Chemiluminescence microfluidic system of gold nanoparticles enhanced luminol-silver nitrate for the determination of vitamin B12

Abstract

A rapid and sensitive chemiluminescence (CL) system coupled with a microfluidic chip has been presented to determine vitamin B12 (VB12) based on the reaction of luminol and silver nitrate (AgNO(3)) in the presence of gold nanoparticles (AuNPs). A microfluidic chip was fabricated by a soft-lithographic procedure using polydimethyl siloxane (PDMS) having four inlets and one outlet with a 200 μm wide, 250 μm deep, and 100 mm long microchannel. Ag(+) was used as a chemiluminogenic oxidant in this CL reaction which oxidized luminol to produce strong CL signal in the presence of AuNPs. Luminol reacted with AgNO(3) under the catalysis of AuNPs to produce luminol radicals which reacted with dissolved oxygen and emitted CL light. The proposed CL system was applied to determine the amount of VB12 in VB12 tablets and multivitamin. Under the optimum conditions, the CL intensity of the system was increased with the concentration of VB12 in the range of 0.25-100 ng mL(-1) with the correlation coefficient of 0.9982. The limit of detection was found to be 0.04 ng mL(-1) with the relative standard deviation of 1.56 % for five replicate determinations of 25 ng mL(-1) of VB12. The CL reaction mechanism was demonstrated by UV-visible spectra and CL emission spectra.

Luminol-Based Chemiluminescent Signals: Clinical and Non-clinical Application and Future Uses

“…Luminol reacts with silver nitrate by using gold nanoparticles as a catalyst to produce luminol radicals, which react with dissolved oxygen and emit CL light. This CL method was used to determine the amount of vitamin B 12 in tablets and multivitamin preparations [174]. …”

Design a New Strategy Based on Nanoparticle-Enhanced Chemiluminescence Sensor Array for Biothiols Discrimination

“…S2, AgNO 3 was reduced to Ag atoms and luminol was oxidized to luminol radical which were catalyzed by gold nanoparticles and happened on the surface of gold nanoparticles. Luminol radical was then followed by reaction with the dissolved oxygen, giving rise to light emission12394041. The possible mechanism of the enhanced luminol–H 2 O 2 CL reaction induced by CdTe QDs was shown in Fig.…”

Microfluidic opportunities in the field of nutrition

“…120 Recently, microfluidic chemiluminescence systems for measurements of vitamin B1 and B12 levels in tablets or eggs have also been reported, with very high sensitivity compared to reported methods. 121–123 Besides system miniaturization, another distinct merit of microfluidics technology is the ease of parallelization, which can be realized simply by introducing multiple channels. This can increase throughput or enable multiplexed analysis.…”