Chemiluminescent determination of leukocyte alkaline phosphatase: an advantageous alternative to the cytochemical assay

Kanegae, Marilia P. P.; Ximenes, Valdecir Farias; Falcão, Roberto Passetto; Colturato, Virgílio Antônio Rensi; Mattos, E.R.; Brunetti, Iguatemy Lourenço; Fonseca, Luiz Marcos da

doi:10.1002/jcla.20140

Cited by 5 publications

(2 citation statements)

References 21 publications

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“…3 mL of venous blood was used to obtain leukocytes: blood was added to 1 mL of 10% EDTA and 6% Dextran (T500) [20]. The supernatant was removed after 1 h, divided into two tubes, and centrifuged at 200 g for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Detection of DNA from Leishmania (Viannia): Accuracy of Polymerase Chain Reaction for the Diagnosis of Cutaneous Leishmaniasis

et al. 2013

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Cutaneous leishmaniasis (CL) can occur in skin and mucosa, causing disfiguring lesions. The laboratory diagnosis of CL involves immunological methods and optical detection of the parasite, al of which have limitations. There is a need for more effective diagnostic methods for CL which wil allow treatment to be initiated more promptly in order to help prevent the development of severe forms of mucosal disease, and to estimate the prognosis of the infection. The polymerase chain reaction (PCR) has been widely used to diagnose CL, because of its higher sensitivity. This study estimated the accuracy and compared PCRs of samples from lesion scarification (PCR-L) and blood sample-enriched leukocytes (PCR-B) with three conventional diagnostic techniques: parasite direct search (DS), Montenegro skin test (MST), and indirect immunofluorescence reaction (IIF). The study included 276 patients under suspicion of CL. We conducted a cross-sectional study, in which patients were selected by convenience sampling. We used MP3H/MP1L primers to generate a Leishmania (Viannia) (minicircle kDNA) fragment of 70-bp. Of 106 patients with CL, 83.87%, 51.67%, 64.52%, 85.71%, or 96.10% tested positive by PCR-L, PCR-B, DS, IIF, or MST, respectively. Five patients tested positive only by PCR-L, and two other patients only by PCR-B. PCR-L is indicated for use in patients with chronic lesions or Leishmania reinfection, which may progress to mucosal lesion. PCR-B is indicated for use in patients with negative results in conventional tests or for patients with no apparent lesion. PCR is not only useful in diagnosing CL but also helps to identify the infecting species.

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Section: Methodsmentioning

confidence: 99%

Detection of DNA from Leishmania (Viannia): Accuracy of Polymerase Chain Reaction for the Diagnosis of Cutaneous Leishmaniasis

et al. 2013

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“…Human chronic myelogenous leukemia (CML), a malignancy of pluripotent hematopoietic cells, seriously affects patients’ bone marrow, peripheral blood and lymphatic system . Traditional detection methods for CML include bone marrow examination , cytochemistry test , immunophenotyping or bone marrow minimal residual disease examination . However, these methods have a low specificity and a bone marrow aspiration must be required, which is a huge pain for the patients .…”

Section: Introductionmentioning

confidence: 99%

A Simple Electrochemical Aptamer Cytosensor for Direct Detection of Chronic Myelogenous Leukemia K562 Cells

Zhang

Huang

et al. 2016

Electroanalysis

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A simple and rapid electrochemical aptamer cytosensor has been developed for direct detection of chronic myelogenous leukemia (CML) K562 cells based on a specific aptamer and a biotin conjugated concanavalin A (bio‐ConA) detection probe. The K562 cell could be specifically recognized by T2‐KK1B10 capture aptamer pre‐immobilized on gold modified electrode surface. Then, bio‐ConA was added in the reaction to identify K562 cell surface mannose, resulting in an aptamer‐K562 cell‐bio‐ConA sandwich complex. Finally, streptavidin conjugated alkaline phosphatase (ST‐ALP) combined with the bio‐ConA to catalyze α‐naphthyl (α‐NP) phosphate to form α‐naphthol which is highly electroactive at an operating voltage of 180 mV (vs. Ag/AgCl). Under optimum conditions, the DPV signals were proportional to the logarithm of K562 cell from 1×102 to 1×107 cells mL−1 with a detection limit of 79 cells mL−1. The cytosensor also exhibited high selectivity, stability and reproducibility. When applied to detect K562 cells in human blood samples, recoveries between 79.6 %–93.3 % were obtained, indicating the developed biosensor would be a potential alternative tool for CML K562 cell detection in real biological samples.

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Four-Membered Rings With Two Oxygen Atoms

Pradal

2022

Comprehensive Heterocyclic Chemistry IV

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Chemiluminescent determination of leukocyte alkaline phosphatase: an advantageous alternative to the cytochemical assay

Cited by 5 publications

References 21 publications

Detection of DNA from Leishmania (Viannia): Accuracy of Polymerase Chain Reaction for the Diagnosis of Cutaneous Leishmaniasis

Detection of DNA from Leishmania (Viannia): Accuracy of Polymerase Chain Reaction for the Diagnosis of Cutaneous Leishmaniasis

A Simple Electrochemical Aptamer Cytosensor for Direct Detection of Chronic Myelogenous Leukemia K562 Cells

Four-Membered Rings With Two Oxygen Atoms

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