Chemiluminescence determination of vitamin B12 by a flow-injection method

Zhou, Yikai; Li, H.; Liu, Y.; Liang, Guoxi

doi:10.1016/s0003-2670(00)82550-7

Cited by 37 publications

(20 citation statements)

References 4 publications

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“…29 In the determination of vitamin B 12 (cyanocobalamin), the analyte is acidified to release Co(II), which catalyses the reaction of luminol. 30,31 In the determination of vitamin B 6 (pyridoxine), the analyte acts as a sensitizer for the luminol reaction. 32 Determinations of molecules containing the thiol group exploit their inhibitory effect on the chemiluminescence produced in the oxidation of luminol by hypochlorite ion through reduction of the oxidant.…”

Section: ààààà3mentioning

confidence: 99%

Flow‐chemiluminescence: a growing modality of pharmaceutical analysis

2001

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Section: ààààà3mentioning

confidence: 99%

Flow‐chemiluminescence: a growing modality of pharmaceutical analysis

2001

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“…In the literature, several methods including microbiological assay [3], spectrophotometry [4], fluorimetry [5], chemiluminescence [6], atomic absorption spectrometry [7], capillary electrophoresis [8] and high-performance liquid chromatography (HPLC) [9 12] have been proposed for the determination of vitamins. Several reviews about determination of vitamins have also been published [13 16].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of twelve water- and fat-soluble vitamins by high-performance liquid chromatography with diode array detection

Chen

2001

Chromatographia

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SummaryA novel method for the simultaneous determination of Iwelve water-and fat-soluble vitamins has been established by high-performance liquid chromatography with diode array detection. The vitamins were analyzed on a I~Bondapak Cls column (300 x 3.9 mm, 10 I~m) with methanol-KH2P04 buffer (0.1 M, pH 7.0)-water as mobile phase in a gradient. The hnearity of calibration graphs was compound-dependent and the detection limits ranged from 0.02 I~g mL 1 to 0.5 I~g mL 1. The method was successfully applied to determine vitamins in pharmaceutical preparations. The recoveries were from 95.1% to 103% and the relative standard deviations were in the range of 0.9% to 4.5%.

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“…Further, hydroxocobalamin (OH-cbl), small amounts of cyanocobalamin (CN-cbl) and traces of sulfitocobalamin have also been found in biological materials, as well as analogues such as cobamides and cobinamides, which lack vitamin B 12 activity. Since vitamin B 12 deficiency is determined routinely by radioisotope dilution assays (RIDA; Nexø et al, 1982), or recently in some laboratories by chemiluminescence techniques (Zhou et al, 1991;Kilmartin et al, 2002;Mengozzi et al, 2003), where the total amount of cobalamins in biological tissues is measured, no information can be gained about their distribution and how variation could play a part in the problem of diagnosing vitamin B 12 deficiency.…”

Section: Introductionmentioning

confidence: 99%

Analysis of corrinoids in ovine tissues

Kelly¹,

Gruner

Furlong

et al. 2006

Biomed. Chromatogr.

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Corrinoids from various ovine tissue samples (liver, blood, small intestinal fluid and faeces) were analysed using a combination of high-performance liquid chromatography (HPLC) and a radioisotope dilution assay (RIDA) to estimate the distribution of corrinoids--the cobalamins hydroxocobalamin (OH-cbl), methylcobalamin (me-cbl) and 5'-deoxyadenosylcobalamin (ado-cbl), and cobalamin analogues--in these tissues. Samples were taken from either cobalt-deficient or cobalt-replete ewes, and ruminant and pre-ruminant lambs. In liver, ado-cbl predominated, followed by analogues, OH-cbl and me-cbl. Supplementation with either cobalt (ruminant) or vitamin B12 injections (pre-ruminant) increased the amount of ado-cbl and decreased analogues. In blood, OH-cbl predominated, followed by ado-cbl, analogues and me-cbl, respectively. In small intestinal fluid, the distribution from largest to smallest percentage was analogues, ado-cbl, OH-cbl and me-cbl. In faeces, analogues constituted the greatest proportion, followed by OH-cbl, ado-cbl and me-cbl, respectively. Owing to the small sample sizes only cautionary interpretations can be made. In contrast to humans, where me-cbl constitutes the highest proportion of corrinoids in plasma and ado-cbl in the liver, in sheep the amount of ado-cbl was consistently higher than me-cbl in all tissues. This may be due to the higher metabolic need of sheep for ado-cbl due to gluconeogenesis. Analogues and OH-cbl were found in each tissue, contrary to previous postulations. The much higher amount of vitamin B12 in small intestinal fluid compared with faeces indicates that a large proportion of the vitamin is absorbed by the gastro-intestinal tract.

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Chemiluminescence determination of vitamin B12 by a flow-injection method

Cited by 37 publications

References 4 publications

Flow‐chemiluminescence: a growing modality of pharmaceutical analysis

Flow‐chemiluminescence: a growing modality of pharmaceutical analysis

Simultaneous determination of twelve water- and fat-soluble vitamins by high-performance liquid chromatography with diode array detection

Analysis of corrinoids in ovine tissues

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