Chemiluminescence and Chemiluminescence Resonance Energy Transfer (CRET) Aptamer Sensors Using Catalytic Hemin/G-Quadruplexes

Liu, Xiaoqing; Freeman, Ronit; Golub, Eyal; Willner, Itamar

doi:10.1021/nn202799d

Cited by 264 publications

(176 citation statements)

References 54 publications

(86 reference statements)

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“…Willner's group has exploited catalytic hemin/G-quadruplex DNAzymes for the detection of DNA, metal ions, aptamer-substrate complexes, thrombin, glucose oxidase, and ATP by using QD-CRET. [142][143][144][145] The hemin/G-quadruplex DNAzyme exhibits peroxidase-like activity that can catalyze the chemiluminescent reaction between luminol and H 2 O 2 . For DNA detection, three different colors of QDs (PL at 490, 560, and 620 nm) were functionalized with three different hairpin oligonucleotide probes that were complementary to three target oligonucleotide sequences of interest.…”

Section: Bioanalysis and Bioimaging With Quantum Dotsmentioning

confidence: 99%

Quantum Dots in Bioanalysis: A Review of Applications across Various Platforms for Fluorescence Spectroscopy and Imaging

2013

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Semiconductor quantum dots (QDs) are brightly luminescent nanoparticles that have found numerous applications in bioanalysis and bioimaging. In this review, we highlight recent developments in these areas in the context of specific methods for fluorescence spectroscopy and imaging. Following a primer on the structure, properties, and biofunctionalization of QDs, we describe select examples of how QDs have been used in combination with steady-state or time-resolved spectroscopic techniques to develop a variety of assays, bioprobes, and biosensors that function via changes in QD photoluminescence intensity, polarization, or lifetime. Some special attention is paid to the use of Förster resonance energy transfer-type methods in bioanalysis, including those based on bioluminescence and chemiluminescence. Direct chemiluminescence, electrochemiluminescence, and charge transfer quenching are similarly discussed. We further describe the combination of QDs and flow cytometry, including traditional cellular analyses and spectrally encoded barcode-based assay technologies, before turning our attention to enhanced fluorescence techniques based on photonic crystals or plasmon coupling. Finally, we survey the use of QDs across different platforms for biological fluorescence imaging, including epifluorescence, confocal, and twophoton excitation microscopy; single particle tracking and fluorescence correlation spectroscopy; super-resolution imaging; near-field scanning optical microscopy; and fluorescence lifetime imaging microscopy. In each of the above-mentioned platforms, QDs provide the brightness needed for highly sensitive detection, the photostability needed for tracking dynamic processes, or the multiplexing capacity needed to elucidate complex systems. There is a clear synergy between advances in QD materials and spectroscopy and imaging techniques, as both must be applied in concert to achieve their full potential.

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Section: Bioanalysis and Bioimaging With Quantum Dotsmentioning

confidence: 99%

Quantum Dots in Bioanalysis: A Review of Applications across Various Platforms for Fluorescence Spectroscopy and Imaging

2013

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“…Then, 250 μl of 20 mM H 2 O 2 stock solution was added quickly into the cuvette and emission spectra were recorded. Two samples were prepared; sample 1 was prepared in HEPES buffer 20 mM containing EDTA 0.1 mM, DMSO 1%, triton X-100 0.025%, KCl 150 mM, pH 8; in sample 2, KCl 150 mM was replaced with NaCl 150 mM pH [16][17][18][19].…”

Section: Measurement Of Dnazyme Activitymentioning

confidence: 99%

Interaction of hemin with quadruplex DNA

et al. 2016

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A DNA enzyme with peroxidase activity is a G-quadruplex-based DNAzyme formed by hemin and G-quadruplex DNA. Activity of peroxide DNAzymes can be influenced by the structure of quadruplex DNA. In this investigation, the interaction of hemin with T30695 G-quadruplex DNA is evaluated. Molecular dynamic simulation indicates that the binding mode of hemin to G-quadruplex DNA is end-stacking, which is consistent with absorption spectroscopy. Based on fluorescence spectroscopy, hemin ejects thiazole orange from bases of four-strand DNA. Circular dichroism spectra showed that no alteration occurs in this type of DNA structure.

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“…The design can also be modified for other oxidation reactions or biocatalytic processes that can directly or indirectly produce H 2 O 2 . Additional work done by Willner's group has shown that catalysis of the luminol-H 2 O 2 reaction by the HRP-mimicking hemin/G-quadruplex DNAzyme to produce CRET-sensitized QD emission is useful for the detection of aptamer substrates such as thrombin and ATP, as well as multiple DNA sequences (Freeman et al 2011b;Liu et al 2011). For the latter, the DNAzyme sequence was hidden within stem-loop oligonucleotides that opened in the presence of complementary target DNA to enable self-assembly of the hemin/Gquadruplex DNAzyme.…”

Section: Cret and Bret-based Bioanalysis With Quantum Dotsmentioning

confidence: 99%

Semiconductor Quantum Dots and Energy Transfer for Optical Sensing and Bioanalysis: Applications

Wu¹,

Algar²

2015

Nanobiosensors and Nanobioanalyses

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Semiconductor quantum dots (QDs) are very promising materials for optical sensing and bioanalysis. This chapter builds on Chap. 10, which reviewed the optical properties of QDs and their benefits for energy transfer, by illustrating the utility of QDs and energy transfer for optical sensing and bioanalysis. Representative examples of different in vitro assays and cellular probes from the literature are described. Energy transfer mechanisms including Förster resonance energy transfer (FRET), bioluminescence and chemiluminescence resonance energy transfer (BRET and CRET), nanosurface energy transfer (NSET), and charge transfer can be used for optical signal generation in homogeneous assays, single-particle assays, and heterogeneous assays targeting bioanalytes as diverse as nucleic acids, proteins, small molecules, ions, and the activity of enzymes such as proteases, kinases, and nucleases. The importance and versatility of QDs in optical sensing and bioanalysis has been growing steadily since their introduction and will continue to grow in the near future as QD-based assays are optimized and applied to new problems, and new capabilities are developed.

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Chemiluminescence and Chemiluminescence Resonance Energy Transfer (CRET) Aptamer Sensors Using Catalytic Hemin/G-Quadruplexes

Cited by 264 publications

References 54 publications

Quantum Dots in Bioanalysis: A Review of Applications across Various Platforms for Fluorescence Spectroscopy and Imaging

Quantum Dots in Bioanalysis: A Review of Applications across Various Platforms for Fluorescence Spectroscopy and Imaging

Interaction of hemin with quadruplex DNA

Semiconductor Quantum Dots and Energy Transfer for Optical Sensing and Bioanalysis: Applications

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