Chemiluminescence and bioluminescence history – early advertising materials

Kricka, Larry J.; Stanley, Philip E.

doi:10.1002/bio.2611

Cited by 1 publication

(1 citation statement)

References 15 publications

(14 reference statements)

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“…The fact that these two methods are mainly based on solid-phase reactions leads to a lack of sensitivity, accuracy and precision in clinical applications [ 24 , 25 ]. In contrast to these two methods, chemiluminescence is based on liquid-phase reaction which makes it can give sufficient contact area and chance between substrate and reactants and therefore has obvious advantages in linear range, sensitivity, precision, accuracy and good detection stability, and the detection is fast and convenient with fully automated instruments [ 26 ]. In this study, a sandwich chemiluminescence assay was established to test the Cys C in the human serum.…”

Section: Introductionmentioning

confidence: 99%

A magnetic solid phase chemiluminescent immunoassay for quantification of Cystatin C in human serum

He,

Liang,

Wang

et al. 2023

BMC Biotechnol

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A chemiluminescent immunoassay for human serum Cystatin C (Cys C) was established using a direct-antibody sandwich model. The immunoassay kit uses magnetic separation technology, using magnetic particles as the reaction solid phase, alkaline phosphatase as the marker enzyme, and a new chemiluminescent substrate APLS as the substrate. It has the characteristics of high sensitivity and short reaction time. This product uses high-affinity antibodies, resulting in a high specificity. The established method showed good accuracy, uniformity, and stability. The limit of detection was 2.39 ng/mL. The intra-assay coefficient of variation (CV) was 3.36%–6.00%, the interassay CV was 4.12%–5.35%, and the recovery rate was 99.07%. The correlation coefficient (r) of Cys-C kit was 0.999388 ≥ 0.9900. The accuracy of the developed method was tested by automatic chemiluminescence instrument (P > 0.05). The lowest titer was 0.92500, and the highest was 1.10000. The developed method showed a good correlation with the product from Roche by comparing these two kits in 240 clinical samples from China. In total, 1392 clinical patient from China samples were measured using the reagent kit developed in this study.

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Section: Introductionmentioning

confidence: 99%