Chemically synthesized silver nanoparticles induced physio-chemical and chloroplast ultrastructural changes in broad bean seedlings

Abdel-Aziz, Heba M. M.; Rizwan, Muhammad

doi:10.1016/j.chemosphere.2019.07.035

Cited by 37 publications

(14 citation statements)

References 27 publications

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“…The results also showed an increasing number of starch grains in the chloroplasts at the low concentration of AgNPs (20 mg/L) and decreased at higher concentrations. These results are consistent with the results of [ 64 ] in Vicia faba seedlings after seed priming by 10 ppm of AgNPs. Increasing starch grains in leaf cells may be the result of increasing photosynthetic machinery and can stimulate the accumulation of starch as a defense response against AgNPs stress [ 21 ].…”

Section: Discussionsupporting

confidence: 93%

“…Changes in chloroplast size and shape and chloroplast rupture was observed in tobacco seedlings [ 18 , 19 ]. The irregular shape of chloroplasts with protrusions and less stacking of grana was also shown in broad bean seedlings after seed priming with high concentrations of AgNPs [ 64 ]. Plastoglobuli increased in number and size in leaf cells and [ 20 ] found the same results where leaves of tobacco plants treated with AgNP-PVP and AgNP-CTAB were containing thinner and longer chloroplasts and large plastoglobules compared to the control.…”

Section: Discussionmentioning

confidence: 99%

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Ultrastructural and molecular implications of ecofriendly made silver nanoparticles treatments in pea (Pisum sativum L.)

Labeeb

Badr

Haroun

et al. 2022

Journal of Genetic Engineering and Biotechnology

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Background Silver nanoparticles (AgNPs) are the most widely used nanomaterial in agricultural and environmental applications. In this study, the impact of AgNPs solutions at 20 mg/L, 40 mg/L, 80 mg/L, and 160 mg/L on cell ultrastructure have been examined in pea (Pisum sativum L) using a transmission electron microscope (TEM). The effect of AgNPs treatments on the α, β esterase (EST), and peroxidase (POX) enzymes expression as well as gain or loss of inter-simple sequence repeats (ISSRs) markers has been described. Results Different structural malformations in the cell wall and mitochondria, as well as plasmolysis and vacuolation were recorded in root cells. Damaged chloroplast and mitochondria were frequently observed in leaves and the osmiophilic plastoglobuli were more observed as AgNPs concentration increased. Starch grains increased by the treatment with 20 mg/L AgNPs. The expressions of α, β EST, and POX were slightly changed but considerable polymorphism in ISSR profiles, using 17 different primers, were scored indicating gain or loss of gene loci as a result of AgNPs treatments. This indicates considerable variations in genomic DNA and point mutations that may be induced by AgNPs as a genotoxic nanomaterial. Conclusion AgNPs may be used to induce genetic variation at low concentrations. However, considerations should be given to the uncontrolled use of nanoparticles and calls for evaluating their impact on plant growth and potential genotoxicity are justified.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Ultrastructural and molecular implications of ecofriendly made silver nanoparticles treatments in pea (Pisum sativum L.)

Labeeb

Badr

Haroun

et al. 2022

Journal of Genetic Engineering and Biotechnology

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“…Despite the extensive research on the toxicity effects of AgNPs on plants, there are reports on induced seed germination and plant growth upon exposure to AgNPs [ 88 , 89 ]. This could be due to AgNPs’ role in improving water and nutrient uptake [ 90 ].…”

Section: Environmental and Toxicological Effects Of Agnpsmentioning

confidence: 99%

Silver Nanoparticle’s Toxicological Effects and Phytoremediation

et al. 2021

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The advancement in nanotechnology has brought numerous benefits for humans in diverse areas including industry, medicine, and agriculture. The demand in the application of nanomaterials can result in the release of these anthropogenic materials into soil and water that can potentially harm the environment by affecting water and soil properties (e.g., soil texture, pH, organic matter, and water content), plants, animals, and subsequently human health. The properties of nanoparticles including their size, surface area, and reactivity affect their fate in the environment and can potentially result in their toxicological effects in the ecosystem and on living organisms. There is extensive research on the application of nano-based materials and the consequences of their release into the environment. However, there is little information about environmentally friendly approaches for removing nanomaterials from the environment. This article provides insight into the application of silver nanoparticles (AgNPs), as one of the most commonly used nanomaterials, their toxicological effects, their impacts on plants and microorganisms, and briefly reviews the possibility of remediation of these metabolites using phytotechnology approaches. This article provides invaluable information to better understand the fate of nanomaterials in the environment and strategies in removing them from the environment.

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“…Besides PVP, polyethylene glycol (PEG) and polyvinyl alcohol (PVA) have also been frequently used for AgNP stabilization in both plant [ 52 , 53 , 54 , 55 , 56 , 57 ] and algal research [ 19 , 20 , 26 , 28 ], while GA, a natural polymer consisting of polysaccharides and glycoproteins, has mostly been utilized in plant studies [ 10 , 46 , 58 , 59 ]. Considering the electrostatic stabilization of AgNPs, citrate is the most commonly applied coating that provides a negative charge, and it has been employed in many toxicology studies performed on both plants [ 47 , 50 , 51 , 60 , 61 , 62 , 63 ] and algae [ 20 , 21 , 22 , 29 , 33 , 37 , 40 , 41 , 64 ]. On the other hand, positively charged AgNPs have been scarcely used in plant studies and were usually obtained by application of cationic surfactant CTAB [ 50 , 51 , 65 ], although didecyldimethylammonium bromide (DDAB) [ 66 ] or polyhexamethylene biguanide (PHMB) [ 67 ] have also been employed.…”

Section: Introductionmentioning

confidence: 99%

Surface Coating-Modulated Phytotoxic Responses of Silver Nanoparticles in Plants and Freshwater Green Algae

et al. 2021

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Silver nanoparticles (AgNPs) have been implemented in a wide range of commercial products, resulting in their unregulated release into aquatic as well as terrestrial systems. This raises concerns over their impending environmental effects. Once released into the environment, they are prone to various transformation processes that modify their reactivity. In order to increase AgNP stability, different stabilizing coatings are applied during their synthesis. However, coating agents determine particle size and shape and influence their solubility, reactivity, and overall stability as well as their behavior and transformations in the biological medium. In this review, we attempt to give an overview on how the employment of different stabilizing coatings can modulate AgNP-induced phytotoxicity with respect to growth, physiology, and gene and protein expression in terrestrial and aquatic plants and freshwater algae.

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Chemically synthesized silver nanoparticles induced physio-chemical and chloroplast ultrastructural changes in broad bean seedlings

Cited by 37 publications

References 27 publications

Ultrastructural and molecular implications of ecofriendly made silver nanoparticles treatments in pea (Pisum sativum L.)

Ultrastructural and molecular implications of ecofriendly made silver nanoparticles treatments in pea (Pisum sativum L.)

Silver Nanoparticle’s Toxicological Effects and Phytoremediation

Surface Coating-Modulated Phytotoxic Responses of Silver Nanoparticles in Plants and Freshwater Green Algae

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