibits equivalent detection ranges and sensitivities, with an acceptable total measurement time of 3 min.…”

Section: Resultsmentioning

confidence: 95%

Design of luminescent biochips based on enzyme, antibody, or DNA composite layers

Marquette

Thomas

Degiuli

et al. 2003

Analytical and Bioanalytical Chemistry

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The use of beads bearing bioactive molecules to develop generic biochips based on chemi- and electro-chemiluminescent detection was evaluated. The biochips were composed of arrayed biosensors, including enzyme-charged beads, antigen-charged beads, or oligonucleotide-charged beads, entrapped in poly(vinyl alcohol) (PVA-SbQ) photopolymer. In each case the sensing layers were spotted at the surface of a glassy carbon electrode as 0.3 microL drops, generating 500-800 microm spots. The luminescent reactions were either catalysed by horseradish peroxidase or triggered by application of a +850 mV potential between the glassy carbon electrode and a platinum pseudo-reference. Enzyme biochips were designed for the concomitant detection of choline, glucose, glutamate, lactate, lysine, and urate, based on the corresponding oxidase-charged beads and the electro-chemiluminescent (ECL) reaction with luminol-immobilised beads of the hydrogen peroxide produced. Limits of detection of 1 micromol L(-1) for glutamate, lysine and uric acid, 20 micromol L(-1) for glucose, and 2 micromol L(-1) for choline and lactate were found with detection ranging over three decades at least. Use of the electro-chemiluminescent biochip was extended to a tri-enzymatic sensing layer based on kinase-oxidase activity for detection of acetate. A reaction sequence using acetate kinase, pyruvate kinase, and pyruvate oxidase enabled the production of H2O2 in response to acetate injection in the range 10 micromol L(-1) to 100 mmol L(-1). Based on IgG-bearing beads, a chemiluminescent immuno-biochip has been also realised for the model detection of human IgG. Biotin-labelled anti-human IgG were used in a competitive assay, in conjunction with peroxidase-labelled streptavidin. Free antigen could then be detected with a detection limit of 25 pg (10(8) molecules) and up to 15 ng. In a similar way, the use of oligonucleotide-immobilised beads enabled the realisation of DNA-sensitive biochips which could be used to detect a biotin-labelled sequence al a level of 5x10(8) molecules.

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“…[22][23][24] We have recently proposed chemiluminometric biosensors for simultaneous determination of oxidase substrates on the basis of integration of oxidase reactions and peroxidase (POx)-catalyzed H2O2/luminol detection reactions, which involved no separation process. 25,26 This paper describes the performance of a rapid and sensitive flow-through sensor for the simultaneous determination of ACh and Ch without separation of the analytes. The sensor was based on the integration of ChOx and AChE reactions, and the POx catalyzed luminol chemiluminescence (CL) detection reactions.…”

mentioning

confidence: 99%

Simultaneous Determination of Choline and Acetylcholine Based on a Trienzyme Chemiluminometric Biosensor in a Single Line Flow Injection System

et al. 2003

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The development of methods for rapid and sensitive determination of neurotransmitters and their precursors and/or metabolites has been a challenging goal for many years since they provide valuable information for disposition dynamics rather than the static levels of neurotransmitters. Acetylcholine (ACh), a main neurotransmitter in both the peripheral and central nervous systems, is found in mammalian brain tissues. Despite intensive research, the roles of neurotransmitters in the central nervous system are much less delineated, although it is generally considered that ACh is involved in temperature and blood pressure regulation, motor coordination, learning, memory, etc.1 Furthermore, ACh and choline (Ch) levels in the brain are very important due to the significant role in the neuropsychiatric diseases; 2-6 Ch serves both as the precursor and metabolite of ACh.Numerous chromatographic methods have been described for the simultaneous measurement of Ch and ACh. Though high performance liquid chromatography (HPLC) coupled with MS or with tandem MS required minimal sample preparation and no derivatization, it had relatively inadequate quantitation limits 9-12 HPLC-electrochemical detection (ECD) with immobilized acetylcholinesterase (EC 3.1.1.7, AChE) and Ch oxidase (EC 1.1.3.7, ChOx) reactors as column reactor 13,14 or with coimmobilized AChE/ChOx bienzyme electrode 15 were suited for the sensitive determinations of Ch and ACh; the detection limits and sample throughput have been reported to be 1 and 3 nM for Ch and ACh, respectively, and 3/h. 15 The slower flow rate is preferable to the conversion efficencies for the analytes to H2O2 and to the sensitive detection of the H2O2, but the decreased sample throughput is undesirable to follow minutely the dynamics of ACh and Ch.For the rapid assays, though there have been many flow injection (FI) methods with immobilized ChOx or coimmobilized AChE/ChOx for the determination of Ch or ACh, 16-20 a split-steam FI system with chemiluminometric detection using three immobilized enzyme column reactors such as an immobilized ChOx, an immobilized catalase and a coimmobilized AChE/ChOx, and using ferricyanide-catalyzed luminol reaction have been developed for simultaneous determination of Ch and ACh. 21In a split-stream FI system with column reactors, generally, sample plugs from the reactors disperse into the carrier stream and are, moreover, diluted with the carrier solution from other streams; the resulting peak broadening causes lowering of sensitivity and sample throughput. Flow-through sensors based on the integration of conversion reaction and detection reaction in a flow cell have been designed to solve the problems. [22][23][24] We have recently proposed chemiluminometric biosensors for simultaneous determination of oxidase substrates on the basis of integration of oxidase reactions and peroxidase (POx)-catalyzed H2O2/luminol detection reactions, which involved no separation process. 25,26 This paper describes the performance of a rapid and sensitive flow-through sen...

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Chemiluminometric Sensor for Simultaneous Determination of l-Glutamate and l-Lysine with Immobilized Oxidases in a Flow Injection System

Cited by 40 publications

References 19 publications

First observation of surface plasmon-coupled electrochemiluminescence

First observation of surface plasmon-coupled electrochemiluminescence

Design of luminescent biochips based on enzyme, antibody, or DNA composite layers

Simultaneous Determination of Choline and Acetylcholine Based on a Trienzyme Chemiluminometric Biosensor in a Single Line Flow Injection System

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