Chemiluminescence in liquid scintillation counting

Woods, Anna; O'Bar, P.R.

doi:10.1016/0003-2697(70)90359-3

Cited by 8 publications

(1 citation statement)

References 4 publications

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“…The conversion of counts per minute (cpm) to disintegrations per minute (dpm) is subject to artifacts such as uneven quenching of the scintillation, instrument drift, variability of automatic quench corrections, and chemiluminescence (59,62,74). At present, very few investigators use internal standardization that involves (a) multiple counting of samples until stabilization of the cpm, (b) careful pipetting of an internal standard of [ 14 C]-or [ 3 H]toluene into the scintillation vial, (c) multiple recounting of the samples, (d ) computing the counting efficiency of each sample, and (e) using the counting efficiency to convert cpm to dpm (19,22,26).…”

Section: Simulation Of the Impact Of Measurement Errors On Rates Of Gmentioning

confidence: 99%

Is There Glucose Production Outside of the Liver and Kidney?

Previs

Brunengraber

2009

Annu. Rev. Nutr.

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This review analyzes the evidence presented to support the role of organs other than the liver and kidney to release substantial amounts of glucose into the mammalian blood circulation. The evidence includes (a) the identification of gluconeogenic enzyme activities in various organs, especially the small intestine, (b) levels of mRNA for the same enzymes, and (c) measurements of gluconeogenic flux in the small intestine. The latter would be the definite proof of extrahepatic, extrarenal glucose production. We critically evaluate the radioactive and stable isotopic techniques used to measure intestinal gluconeogenesis. We also simulate the impact of unavoidable measurement errors on apparent rates of intestinal gluconeogenesis. We conclude that there is so far no credible evidence to support the concept that glucose can be produced by the intestine or by muscle.

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