Chemiluminescent microplate-based assay of DNA based on isothermal circular strand-displacement polymerization reaction (ICSDPR)

Solovjev, Anton M.; Kurzeev, S. A.; Sakharov, I Iu

doi:10.1016/j.talanta.2020.120895

Cited by 12 publications

(7 citation statements)

References 33 publications

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“…First, 96-well black microplates with adsorbed anti-FluAb were prepared as described in [ 20 ]. For this, 50 μL of 6 µg/mL monoclonal anti-FluAb in 50 mM carbonate buffer, pH 9.5 was added to the plate wells and incubated for 20 h at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

Quantitation of MicroRNA-155 in Human Cells by Heterogeneous Enzyme-Linked Oligonucleotide Assay Coupled with Mismatched Catalytic Hairpin Assembly Reaction

et al. 2022

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In the present work, we describe the development of a chemiluminescent enzyme-linked oligonucleotide assay coupled with mismatched catalytic hairpin assembly (mCHA) amplification for the quantitative determination of microRNA-155. To improve its sensitivity, a polymerase-free mCHA reaction was applied as an isothermal amplification method. The detection limit of the proposed assay was 400 fM. In addition, the high specificity of the assay was demonstrated. The proposed assay allowed assessment of the content of microRNA-155 in human cancer lines such as HepG2, Caco2, MCF7, and HeLa. The quantitation of microRNA-155 was performed after purification of short RNAs (less than 200 nt) from cell lysates since a high matrix effect was observed without this pre-treatment. The results of the quantitative determination of the microRNA content in cells were normalized using nematode microRNA-39, the concentration of which was determined using a heterogeneous assay developed by us using a strategy identical to that of the microRNA-155 assay.

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Section: Methodsmentioning

confidence: 99%

Quantitation of MicroRNA-155 in Human Cells by Heterogeneous Enzyme-Linked Oligonucleotide Assay Coupled with Mismatched Catalytic Hairpin Assembly Reaction

et al. 2022

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“…The FASP method utilizes a molecular weight cutoff (MWCO) membrane to filter out digested peptides above a certain molecular weight for downstream analysis and allows for all sample preparation steps in a proteomic analysis to be carried out in a single 96-well microplate [ 153 , 154 , 155 ]. Building off of the microplate-based diagnostic assay and integrated sample preparation concepts, Solovjev et al have developed a microplate-based, chemiluminescent NAAT assay [ 156 ], and Nichols et al have developed a microplate designed to prepare clinical samples for detection via lysis by microwave heating [ 157 ]. As observed with POC-focused methods, smart devices have also demonstrated clinical utility as mobile microplate readers for diagnostic assays, as reported by Wang et al [ 158 ].…”

Section: High-throughput Diagnostic Methods In Laboratory Settingsmentioning

confidence: 99%

“… Microplate-based methods for high-throughput and automated sample preparation: ( a ) magnetic beads used for high-throughput separations or magnetic pelleting; ( b ) MStern blot adsorption of proteins to the well for separation; ( c ) filter-aided sample processing (FASP) for separating proteins using molecular weight cutoff (MWCO); ( d ) in-tip digestion of protein samples using an automated, modified pipette and a 96-well plate robot. From Mafra et al [ 147 ], Berger et al [ 152 ], Switzar et al, Yu et al, Wisniewski et al [ 153 , 154 , 155 ], and Yang et al [ 41 , 156 ]. …”

Section: Figurementioning

confidence: 99%

Sample Preparation and Diagnostic Methods for a Variety of Settings: A Comprehensive Review

Nichols

Geddes

2021

Molecules

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Sample preparation is an essential step for nearly every type of biochemical analysis in use today. Among the most important of these analyses is the diagnosis of diseases, since their treatment may rely greatly on time and, in the case of infectious diseases, containing their spread within a population to prevent outbreaks. To address this, many different methods have been developed for use in the wide variety of settings for which they are needed. In this work, we have reviewed the literature and report on a broad range of methods that have been developed in recent years and their applications to point-of-care (POC), high-throughput screening, and low-resource and traditional clinical settings for diagnosis, including some of those that were developed in response to the coronavirus disease 2019 (COVID-19) pandemic. In addition to covering alternative approaches and improvements to traditional sample preparation techniques such as extractions and separations, techniques that have been developed with focuses on integration with smart devices, laboratory automation, and biosensors are also discussed.

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“…At the same time, amplification is carried out under both homogeneous and heterogeneous conditions. [20][21][22][23] The results reported previously have indicated that both approaches permit the development of highly sensitive assays of miRNAs. However, no direct comparative study of these two formats has been carried out, which leaves unanswered the question of which of these formats of amplification, homogeneous or heterogeneous, is preferable to use.…”

Section: Introductionmentioning

confidence: 99%

Comparison of chemiluminescent heterogeneous and homogeneous–heterogeneous assays coupled with isothermal circular strand-displacement polymerization reaction amplification for the quantification of miRNA-141

Solovjev

Galkin

Медведько

et al. 2022

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Chemiluminescent microplate-based assay of DNA based on isothermal circular strand-displacement polymerization reaction (ICSDPR)

Cited by 12 publications

References 33 publications

Quantitation of MicroRNA-155 in Human Cells by Heterogeneous Enzyme-Linked Oligonucleotide Assay Coupled with Mismatched Catalytic Hairpin Assembly Reaction

Quantitation of MicroRNA-155 in Human Cells by Heterogeneous Enzyme-Linked Oligonucleotide Assay Coupled with Mismatched Catalytic Hairpin Assembly Reaction

Sample Preparation and Diagnostic Methods for a Variety of Settings: A Comprehensive Review

Comparison of chemiluminescent heterogeneous and homogeneous–heterogeneous assays coupled with isothermal circular strand-displacement polymerization reaction amplification for the quantification of miRNA-141

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