Chemiluminescence-linked immunoassay for detection of mumps virus antibodies

Abstract

We have developed a simple and sensitive chemiluminescence-linked immunoassay (CLIA) for determining mumps virus antibodies. Luminol molecules were used as markers, and polystyrene balls were used as antigen carriers. The CLIA was compared with an enzyme-linked fluorescence assay and a hemagglutination inhibition test on a total of 40 serum specimens obtained from 29 donors with natural infection or vaccination. There was good correlation between the three methods, and the sensitivity of the CLIA was about 10 times higher than that of the hemagglutination inhibition test, although it was slightly inferior to that of the enzyme-linked fluorescence assay. Moreover, the time course of light emission from the labeled antibody was rapid, and therefore in the CLIA the quantitation of the marker takes only a short time.

Comparison of colorimetric, fluorescent, and enzymatic amplification substrate systems in an enzyme immunoassay for detection of DNA-RNA hybrids

“…Efficient enzymatic labels generating products, such as fluorescent (3, 4, 7-10, 12, 14, 25, 35), chemiluminescent (15), or radioactive (11) compounds, that are measurable at lower concentrations than visibly colored products could provide an alternative to increase the sensitivity of EIA. Enzymatically amplified cycling reaction represents another method of magnifying the signal produced by enzymatic reactions (16,17,19,28).…”

Production and use of monoclonal antibodies against the potato virus Y group

“…Other improvements in the development of immunoassay technology have been described, and they include the use of fluorescent substrates (85,148), chemiluminescent labels (129), radioactive substrates (59) , and biotin-avidin linked systems. The improvement of sensitivity and speci ficity through the use of monoclonal antibodies has also been investigated.…”