Accurate determination of the heat release rate (HRR) information in ammonia flames is pivotal for practical applications of ammonia fuel in industrial combustion devices. However, the direct measurement of HRR in real-world applications is extremely challenging. In this study, we employed numerical simulation with detailed chemistry to assess the potential of using excited-state species and combinations of excited-state and ground-state species as HRR markers in laminar premixed ammonia−hydrogen−air flames. Two criteria were defined to evaluate the performance of the targeted HRR markers. The study revealed that single excited-state species generally exhibited limited performance as HRR markers, particularly in predicting both the peak location and the width of HRR profiles over a wide range of conditions. Products of two species showed improved performance; specifically, [O 2 *][NH 2 *], [NH 2 ][NO 2 *], and [NH 2 ][NH 2 *] exhibited excellent performance in predicting both the peak position and width of HRR profiles across a broad range of flame conditions. Further improvements were observed with products of three species, as [NH 2 ][O 2 *][NH 2 *] and [NH 2 ][O 2 *][OH*] performed well in marking both the peak location and the width of HRR profiles across various conditions. The integrated products of the concentration for [NH 2 ][NH 2 *], [O 2 *][NH 2 *], [NH 2 ][O 2 *][NH 2 *], and [NH 2 ][O 2 *][OH*] showed positive correlations with integrated HRR intensity under lean conditions, suggesting their potential use for indirectly inferring HRR intensity of practical combustion devices operating under lean conditions. Challenges associated with measurements of O 2 * and the rationale behind the utilization of excited-state radicals as HRR markers are also discussed.