Chemiluminescent labels for steroid immunoassays

Klingler, W.; Wiegand, G.; Knüppen, R.

doi:10.1016/0022-4731(87)90292-5

Cited by 11 publications

(1 citation statement)

References 28 publications

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“…A necessidade de ensaios simples e reprodutíveis foi sempre um fator a estimular desenvolvimentos tecnológicos em todas as áreas diagnósticas, inclusive no caso dos imunoensaios para esteróides hormonais. Processos preparativos cada vez menos elaborados, emprego de traçadores não triciados (radioativos ou não), emprego de métodos de separação mais reprodutíveis e simples (como fase sólida), foram sendo introduzidos ao longo dos anos (5,6). A limitação para tal desenvolvimento foi a disponibilidade de anticorpos mais específicos e o desenvolvimento de processos preparativos.…”

Section: Introductionunclassified

Aplicação da Cromatografia Líquida de Alta Performance Como Método Preparativo Para a Dosagem de Esteróides Hormonais Por RIE: Dosagens de 17OH-Progesterona e Diidrotestosterona

Vieira

Nakamura

Noguti

2002

Arq Bras Endocrinol Metab

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Preparative HPLC for Steroid Measurements: Determinations of 170H-Progesterone and Dihydrotestosterone. Measurement of steroid hormones has undergone a significant technical evolution so that steroids with greater clinical interest are, nowadays, measured with methods that are simple, fast and prone to automation. Nevertheless, a compromise is made in detriment of accuracy, which is more evident with steroids with lower concentration and more potential interferents, as 17-hydroxyprogesterone (17OHP), dihydrotestosterone (DHT), and others. In such cases, a robust and versatile preparative process is of utmost importance to warrant results with the highest degree of accuracy. In this paper we present the results obtained with the use of a preparative process based on high-pressure chromatography (HPLC) for the measurement of 17OHP and DHT, as compared with a process based on celite column chromatography. The antibodies used in the radioimmunoassays have similar specificity to those previously described for these kinds of assays. Samples were initially extracted in ethyl ether and then submitted to HPLC. Routine samples (57 for 17OHP and 84 for DHT) were measured in parallel with both methods. The differences between results were not statistically significant, and the correlation indexes were high (R= 0.95 and 0.97). Our results confirm that systems

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Section: Introductionunclassified

Aplicação da Cromatografia Líquida de Alta Performance Como Método Preparativo Para a Dosagem de Esteróides Hormonais Por RIE: Dosagens de 17OH-Progesterona e Diidrotestosterona

Vieira

Nakamura

Noguti

2002

Arq Bras Endocrinol Metab

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Lumineszenzmarker – mehr als nur eine Alternative zu Radioisotopen?

Mayer¹,

Neuenhofer²

1994

Angewandte Chemie

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Luminescent Labels—More than Just an Alternative to Radioisotopes?

Mayer

Neuenhofer²

1994

Angew. Chem. Int. Ed. Engl.

212

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Chemical, chromatographic, or spectrometric methods are generally unsuitable for the detection of molecules in the nano and subnanogram region because of their low sensitivity. The radioimmunoassay (RIA) developed by Yalow and Berson in 1959 combined the high sensitivity of radioactively labeled substances with the high specificity of immunological reactions for the first time. In this way it was possible to detect quantitatively the tiniest traces of substances in the presence of an excess of other, in some cases, similar foreign substances without prior enrichment. Immunoassays have certainly developed to become the most valuable analytical tool of in vitro diagnostics and are today routinely employed for the detection of endogenous and exogenous substances (e.g. hormones, tumor‐associated proteins, bacteria, viruses, toxins, drugs, etc). The many disadvantages of radioactivity such as the required handling licenses, disposal costs, precautions necessary to prevent risks to health, short shelf‐life, and limited sensitivity soon led to the search for other nonradioactive labeling methods. Encouraged by the development of light measuring techniques and the commercial availability of highly sensitive apparatus, radioactive isotopes as labels are today being replaced increasingly by enzymes, fluorophores, or luminophores. Some of the new luminescent labels have, however, not only facilitated replacement of radioisotopes, but also a breakthrough into what has until now been unattainable levels of sensitivity. The following article reviews the methods of luminescent labeling and their applications mainly in the area of immunoassays.

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Chemiluminescent labels for steroid immunoassays

Cited by 11 publications

References 28 publications

Aplicação da Cromatografia Líquida de Alta Performance Como Método Preparativo Para a Dosagem de Esteróides Hormonais Por RIE: Dosagens de 17OH-Progesterona e Diidrotestosterona

Aplicação da Cromatografia Líquida de Alta Performance Como Método Preparativo Para a Dosagem de Esteróides Hormonais Por RIE: Dosagens de 17OH-Progesterona e Diidrotestosterona

Lumineszenzmarker – mehr als nur eine Alternative zu Radioisotopen?

Luminescent Labels—More than Just an Alternative to Radioisotopes?

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