Chemiluminescence strategy induced by HRP-sandwich structure based on strand displacement for sensitive detection of DNA methyltransferase

Yan, Xiaojun; Xue, Xinxin; Deng, Xiaojie; Jian, Yuting; Luo, Jing; Jiang, Mengmeng; Zheng, Xiangjuan

doi:10.1016/j.microc.2020.105183

Cited by 9 publications

(4 citation statements)

References 44 publications

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“…As shown in Figure , the relative activity of Dam MTase decreased remarkably as concentration of 5-fluorouracil augmented. The IC 50 value, 50% inhibitory concentration, was calculated to be 0.93 μM, which is comparable to the reported values. , The result indicated our assay could be utilized to screen potential Dam MTase inhibitors.…”

Section: Resultssupporting

confidence: 84%

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Cyclometalated Iridium(III) Complex-Sensitized NiO-Based-Cathodic Photoelectrochemical Platform for DNA Methyltransferase Assay

Cai

Zhang

Wang

et al. 2021

ACS Appl. Bio Mater.

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This work reports an efficient [(C6) 2 Ir(dppz)] + PF 6 − (C6 = coumarin 6 and dppz = dipyridophenazine)-sensitized NiO photocathode and its application in photoelectrochemical (PEC) bioanalysis field for the first time. This dye-sensitized NiO photocathode was found to exhibit a markedly enhanced cathodic photocurrent. A sensitive cathodic PEC platform was proposed integrating the as-prepared photocathode with enzyme-free cascaded amplification strategies of the catalytic hairpin assembly (CHA) and the hybridization chain reaction (HCR) for DNA methyltransferase (MTase) assay. A hairpin DNA(H Dam ) with specific recognition site of Dam MTase in its stem was designed. The site of H Dam was methylated in the presence of Dam MTase and then cut by endonuclease DpnI. The released loop fragment, as an initiator, triggered the CHA circuit and the follow-up HCR circuit, resulting in long dsDNA concatemers on the ITO electrode. Numerous [(C6) 2 Ir(dppz)] + PF 6− were intercalated into dsDNA, and highly efficient signal amplification was realized. Benefiting from the superior iridium(III) complex-sensitized NiO photocathode and effective amplification strategy, a detection limit of 0.0028 U/mL for the determination of Dam MTase was achieved. Moreover, this work further demonstrated that these proposed tactics could be applied to screen Dam MTase activity inhibitors.

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Section: Resultssupporting

confidence: 84%

“…The IC 50 value, 50% inhibitory concentration, was calculated to be 0.93 μM, which is comparable to the reported values. 71,72 The result indicated our assay could be utilized to screen potential Dam MTase inhibitors.…”

Section: ■ Materials and Methodsmentioning

confidence: 88%

Cyclometalated Iridium(III) Complex-Sensitized NiO-Based-Cathodic Photoelectrochemical Platform for DNA Methyltransferase Assay

Cai

Zhang

Wang

et al. 2021

ACS Appl. Bio Mater.

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“…5 The dynamic properties of DTMSD reactions strictly adhere to the Watson-Crick base pairing principle; 6 thus, the dynamic behavior of DTMSD is predictable and controllable. DTMSD is highly programable and can be cascaded to implement many sophisticated functions, such as logical computation, [7][8][9][10][11][12] analog computation, [13][14][15][16] biological sensors [17][18][19] and molecular walkers. [20][21][22] DTMSD circuits can be categorized as digital synthetic biological circuits and analog synthetic biological circuits.…”

Section: Introductionmentioning

confidence: 99%

A nonlinear neural network based on an analog DNA toehold mediated strand displacement reaction circuit

et al. 2022

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“…In view of this, many methods have been developed to detect DNA MTase activity, such as colorimetric methods with double-stranded DNA (dsDNA) probes [ 7 ], electrochemistry with dsDNA probes and hairpin probes [ 8 , 9 , 10 ], fluorescence using dsDNA probes [ 11 , 12 , 13 ], hairpin probes and dumbbell probes [ 14 , 15 , 16 ], chemiluminescent immunoassays with dsDNA probes [ 17 ] and dumbbell probes [ 17 , 18 , 19 , 20 ], and surface-enhanced Raman scattering (SERS) using dsDNA probes [ 21 ] and hairpin probes [ 22 ]. In addition, these probes usually combine with other nucleotide probes to amplify signals, such as hybridization chain reaction (HCR) [ 23 , 24 ], strand displacement amplification(SDA) [ 25 ], and rolling circle amplification (RCA) [ 18 ], to detect DNA MTase activity precisely.…”

Section: Introductionmentioning

confidence: 99%

Simple Detection of DNA Methyltransferase with an Integrated Padlock Probe

Wang

Han

Zhou³

et al. 2022

Biosensors

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DNA methyltransferases (MTases) can be regarded as biomarkers, as demonstrated by many studies on genetic diseases. Many researchers have developed biosensors to detect the activity of DNA MTases, and nucleic acid amplification, which need other probe assistance, is often used to improve the sensitivity of DNA MTases. However, there is no integrated probe that incorporates substrates and template and primer for detecting DNA MTases activity. Herein, we first designed a padlock probe (PP) to detect DNA MTases, which combines target detection with rolling circle amplification (RCA) without purification or other probe assistance. As the substrate of MTase, the PP was methylated and defended against HpaII, lambda exonuclease, and ExoI cleavage, as well as digestion, by adding MTase and the undestroyed PP started RCA. Thus, the fluorescent signal was capable of being rapidly detected after adding SYBRTM Gold to the RCA products. This method has a detection limit of approximately 0.0404 U/mL, and the linear range was 0.5–110 U/mL for M.SssI. Moreover, complex biological environment assays present prospects for possible application in intricacy environments. In addition, the designed detection system can also screen drugs or inhibitors for MTases.

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Chemiluminescence strategy induced by HRP-sandwich structure based on strand displacement for sensitive detection of DNA methyltransferase

Cited by 9 publications

References 44 publications

Cyclometalated Iridium(III) Complex-Sensitized NiO-Based-Cathodic Photoelectrochemical Platform for DNA Methyltransferase Assay

Cyclometalated Iridium(III) Complex-Sensitized NiO-Based-Cathodic Photoelectrochemical Platform for DNA Methyltransferase Assay

A nonlinear neural network based on an analog DNA toehold mediated strand displacement reaction circuit

Simple Detection of DNA Methyltransferase with an Integrated Padlock Probe

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