Chemiluminescence of β-lactam antibiotics following oxidation by potassium Superoxide

Sun, Jingshun; Perrin, John H.

doi:10.1016/s0003-2670(96)00450-3

Cited by 14 publications

(8 citation statements)

References 4 publications

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“…12,13 Recently, several CL methods for the determination of penicillin antibiotics have been reported. [14][15][16][17][18][19][20][21][22] These were based on enhanced CL methods using luminol-hydrogen peroxide, 14 enhancing effects on the luminol CL systems, 15,20,21 quenching CL methods using the luminol CL systems, 16,17,22 and direct CL methods based on potassium superoxide oxidation 18 or potassium permanganate oxidation. 19 Moreover, the nature of the reaction mechanism also has been demonstrated in detail.…”

Section: Introductionmentioning

confidence: 99%

“…15 In this work, a new flow-injection CL method is presented for the determination of mezlocillin which is based on the enhancing effect of mezlocillin on the CL emission generated by the oxidation of luminol with postassium periodate (KIO 4 ) in alkaline condition. As far as the authors know, although the CL behaviour of other penicillins has been reported, [12][13][14][15][16][17][18][19][20][21][22] this is the first use of the luminol-KIO 4 reaction for the determination of mezlocillin. Compared with previous CL assays for the determination of penicillin, [12][13][14][15][16][17][18][19][20][21][22] this method has higher sensitivity (the detection limit is 3.0 ng mL -1 mezlocillin) than previously used oxidants, such as hydrogen peroxide, 12 potassium ferricyanide, 13 iodine, 14,15 superoxide 16 or potassium permanganate.…”

Section: Introductionmentioning

confidence: 99%

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Chemiluminescence determination of mezlocillin by the luminol-potassium periodate system

Shi¹,

Yang²

2008

J. Braz. Chem. Soc.

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Um método novo, simples e sensível foi proposto para a determinação de mezlocilina. Baseia-se na amplificação da emissão de quimiluminescência (CL) gerada a partir da oxidação do luminol em meio alcalino pelo periodato de potássio. A otimização das variáveis experimentais e instrumentais que afetam o efeito da amplificação de CL foi realizada usando-se sistema de injeção em fluxo. Em condições ótimas, o método é eficiente para determinar mezlocilina no intervalo linear de 0,01 a 30 ×10-6 g mL -1 com limite de detecção (3σ) de 3,0×10 -9 g mL -1 e desvio padrão relativo (RSD) de 1,0% para 1,0×10-6 g mL -1 de mezlocilina (n=11). O método tem sido aplicado com sucesso, para determinação de mezlocilina em preparações comerciais, amostras sintéticas e formulações biológicas fluidas.A new, simple and sensitive method has been proposed for the determination of mezlocillin. It is based on the enhancement of the chemiluminescence (CL) emission generated from the oxidation of luminol in alkaline medium by postassium periodate. The optimization of the experimental and instrumental variables affecting the CL enhancement effect has been carried out using flow-injection system. In the optimum conditions, the method is efficient to determine mezlocillin in the linear range of 0.01-30×10-6 g mL -1 with a detection limit (3σ) of 3.0×10 -9 g mL -1and the relative standard deviation (RSD) is 1.0% for 1.0×10 -6 g mL -1 mezlocillin (n=11). It has been successfully applied to the mezlocillin determination in commercial preparations, synthetic samples and biological fluids formulations.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Chemiluminescence determination of mezlocillin by the luminol-potassium periodate system

Shi¹,

Yang²

2008

J. Braz. Chem. Soc.

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“…However, they often suffer from a variety of limitations. But analytical methods applying chemiluminescence (CL) [16][17][18][19][20][21][22][23][24][25][26][27] provide many advantages for determining pharmaceuticals such as high sensitivity, high selectivity, small amount of chemical consumption, instrumental simplicity (no monochromator required) and speed of in signal detection (∼0.1-10 s). Since many CL reactions are very fast, they give rise to imprecise measurements as a result of irreproducible mixing of sample and reagents, but the reproducibility and selectivity of CL analysis can be improved by combination with flow injection analysis (FIA).…”

Section: Introductionmentioning

confidence: 99%

Flow injection chemiluminescence determination of cefadroxil using potassium permanganate and formaldehyde system

Thongpoon

Liawruangrath

et al. 2006

Journal of Pharmaceutical and Biomedical Analysis

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“…Jingshun et al described a CL method for the determination of penicillin G with a linear range of 0.01 -0.1 mg/ml and dicloxacillin with a linear range of 0.2 -1.0 mg/ml. 12 It was based on the CL reaction of penicillins with potassium superoxide dissolved by 18-crown-6-ether. Kubo et al described a flow-injection CL method for the determination of β-lactam antibiotics, which was based on CL directly produced by the reaction of luminol with β-lactam antibiotics in the presence of a catalyst in an alkaline solution.…”

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confidence: 99%

Flow Injection Chemiluminescence Analysis of Some Penicillins by Their Sensitizing Effect on the Potassium Permanganate-Glyoxal Reaction

et al. 2005

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Penicillins, a kind of β-lactam antibiotics, are active on sensitive gram-positive and gram-negative organisms. Many analytical procedures have been reported for their determination. The Chinese Pharmacopoeia (2nd ed., 2000) recommends HPLC or titration assays for their dosages. 1 Other reported methods include: spectrophotometry, 2-4 mercurimetric titration, 5 NMR, 6 HPLC, 7-10 and HPLC-APCI-MS. 11There have been a few reports on chemiluminescence (CL) methods for the determination of penicillins. Jingshun et al. described a CL method for the determination of penicillin G with a linear range of 0.01 -0.1 mg/ml and dicloxacillin with a linear range of 0.2 -1.0 mg/ml. 12 It was based on the CL reaction of penicillins with potassium superoxide dissolved by 18-crown-6-ether. Kubo et al. described a flow-injection CL method for the determination of β-lactam antibiotics, which was based on CL directly produced by the reaction of luminol with β-lactam antibiotics in the presence of a catalyst in an alkaline solution. The method allowed the determination of penicillin G in linear range of 0.12 -6.0 mg/ml with a detection limit of 12 µg/ml. 13The present article describes a simple flow injection CL method for the determination of four penicillins: phenoxymethylpenicillin potassium, amoxicillin, ampicillin, and ampicillin sodium. The method is based on an enhancing effect of penicillins on the CL reaction of glyoxal with KMnO4 in sulfuric acid. The experimental conditions for the reaction were optimized, and the final procedure allowed a successful determination of the studied drugs in pharmaceutical preparations. Experimental Reagents and chemicalsAll of the reagents were of analytical reagent grade, and doubly distilled water was used to prepare all solutions. Phenoxymethylpenicillin potassium, amoxicillin, ampicillin, and ampicillin sodium standard samples were purchased from National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). KMnO4 was obtained from Xi'an Chemical Reagent Factory (Xi'an, China). Glyoxal was provided by Department of Chemistry of Xi'an Jiaotong University (Xi'an, China). The 1.0 mg/ml standard solutions of phenoxymethylpenicillin potassium, amoxicillin, ampicillin, and ampicillin sodium were freshly prepared by dissolving 250 mg of each in water and diluting with water to 250 ml. The working solutions were prepared by appropriate dilution of these standard solutions with water before being used. A KMnO4 solution 1.0 × 10 -3 mol/l was prepared by diluting an appropriate 0.1 mol/l stock solution in 1.3 mol/l H2SO4. A glyoxal solution (0.05 mol/l) was prepared from a 1 mol/l stock solution. InstrumentsThe flow system used for the determination and CL detection for the studied penicillins is shown schematically in Fig. 1. One peristaltic pump (two channels) was used to drive KMnO4 in sulfuric acid and glyoxal solutions. Another peristaltic pump was used to deliver the sample solution. Each pump was run at with a constant flow rate of 2.75 ml/min. PTFE tubing (0.8...

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Chemiluminescence of β-lactam antibiotics following oxidation by potassium Superoxide

Cited by 14 publications

References 4 publications

Chemiluminescence determination of mezlocillin by the luminol-potassium periodate system

Chemiluminescence determination of mezlocillin by the luminol-potassium periodate system

Flow injection chemiluminescence determination of cefadroxil using potassium permanganate and formaldehyde system

Flow Injection Chemiluminescence Analysis of Some Penicillins by Their Sensitizing Effect on the Potassium Permanganate-Glyoxal Reaction

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