Chemiluminescence‐Based Assay to Monitor Early Oxidative Bursts in Soybean (Glycine max) Lateral Roots
Muthusaravanan Sivaramakrishnan,
Sakshi Goel,
Nikhil Ratnaparkhi
et al.
Abstract:The reactive oxygen species (ROS) burst assay is a valuable tool for studying pattern‐triggered immunity (PTI) in plants. During PTI, the interaction between pathogen recognition receptors (PRRs) and pathogen‐associated molecular patterns (PAMPs) leads to the rapid production of ROS in the apoplastic space. The resultant ROS can be measured using a chemiluminescent approach that involves the usage of horseradish peroxidase and luminol. Although several methods and protocols are available to detect early ROS bu… Show more
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