Chemiluminescence of iron-chlorophyllin

Abstract

The iron-chlorophyllin complex was found to be chemiluminescent (CL) in an acetonitrile (22%)/water mixed solvent. In the presence of hydrogen peroxide, when iron-chlorophyllin was added to the mixed solvent, a sharp CL signal immediately appeared. Also, analysis of the absorption spectra revealed decomposition of iron-chlorophyllin (based on decrease in absorbance at 396 nm), hence iron-chlorophyllin is the CL substance. Moreover, the CL intensity decreased in the presence of potassium thiocyanate (KSCN), indicating that the axial coordinative position of iron-chlorophyllin acts as a point of catalytic activation. In addition, when fluorophores were present with iron-chlorophyllin CL, their CL intensity values were similar to or greater than that of the well-known trichlorophenylperoxalate ester (TCPO) CL. Thus, during the decomposition reaction of iron-chlorophyllin, the latter transfers its energy to the coexisting fluorophores. Moreover, since the decomposed compound in this CL reaction had a fluorescence, it was found that the iron-chlorophyllin also functions as an energy donor. Therefore, the iron-chlorophyllin complex acts not only as a CL substance, but also as a catalyst and energy donor in the reaction.

Quenching-Chemiluminescence Determination of Trace Amounts ofl-Tyrosine Contained in Dietary Supplement by Chemiluminescence Reaction of an Iron-Phthalocyanine Complex

“…Therefore, the appearance of a high CL substance is strongly desired. Up to now, the authors have reported about the chlorophyll CL [15] and the iron-chlorophyllin complex CL [16]. In these CLs, each compound itself has multifunctions such as a luminescence substrate, a catalyst, and an energy donor.…”

A 13-week subchronic toxicity study of sodium iron chlorophyllin in F344 rats

“…In addition, it has been demonstrated that sodium copper chlorophyllin, a food additive with similar structure to SIC, shows inhibitory effects on heterocyclic amineinduced mammary carcinogenesis in rats, possibly by the prevention of absorption of carcinogens in the gastrointestinal tract (Hasegawa et al, 1995;Hirose et al, 2002). Aside from the safety assessment, other studies have reported that SIC could be applied for proteomic analysis or chemiluminescence (Kato et al, 2005;Nagoshi et al, 2005;Zhang et al, 2012).…”