Chemiluminescence of human polymorphonuclear leucocytes after stimulation with whole cells and cell-wall components of Staphylococcus epidermidis

Martínez-Martínez, Laura; Timmerman, C. P.; Fleer, A.; Verhoef, J.

doi:10.1099/00222615-39-3-196

Cited by 17 publications

(10 citation statements)

References 22 publications

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“…Thus, a significant decrease in cytokine release was measured when adding neutrophils to LPS/ monocyte cultures. Interestingly, while MDP is the minimal active motif of peptidoglycans that can stimulate cytokine production in mononuclear cells, it does not stimulate either cytokine production [23] or metabolic activation [24] in neutrophils, while peptidoglycans are capable of eliciting these effects.…”

Section: Discussionmentioning

confidence: 99%

Modulation of muramyl dipeptide stimulation of cytokine production by blood components

Meer

Netea

Dinarello

2009

Clinical and Experimental Immunology

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SummaryMuramyl dipeptide (MDP) is the minimal active fragment of peptidoglycan of the cell wall of Gram-positive bacteria, with potential beneficial effects as a vaccine adjuvant. Peptidoglycans and MDP are recognized by the intracellular receptor NOD2 (nucleotide-binding oligomerization domain 2), leading to production of proinflammatory cytokines. In the present study, it is shown that, despite stimulatory effects on isolated human mononuclear cells, MDP does not stimulate production of tumour necrosis factor-a, interleukin-1b or interleukin-6 in a whole-blood assay. However, MDP retains synergistic effects on lipopolysaccharide-induced cytokines in whole blood. Screening tests of NOD2 function based on whole-blood stimulation should therefore employ strategies based on the synergistic effects of MDP on Toll-like receptorinduced cytokine production. Plasma was not responsible for the inhibition of MDP in whole blood. The inhibition of MDP stimulation was dependent upon cellular components, with erythrocyte-derived haemoglobin and neutrophils collaborating in the inhibition of MDP effects in whole blood.

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Section: Discussionmentioning

confidence: 99%

Modulation of muramyl dipeptide stimulation of cytokine production by blood components

Meer

Netea

Dinarello

2009

Clinical and Experimental Immunology

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“…Possible S. epidermidis molecular candidates for eliciting ROS production in monocytes stem from the work of Martínez-Martínez et al 50 showing that S. epidermidis cell wall peptidoglycan is the crucial CL-inducing component in human polymorphonuclear cells. The mechanism of CL response induced by S. epidermidis may involve the toll-like receptor 2 in the activation of the NF-κB, as demonstrated for peptidoglycan and lipoteichoic acid from gram-positive bacteria.…”

Section: Monocyte Activation In Response To Different Substrates and mentioning

confidence: 99%

Role of nanostructured gold surfaces on monocyte activation and Staphylococcus epidermidis biofilm formation

Svensson¹,

Forsberg²,

Hulander³

et al. 2014

IJN

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The role of material surface properties in the direct interaction with bacteria and the indirect route via host defense cells is not fully understood. Recently, it was suggested that nanostructured implant surfaces possess antimicrobial properties. In the current study, the adhesion and biofilm formation of Staphylococcus epidermidis and human monocyte adhesion and activation were studied separately and in coculture in different in vitro models using smooth gold and well-defined nanostructured gold surfaces. Two polystyrene surfaces were used as controls in the monocyte experiments. Fluorescent viability staining demonstrated a reduction in the viability of S. epidermidis close to the nanostructured gold surface, whereas the smooth gold correlated with more live biofilm. The results were supported by scanning electron microscopy observations, showing higher biofilm tower formations and more mature biofilms on smooth gold compared with nanostructured gold. Unstimulated monocytes on the different substrates demonstrated low activation, reduced gene expression of pro- and anti-inflammatory cytokines, and low cytokine secretion. In contrast, stimulation with opsonized zymosan or opsonized live S. epidermidis for 1 hour significantly increased the production of reactive oxygen species, the gene expression of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, and IL-10, as well as the secretion of TNF-α, demonstrating the ability of the cells to elicit a response and actively phagocytose prey. In addition, cells cultured on the smooth gold and the nanostructured gold displayed a different adhesion pattern and a more rapid oxidative burst than those cultured on polystyrene upon stimulation. We conclude that S. epidermidis decreased its viability initially when adhering to nanostructured surfaces compared with smooth gold surfaces, especially in the bacterial cell layers closest to the surface. In contrast, material surface properties neither strongly promoted nor attenuated the activity of monocytes when exposed to zymosan particles or S. epidermidis .

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“…These structures in the CW complex, or alone, are biologically active, with immunoadjuvant or slow wave sleep promoting activity. 2 During Gram positive infections, PG can activate complement 3 and granulocytes, 4 and up regulate expression of adhesion molecules on the endothelial cells. 4 In vitro, PG is known to induce production of the proinflammatory cytokines interleukin 1, interleukin 6, tumour necrosis factor α (TNFα), and monocyte chemoattractant protein-1 (MCP-1) by monocytes.…”

mentioning

confidence: 99%

“…2 During Gram positive infections, PG can activate complement 3 and granulocytes, 4 and up regulate expression of adhesion molecules on the endothelial cells. 4 In vitro, PG is known to induce production of the proinflammatory cytokines interleukin 1, interleukin 6, tumour necrosis factor α (TNFα), and monocyte chemoattractant protein-1 (MCP-1) by monocytes. [5][6][7][8] Bacterial CWs are digested by a number of PG degrading hydrolytic enzymes, including lysozyme, and the amounts of active CW fragments released depend on the activity of these PG hydrolases.…”

mentioning

confidence: 99%

Role of peptidoglycan subtypes in the pathogenesis of bacterial cell wall arthritis

Šimelyte

Rimpiläinen

Zhang

et al. 2003

Annals of the Rheumatic Diseases

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Background: Bacterial cell wall (CW) arthritis develops in susceptible strains of rats after a single intraperitoneal injection of the CW from certain bacterial species, both pathogenic and non-pathogenic. For the development of chronic bacterial CW arthritis, the structure of the bacterial peptidoglycan (PG) has been found to be decisive. Objective: To define the role of PG subtypes in the pathogenesis of chronic bacterial CW arthritis. Method: Arthritis was induced with CWs of Lactobacillus plantarum, L casei B, L casei C, and L fermentum. Gas chromatography-mass spectrometry was used to measure the presence of CW derived muramic acid in the liver and to determine PG subtypes. CWs were also tested for their resistance to lysozyme in vitro. Results: These results and those published previously indicate that PGs of CWs which induce chronic arthritis, no matter whether they were derived from strains of Streptococcus, Bifidobacterium, Collinsella, or Lactobacillus, all have lysine as the third amino acid of the PG stem peptide, representing PG subtypes A3α and A4α. Those strains which induce only transient acute arthritis or no arthritis at all do not have lysine in this position, resulting in different PG subtypes. Conclusions: In vivo degradation of only those PGs with the subtypes A3α and A4α leads to the occurrence of large CW fragments, which persist in tissue and have good proinflammatory ability. CWs with other PG subtypes, even if they are lysozyme resistant, do not cause chronic arthritis, because the released fragments are not phlogistic. It is emphasised that a variety of microbial components not causing inflammation have been found in animal and human synovial tissue.

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Chemiluminescence of human polymorphonuclear leucocytes after stimulation with whole cells and cell-wall components of Staphylococcus epidermidis

Cited by 17 publications

References 22 publications

Modulation of muramyl dipeptide stimulation of cytokine production by blood components

Modulation of muramyl dipeptide stimulation of cytokine production by blood components

Role of nanostructured gold surfaces on monocyte activation and Staphylococcus epidermidis biofilm formation

Role of peptidoglycan subtypes in the pathogenesis of bacterial cell wall arthritis

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