Chemiluminescence Flow System for Vanadium(V) With Immobilized Reagents

Abstract

A chemiluminescence (CL)-based system for vanadium(v) combined with flow-injection analysis is described. The analytical regents, luminol and hexacyanoferrate(II), were both immobilized on an anion-exchange resin column. When a volume of phosphoric acid was passed through the column, these two reagents were eluted from the resin and then mixed with a vanadium(v) stream under acidic conditions. By means of the fast oxidation reaction between vanadium(v) and hexacyanoferrate(II), vanadium(IV) and hexacyanoferrate (III) were generated, both of which catalyzed the oxidation of luminol by dissolved oxygen in aqueous alkaline solution to produce CL. The CL emission intensity was correlated with the standard vanadium (v) concentration in the range from 1.0 x 10(-2) to 10 micrograms cm-3, and the detection limit was 5.4 x 10(-3) micrograms cm-3 vanadium (v). Interfering metal ions co-existing in sample solutions could be effectively separated on-line by a cation-exchange column placed upstream. A complete analysis, including sampling and washing, could be performed in 1 min with a relative standard deviation of less than 5%. The system was stable for over 100 analyses and was applied successfully to the determination of vanadium in geochemical and human hair samples.

Kinetic study of site directed and randomly immobilized his‐tag alkaline phosphatase by flow injection chemiluminescence

“…Chemiluminescence (CL) coupled with flow injection analysis (CLFIA) has many analytical applications such as: the determination of metal ions, hydrogen peroxide, alcohols, amines, amino acids, carbohydrates, drugs, disease marker, enzymes, vitamins and other inorganic and organic species (Bowie et al, 1996;Qin et al, 1997;Li et al, 2003;Lin et al, 2004;Messina et al, 2005).…”

A sensitive method for determining total vanadium in water samples using colorimetric-solid-phase extraction-fiber optic reflectance spectroscopy

“…The direct analysis of analyte on the surface of adsorbent-matrix is convenient because of its simplicity and rapidity for routine analyses. The publications dealing with optical vanadium sensing have increased recently [8][9][10]. Immobilised Alizarin Red S [8], luminol [9] and fatty hydroxamic acid [10] in various matrices were used for vanadium sensing based on fluorescence [8], chemiluminescence [9] reflectance [10] and electrochemical measurements [11][12][13][14][15][16].…”

A chemiluminescence flow injection system for nitrite ion determination

“…Chemiluminescence, as one of the most sensitive detection methods, offers a wide linear range, low detection limit, simple and inexpensive instrumentation and very short analysis time [20]. There are numerous reports published regarding the importance of chemiluminescence methods [21][22][23][24] and their application in the determination of a wide variety of compounds including organic [25][26][27], inorganic [28][29][30], biological [31][32][33][34][35], and pharmaceutical [36][37][38][39][40][41] compounds in different samples.…”