Chemically Modified Hydrogel-Filled Nanopores: A Tunable Platform for Single-Molecule Sensing

Sulaiman, Dana Al; Cadinu, Paolo; Ivanov, Aleksandar P.; Edel, Joshua B.; Ladame, Sylvain

doi:10.1021/acs.nanolett.8b03111

Cited by 49 publications

(68 citation statements)

References 63 publications

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“…2c ), indicating that multiplexing of ≈10 targets could be feasible with the length of λ-DNA (48.5 kbp). Furthermore, the resolution can be improved via the reconstruction of nanopores 53 , 54 , using higher bandwidth 55 or smaller pore size 56 . In addition, the capability can be multiplied by encoding multi-colour fluorophores to the carriers.…”

Section: Discussionmentioning

confidence: 99%

Single-molecule amplification-free multiplexed detection of circulating microRNA cancer biomarkers from serum

et al. 2021

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MicroRNAs (miRNAs) play essential roles in post-transcriptional gene expression and are also found freely circulating in bodily fluids such as blood. Dysregulated miRNA signatures have been associated with many diseases including cancer, and miRNA profiling from liquid biopsies offers a promising strategy for cancer diagnosis, prognosis and monitoring. Here, we develop size-encoded molecular probes that can be used for simultaneous electro-optical nanopore sensing of miRNAs, allowing for ultrasensitive, sequence-specific and multiplexed detection directly in unprocessed human serum, in sample volumes as small as 0.1 μl. We show that this approach allows for femtomolar sensitivity and single-base mismatch selectivity. We demonstrate the ability to simultaneously monitor miRNAs (miR-141-3p and miR-375-3p) from prostate cancer patients with active disease and in remission. This technology can pave the way for next generation of minimally invasive diagnostic and companion diagnostic tests for cancer.

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Section: Discussionmentioning

confidence: 99%

Single-molecule amplification-free multiplexed detection of circulating microRNA cancer biomarkers from serum

et al. 2021

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“…In other words, the fast transport of the analyte molecules represents a current obstacle hindering the development of SERS-based flow-through sensors. Recently, different approaches were developed to integrate electrical, optical, and thermal forces to control plasmonic nano-object motion at the nanoscale 6–8 and to slow down molecule transport 9–11 . However, reports that combine these effects to demonstrate single-molecule SERS in the flow-through scheme are still missing.…”

Section: Introductionmentioning

confidence: 99%

SERS discrimination of single DNA bases in single oligonucleotides by electro-plasmonic trapping

Huang¹,

Mousavi²,

Zhao³

et al. 2019

Nat Commun

159

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Surface-enhanced Raman spectroscopy (SERS) sensing of DNA bases by plasmonic nanopores could pave a way to novel methods for DNA analyses and new generation single-molecule sequencing platforms. The SERS discrimination of single DNA bases depends critically on the time that a DNA strand resides within the plasmonic hot spot. In fact, DNA molecules flow through the nanopores so rapidly that the SERS signals collected are not sufficient for single-molecule analysis. Here, we report an approach to control the residence time of molecules in the hot spot by an electro-plasmonic trapping effect. By directly adsorbing molecules onto a gold nanoparticle and then trapping the single nanoparticle in a plasmonic nanohole up to several minutes, we demonstrate single-molecule SERS detection of all four DNA bases as well as discrimination of single nucleobases in a single oligonucleotide. Our method can be extended easily to label-free sensing of single-molecule amino acids and proteins.

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“…Through many experimental and theoretical studies conducted in the last decades, it has been concluded that the ICR phenomenon of nanochannels/nanopores can be attributed to their asymmetric characteristics. These include, for instance, geometry [20–28], surface charge [29, 30], chemical composition [31, 32], wettability [33], and external applied fields such as pH gradient [34], electrolyte concentration gradient [35], and pressure gradient [36]. Among these, geometry‐induced ICR draws much attention because the associated nanochannel fabrication methods are convenient and controllable.…”

Section: Introductionmentioning

confidence: 99%

Ion current rectification behavior of a nanochannel having nonuniform cross‐section

2020

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Ion current rectification behavior of a nanochannel having nonuniform cross-sectionDue to its versatile applications in biotechnology, ion current rectification (ICR), which arises from the asymmetric nature of the ion transport in a nanochannel, has drawn much attention, recently. Here, the ICR behavior of a pH-regulated nanochannel comprising two series connected cylindrical nanochannels of different radii is examined theoretically, focusing on the influences of the radii ratio, the length ratio, the bulk concentration, and the solution pH. The results of numerical simulation reveal that the rectification factor exhibits a local maximum with respect to both the radii ratio and the length ratio. The values of the radii ratio and the length ratio at which the local maximum in the rectification factor occur depend upon the level of the bulk salt concentration. The rectification factor also shows a local maximum as the solution pH varies. Among the factors examined, the solution pH influences the ICR behavior of the nanochannel most significantly.Abbreviations: AAO, anodic aluminum oxide; EDL, electric double layer; ICR, ion current rectification; IEP, isoelectric point been concluded that the ICR phenomenon of nanochannels/nanopores can be attributed to their asymmetric characteristics. These include, for instance, geometry [20][21][22][23][24][25][26][27][28], surface charge [29, 30], chemical composition [31, 32], wettability [33], and external applied fields such as pH gradient [34], electrolyte concentration gradient [35], and pressure gradient [36]. Among these, geometry-induced ICR draws much attention because the associated nanochannel fabrication methods are convenient and controllable. These include, for example, track-etching [37], focused ion beam sculpting [38], and electron-beam lithography [39]. Through these methods, various types of nanochannels have been fabricated such as cylindrical [27], conical [22], hourglass-shaped [26], cigar-shaped [20], bullet-shaped [25], dumbbell-shaped [21], and funnelshaped nanochannels [23]. The ICR behavior of an asymmetric nanochannel can be influenced by factors including ionic species [40], ion concentration [35, 41], applied electric potential [40], pH [42, 43], temperature [44], and nanochannel length and its opening radii [24,45,46]. The cone angle [24] and the ratio of cylindrical segment/conical segment [23] are important to conical and funnel-shaped nanochannels, respectively.Previous studies regarding asymmetric nanochannel focused mainly on polymeric material such as polycarbonate Color online: See article online to view Figs. 1-8 in color.

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Chemically Modified Hydrogel-Filled Nanopores: A Tunable Platform for Single-Molecule Sensing

Cited by 49 publications

References 63 publications

Single-molecule amplification-free multiplexed detection of circulating microRNA cancer biomarkers from serum

Single-molecule amplification-free multiplexed detection of circulating microRNA cancer biomarkers from serum

SERS discrimination of single DNA bases in single oligonucleotides by electro-plasmonic trapping

Ion current rectification behavior of a nanochannel having nonuniform cross‐section

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