Chemiluminescence in vitamin E-deficient erythrocytes initiated by xanthine oxidase reaction, in relation to the accumulation of thiobarbituric acid reactive substances.

The concentration-dependent effects of copper acting either as an antioxidant or as a prooxidant were examined in vitro using Tween 20-induced hemolysis. When cupric ion concentration was more than 10 microM, free copper(II) acted as a prooxidant; both extensive hemolysis and production of unknown thiobarbituric acid-reactive substance occurred in hamster and pig erythrocytes irrespective of vitamin E status. However, when cupric ion concentration was 2-4 microM in the incubation medium, copper showed a clear antioxidant activity, reducing both hemolysis and malondialdehyde production induced either by diluted peroxide-containing Tween 20 with ascorbic acid and sodium azide in vitamin E-deficient hamster erythrocytes, or by peroxide-containing Tween 20 in pig erythrocytes containing marginal amounts of vitamin E. Copper(II) is taken up by the erythrocytes, where copper(I)-complexes may contribute to the protection of cells with membrane vitamin E against oxidative radical attack.

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Changes in membrane constituents and chemiluminescence in vitamin E-deficient red blood cells induced by the xanthine oxidase reaction

Yasuda

Miki

Takenaka

et al. 1989

Archives of Biochemistry and Biophysics

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Chemiluminescence in vitamin E-deficient erythrocytes initiated by xanthine oxidase reaction, in relation to the accumulation of thiobarbituric acid reactive substances.

Cited by 5 publications

References 16 publications

Chemiluminescence from Vitamin E-Deficient Erythrocyte Membranes Induced by Xanthine Oxidase Reaction

Chemiluminescence from Vitamin E-Deficient Erythrocyte Membranes Induced by Xanthine Oxidase Reaction

Antioxidant and prooxidant roles of copper in Tween 20-induced hemolysis of hamster and pig erythrocytes containing marginal vitamin E

Changes in membrane constituents and chemiluminescence in vitamin E-deficient red blood cells induced by the xanthine oxidase reaction

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