Chemiluminescence immunoassay: an overview

Weeks, Ian; Sturgess, M.; Woodhead, J. S.

doi:10.1042/cs0700403

Cited by 66 publications

(19 citation statements)

References 5 publications

(5 reference statements)

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“…The CLIA reported here was designed for assaying plasma MTP-PE in clinical trial studies in the sensitivity range required for pharmacokinetic studies in man. The potential of CL molecules as non-isotopic labels in immunoassay has been well established (Weeks et al, 1983(Weeks et al, , 1984(Weeks et al, , 1986Weeks and Woodhead, 1988). This paper describes a simple competitive CLIA for MTP-PE using an acridinium ester-labelled analogue of MTP as a tracer (Fig.…”

Section: Introductionmentioning

confidence: 98%

Direct chemiluminescence immunoassay (CLIA) for muramyl tripeptide phosphatidyl‐ethanolamine in plasma

Towbin

Schnell

Einsle

et al. 1991

J. Biolumin. Chemilumin.

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A competitive chemiluminescent immunoassay for quantitation of muramyl tripeptide phosphatidyl-ethanolamine (MTP-PE) in plasma has been developed. The assay is based on the use of an acridinium ester-labelled analogue of muramyl tripeptide and a rabbit antiserum. It includes an overnight incubation and a separation with a second antibody covalently coupled to paramagnetic particles. The sensitivity of detection is 0.012 nmol/l, the assay working range is 0.1-5 nmol/l, and the inter-assay CVs are less than or equal to 10%. Using up to 6000-fold sample dilutions, a wide working range (0.1-30,000 nmol/l) is obtained. Rat plasma samples were collected during and one day after intravenous infusion of MTP-PE. Following infusion, the concentrations in plasma declined multiphasically. Half-life time was 0.37 h +/- 0.03 (mean +/- SD, alpha phase) and 1.76 h +/- 0.08 (mean +/- SD, beta phase), clearance and volume of distribution were 0.09 +/- 0.02 l/h x kg (mean +/- SD) and 0.06 +/- 0.01 l/kg (mean +/- SD) respectively. The use of an acridinium ester as a chemiluminescent (CL) label overcomes the problems associated with reagents of limited shelf-life.

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Section: Introductionmentioning

confidence: 98%

Direct chemiluminescence immunoassay (CLIA) for muramyl tripeptide phosphatidyl‐ethanolamine in plasma

Towbin

Schnell

Einsle

et al. 1991

J. Biolumin. Chemilumin.

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“…One employs acridinium esters in twosite immunochemiluminometric assays (ICMA) (1). ICMA has been used to measure thyrotropin (TSH) (2,3), alpha-fetoprotein (4,5), and other analytes.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of enhanced luminescence immunoenzymometric assays (lia) for ferritin and free T4

Armbruster¹,

Jirinzu²,

Williams³

1989

Clinical Laboratory Analysis

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The Amerlite enhanced luminescence immunoenzymometric assays (LIA) for ferritin and free T4 (FT4) have been evaluated. The LIA assay system employs a two-site binding approach in which one antibody is coated on the interior of plastic microtiter wells and another antibody, or antigen, is tagged with horseradish peroxidase (HRPO). Signal, proportional to the amount of patient analyte, is produced when the HRPO catalyzes the oxidation of luminol resulting in light production. The light signal is increased in magnitude by the incorporation of an enhancing agent which also greatly prolongs the lifetime of the signal. Light production is monitored by an automated luminometer which uses a stored calibration curve to calculate patient results. FT4 within-run coefficients of variation (CVs) ranged from 2.7% to 6.6%; day-to-day CVs from 6% to 15.5% for three levels of control. Sensitivity is 0.03 ng/dl. T3 concentrations up to 8 ng/ml produced no cross-reactivity. Dilutional linearity was exhibited. Lipemia and icterus produced positive interferences. Linear regression analysis of Amerlite and radioimmunoassay (RIA) FT4 values yielded the following values: Amerlite = 0.63 RIA - 0.2, r = .87, n = 79. Ferritin within-run CVs ranged from 4.9% to 10.5%; day-to-day CVs from 5.8-12.5% for three levels of control. Sensitivity is less than 1 ng/ml. Recoveries ranged from 93% to 106% (av. = 99.4%). Dilutional linearity was exhibited. Lipemia caused a positive interference; hemoglobin caused a negative interference. Linear regression analysis of Amerlite and RIA ferritin values yielded the following values: Amerlite = 1.0 RIA + 11.1, r = .96, n = 55.

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“…The Chemiluminescent Immuno Assay (CIA) (Weeks,1986;Sturgess, and Woodhead, 1986;Wood, 1985) offers a convenient and accurate method that is often more rapid than the ELISA. Instruments for this type of assay, using electrochemiluminescence reactions, are in development (The Origen analyzer, IGEN Inc., Rockville, Maryland).…”

Section: Introductionmentioning

confidence: 99%

Comparison of an electrochemiluminescent homogenous immunoassay and an ELISA for monitoring productivity in mammalian cell bioreactors

Lindell

Hall²,

Wu³

et al. 1991

Biotechnol Tech

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Chemiluminescence immunoassay: an overview

Cited by 66 publications

References 5 publications

Direct chemiluminescence immunoassay (CLIA) for muramyl tripeptide phosphatidyl‐ethanolamine in plasma

Direct chemiluminescence immunoassay (CLIA) for muramyl tripeptide phosphatidyl‐ethanolamine in plasma

Evaluation of enhanced luminescence immunoenzymometric assays (lia) for ferritin and free T4

Comparison of an electrochemiluminescent homogenous immunoassay and an ELISA for monitoring productivity in mammalian cell bioreactors

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