Chemiluminescence “turn-on” detection of tyrosinase activity <i>via in situ</i> generation of dopamine based on a lucigenin and riboflavin system

Wang, Yi; Du, Chengpei; Liu, Ze; Pei, Kanglin; Zhang, Yan; Qi, Wenjing

doi:10.1039/d1nj05628j

Cited by 6 publications

(2 citation statements)

References 37 publications

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“…Lucigenin was reported by Decker and Dunant in 1906 [22] and was first investigated for its chemiluminescence. [23][24][25] The chemiluminescent properties of lucigenin are currently used for the detection of various biologically relevant substances [26][27][28][29] and the detection of superoxide. [30][31][32] 10.1002/anse.202300044…”

Section: Optical Properties Of Lucigeninmentioning

confidence: 99%

The Lucigenin Assay: Measuring Anion Transport in Lipid Vesicles

Chvojka,

Singh,

Cataldo

et al. 2024

Analysis & Sensing

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Invited for this month′s cover are the collaborating groups of the Université libre de Bruxelles and Masaryk University. The cover picture shows liposomes with a fluorescent probe encapsulated to study the transmembrane transport of chloride by synthetic anion transporters via quenching of the fluorescence. More information can be found in the Concept article by Hennie Valkenier and co‐workers.

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Section: Optical Properties Of Lucigeninmentioning

confidence: 99%

The Lucigenin Assay: Measuring Anion Transport in Lipid Vesicles

Chvojka,

Singh,

Cataldo

et al. 2024

Analysis & Sensing

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Section: Optical Properties Of Lucigeninmentioning

confidence: 99%

The Lucigenin Assay: Measuring Anion Transport in Lipid Vesicles**

Chvojka,

Singh,

Cataldo

et al. 2023

Analysis & Sensing

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Synthetic anion transporters are developed to transport anions across lipid membranes with the long‐term perspective of biological applications. The lucigenin assay is a popular tool to study their transport of chloride and other anions in liposomes. It relies on the quenching of the fluorescence of encapsulated lucigenin by anions, which can be monitored by fluorescence spectroscopy. This article provides a tutorial introduction to the practical use and understanding of the lucigenin assay. It describes in detail how to use this assay to monitor chloride/nitrate antiport in liposomes, process and interpret the data, and solve common issues. Variations of the assay enabling the investigation of the transport of other anions and transport mechanisms are discussed. Furthermore, a zwitterionic analogue of lucigenin is demonstrated to have advantages for use in experiments over longer time scales, as it does not leak out of the liposomes, or when studying chloride uniport, as it avoids interference from antiport with nitrate that is present in commercial lucigenin.

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