Chemiluminescent method for detecting microorganisms in water

Oleniacz, W; Pisano, Michael A.; Rosenfeld, Martin H.; Elgart, Robert L.

doi:10.1021/es60022a006

Cited by 25 publications

(11 citation statements)

References 6 publications

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“…Sediment samples were diluted with membrane-filtered water before staining. Bacteria in water were also detected by the chemiluminescent method of Oleniacz et al (1968) using membrane filtered samples as controls.…”

Section: Direct Counts and Chemiluminescence Determinations Of Bacteriamentioning

confidence: 99%

The Measurement of Electron Transport System Activity in Freshwater Benthic and Planktonic Samples

Jones

Simon

1979

Journal of Applied Bacteriology

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The measurement of electron transport system (ETS) activity in freshwater benthic and planktonic samples was examined. Significant improvements in sensitivity were obtained by increasing the concentration of substrates and of Triton X-100 in the extraction buffer and by changing the buffer system. The mechanical homogenization procedures which have been used previously to extract the enzymes were shown to be inadequate yielding, at best, 25% of the activity obtained by ultrasonication. The yield of enzymes from sediments was greatly affected by the degree of dilution used and previously published methods would appear to be inadequate.ETS activity correlated well with biological oxygen uptake and, in individual water and sediment profiles, with microbial numbers and biomass. At a more general level, however, the ETS :biomass ratio showed considerable variation.

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Section: Direct Counts and Chemiluminescence Determinations Of Bacteriamentioning

confidence: 99%

The Measurement of Electron Transport System Activity in Freshwater Benthic and Planktonic Samples

Jones

Simon

1979

Journal of Applied Bacteriology

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“…The application of luminol chemiluminescence in bacterial enumeration has been reported before in environmental (Oleniacz et al 1968) and clinical (Ewetz and Strangert 1974) settings. In these studies, 0.2 N NaOH was used as a medium for maintaining the required basic condition and at the same time lyse the bacteria and release the analyte of interest.…”

Section: Resultsmentioning

confidence: 96%

Comparison of Luminol Chemiluminescence With Atp Bioluminescence for the Estimation of Total Bacterial Load in Pure Cultures

Pietrzak¹,

Denes²

1996

Rapid Methods Automation Mic

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A rapid chemiluminescent assay of total bacterial load that is based on the oxidation of luminol (5‐amino‐2,3‐dihydro‐1,4‐phthalazinedione) as catalyzed by bacterial iron protoporphyrins is described and compared to the ATP bioluminescent assay of microbial biomass. An assay format that elicits linear light output response to a range of analyte concentrations of model compounds such as hematin and various heme‐containing enzymes within the dynamic range of a BioOrbit 1251 luminometer is presented. When the assay was applied to eight pure bacterial cultures, the sensitivity was typically in the range of 104‐105 cfu/ml, and was comparable to that obtained by the ATP assay. Similar levels of sensitivity can be derived from estimates of average values of 2.8 × 10‐18 mole of heme/cfu and 1 × 10‐19 mole of ATP/cfu. The potential of the luminal assay as an alternative rapid test for the estimation of total bacterial count in food and environmental samples is discussed.

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“…The luminol‐enhanced chemiluminescence method described has been used for sensitive detection of bacteria in various media (Oleniacz et al . 1968; Ewetz and Strangert 1974).…”

Section: Introductionmentioning

confidence: 99%

“…The luminol-enhanced chemiluminescence method described has been used for sensitive detection of bacteria in various media (Oleniacz et al 1968;Ewetz and Strangert 1974). It was ®rst proposed that the chemiluminescence reaction is dependant on the protohemin content of the micro-organisms (Sotnikov 1970).…”

Section: Introductionmentioning

confidence: 99%

A simple chemiluminescence-based method for rapid enumeration ofListeriaspp. microcolonies

Vidon¹,

Donze²,

Muller³

et al. 2001

Journal of Applied Microbiology

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Aims: Listeria monocytogenes is capable, under certain conditions, of producing chemiluminescence which is ampli®ed by luminol. This property was used to detect and count microcolonies of Listeria spp. in a few hours, without the use of a microscope. Methods and Results: After trapping Listeria cells on polyvinylidene¯uoride membranes, a chemiluminescence mixture was sprayed onto the membrane. The chemiluminescent spots emitted were analysed by a charge-coupled device camera connected to a data-processing system, which restored the intensity of the signals into three dimensional images. The intensity of the luminescence of microcolonies was improved by addition of cellobiose, and by brief exposure to u.v. light. Conclusions: Microcolonies of Listeria spp. can be imaged and counted by luminol-enhanced chemiluminescence with a photon-counting system. Signi®cance and Impact of the Study: This method can be applied to the rapid detection and counting of Listeria spp. in raw milk.

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Chemiluminescent method for detecting microorganisms in water

Cited by 25 publications

References 6 publications

The Measurement of Electron Transport System Activity in Freshwater Benthic and Planktonic Samples

The Measurement of Electron Transport System Activity in Freshwater Benthic and Planktonic Samples

Comparison of Luminol Chemiluminescence With Atp Bioluminescence for the Estimation of Total Bacterial Load in Pure Cultures

A simple chemiluminescence-based method for rapid enumeration ofListeriaspp. microcolonies

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