Chemiluminescent Identification and Quantification of Artemisinin and Relevant Sesquiterpene Lactone Derivatives

Bowles, David J; Olson, Andrew S.; Hodges, Neal H.; Heglund, Daniel L; Vargas, Roberto L.; Williams, Scott

doi:10.1366/11-06462

Cited by 9 publications

(8 citation statements)

References 19 publications

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“…It can be quantified using luminol and hematin in the sodium hydroxide solution. 26 Before the probe can be used to test drug samples, a series of optimization and validation tests (the limit of detection/quantification (LOD/LOQ), specificity, repeatability, and robustness) was performed using the spectrophotometer. To make the platform field deployable, a microfluidic chip was designed to carry the mixing of the probe and API and their luminescent reaction.…”

Section: Methodsmentioning

confidence: 99%

Rapid and Specific Drug Quality Testing Assay for Artemisinin and Its Derivatives Using a Luminescent Reaction and Novel Microfluidic Technology

Ho¹,

Desai²,

Zaman³

2015

The American Journal of Tropical Medicine and Hygiene

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Globally, it is estimated that about 10–30% of pharmaceuticals are of poor quality. Poor-quality drugs lead to long-term drug resistance, create morbidity, and strain the financial structure of the health system. The current technologies for substandard drug detection either are too expensive for low-resource regions or only provide qualitative results. To address the current limitations with point-of-care technologies, we have developed an affordable and robust assay to quantify the amount of active pharmaceutical ingredients (APIs) to test product quality. Our novel assay consists of two parts: detection reagent (probe) and a microfluidic testing platform. As antimalarials are of high importance in the global fight against malaria and are often substandard, they are chosen as the model to validate our assay. As a proof-of-concept, we have tested the assay with artesunate pure and substandard samples (Arsuamoon tablets) from Africa and compared with the conventional 96-well plate with spectrophotometer to demonstrate the quantitative efficacy and performance of our system.

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Section: Methodsmentioning

confidence: 99%

Rapid and Specific Drug Quality Testing Assay for Artemisinin and Its Derivatives Using a Luminescent Reaction and Novel Microfluidic Technology

Ho¹,

Desai²,

Zaman³

2015

The American Journal of Tropical Medicine and Hygiene

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“…On the other hand, as an advanced technique of analysis, chemiluminescence (CL) has received remarkable attention. [18][19][20][21][22][23][24][25] It is characterized by high sensitivity and simple instrumentation compared with other spectrophotometric techniques. In comparison with other optical detection methods such as fluorescence, Raman spectroscopy and absorption, the process of CL has a wide linear range, uses an inexpensive apparatus, has a fast response time, high sensitivity, and no interference from background scattering light.…”

Section: Introductionmentioning

confidence: 99%

An in-fiber integrated optofluidic device based on an optical fiber with an inner core

Yang

Yuan

Teng³

et al. 2014

Lab Chip

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A new kind of optofluidic in-fiber integrated device based on a specially designed hollow optical fiber with an inner core is designed. The inlets and outlets are built by etching the surface of the optical fiber without damaging the inner core. A reaction region between the end of the fiber and a solid point obtained after melting is constructed. By injecting samples into the fiber, the liquids can form steady microflows and react in the region. Simultaneously, the emission from the chemiluminescence reaction can be detected from the remote end of the optical fiber through evanescent field coupling. The concentration of ascorbic acid (AA or vitamin C, Vc) is determined by the emission intensity of the reaction of Vc, H2O2, luminol, and K3Fe(CN)6 in the optical fiber. A linear sensing range of 0.1-3.0 mmol L(-1) for Vc is obtained. The emission intensity can be determined within 2 s at a total flow rate of 150 μL min(-1). Significantly, this work presents information for the in-fiber integrated optofluidic devices without spatial optical coupling.

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“…On the other hand, as an advanced technique of analysis, chemiluminescence (CL) is greatly attractive in miniaturized analytical systems [8][9] . It is characterized by high sensitivity and simple instrumentation compared with other spectrophotometric techniques.…”

Section: Introductionmentioning

confidence: 99%

In-fiber integrated microfluidic sensor based on optical fiber with suspended core

et al. 2014

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We report an in-fiber integrated chemiluminiscence sensor based on a kind of hollow optical fiber with a suspended inner core. The path of mircofluid is realized by etching microholes for inlets and outlets on the surface of the optical fiber without damaging the inner core and then constructing a melted point beside the microhole of the outlet. By injecting samples into the fiber, the liquids can be fully mixed and form steady microflows. Simultaneously, the photon emitted from the chemiluminiscence reaction is efficiently coupled into the core and can be detected at the end of the optical fiber.

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Chemiluminescent Identification and Quantification of Artemisinin and Relevant Sesquiterpene Lactone Derivatives

Cited by 9 publications

References 19 publications

Rapid and Specific Drug Quality Testing Assay for Artemisinin and Its Derivatives Using a Luminescent Reaction and Novel Microfluidic Technology

Rapid and Specific Drug Quality Testing Assay for Artemisinin and Its Derivatives Using a Luminescent Reaction and Novel Microfluidic Technology

An in-fiber integrated optofluidic device based on an optical fiber with an inner core

In-fiber integrated microfluidic sensor based on optical fiber with suspended core

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