Chemiluminescence Enzyme Immunoassay Using Bacterial Magnetic Particles

Matsunaga, Tadashi; Kawasaki, Masashi; Xiao, Yu; Tsujimura, Noriyuki; Nakamura, Noriyuki

doi:10.1021/ac9603690

Cited by 111 publications

(68 citation statements)

References 17 publications

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“…glutaric dialdehyde). These magnetic particles have been applied as active components in fluoroimmunoassays, [315] chemiluminescence immunoassays, [316] mRNA recovery, [317] and as DNA carriers. [318] 7.2.…”

Section: Methodsmentioning

confidence: 99%

Integrated Nanoparticle–Biomolecule Hybrid Systems: Synthesis, Properties, and Applications

2004

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Nanomaterials, such as metal or semiconductor nanoparticles and nanorods, exhibit similar dimensions to those of biomolecules, such as proteins (enzymes, antigens, antibodies) or DNA. The integration of nanoparticles, which exhibit unique electronic, photonic, and catalytic properties, with biomaterials, which display unique recognition, catalytic, and inhibition properties, yields novel hybrid nanobiomaterials of synergetic properties and functions. This review describes recent advances in the synthesis of biomolecule-nanoparticle/nanorod hybrid systems and the application of such assemblies in the generation of 2D and 3D ordered structures in solutions and on surfaces. Particular emphasis is directed to the use of biomolecule-nanoparticle (metallic or semiconductive) assemblies for bioanalytical applications and for the fabrication of bioelectronic devices.

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Section: Methodsmentioning

confidence: 99%

Integrated Nanoparticle–Biomolecule Hybrid Systems: Synthesis, Properties, and Applications

2004

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“…Two heterofunctional cross linkers were used for the preservation of antibody specificity after chemical conjugation. Sulphosuccinimidyl 4-(N-maleimidomethyl)-cyclohexane-1-carboxylate, which contains the NHS ester and maleimide, was reacted with antibodies (Matsunaga et al 1996a). Streptavidin immobilized on BacMPs was prepared using the same principle.…”

Section: Protein Assembly On the Surface Of Magnetic Particlesmentioning

confidence: 99%

Formation of magnetite by bacteria and its application

Arakaki

Nakazawa

Nemoto

et al. 2008

J. R. Soc. Interface.

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220

176

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Magnetic particles offer high technological potential since they can be conveniently collected with an external magnetic field. Magnetotactic bacteria synthesize bacterial magnetic particles (BacMPs) with well-controlled size and morphology. BacMPs are individually covered with thin organic membrane, which confers high and even dispersion in aqueous solutions compared with artificial magnetites, making them ideal biotechnological materials. Recent molecular studies including genome sequence, mutagenesis, gene expression and proteome analyses indicated a number of genes and proteins which play important roles for BacMP biomineralization. Some of the genes and proteins identified from these studies have allowed us to express functional proteins efficiently onto BacMPs, through genetic engineering, permitting the preservation of the protein activity, leading to a simple preparation of functional protein-magnetic particle complexes. They were applicable to high-sensitivity immunoassay, drug screening and cell separation. Furthermore, fully automated single nucleotide polymorphism discrimination and DNA recovery systems have been developed to use these functionalized BacMPs. The nano-sized fine magnetic particles offer vast potential in new nano-techniques.

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“…[23] Then, the magnetic nanocrystals were separated by using a magnet (Nd±B) placed at the bottom of the glass tube containing the disrupted cells. [24] After the supernatant was removed, the collected magnetic nanocrystals were washed ten times with 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethane sulfonic acid (HEPES) buffer (pH 7.2). The purified nanocrystals were resuspended in HEPES (1 mg mL ±1…”

mentioning

confidence: 99%