Chemically Induced Dynamic Nuclear PolarizationUpdate based on original article by Heinz D. Roth,<i>Encyclopedia of Magnetic Resonance</i>, ©1996, John Wiley &amp; Sons Ltd.

Roth, Heinz D.

doi:10.1002/9780470034590.emrstm0077.pub2

Cited by 2 publications

(2 citation statements)

References 118 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The fruit is a structure that develops in flowering plants from the gynoecia after fertilization [ 5 ]. Different fruit types in angiosperms evolved with free (apocarpous) or (partly) fused carpels (syncarpous), a dry or fleshy texture, various pericarp layer differentiations, and different ways of opening [ 6 – 8 ]. The most abundant fruit types are dry dehiscent, dry indehiscent and fleshy, with frequent transitions between the different fruit types [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Evolution and development of fruits of Erycina pusilla and other orchid species

Pramanik,

Becker,

Roessner

et al. 2023

PLoS ONE

View full text Add to dashboard Cite

Fruits play a crucial role in seed dispersal. They open along dehiscence zones. Fruit dehiscence zone formation has been intensively studied in Arabidopsis thaliana. However, little is known about the mechanisms and genes involved in the formation of fruit dehiscence zones in species outside the Brassicaceae. The dehiscence zone of A. thaliana contains a lignified layer, while dehiscence zone tissues of the emerging orchid model Erycina pusilla include a lipid layer. Here we present an analysis of evolution and development of fruit dehiscence zones in orchids. We performed ancestral state reconstructions across the five orchid subfamilies to study the evolution of selected fruit traits and explored dehiscence zone developmental genes using RNA-seq and qPCR. We found that erect dehiscent fruits with non-lignified dehiscence zones and a short ripening period are ancestral characters in orchids. Lignified dehiscence zones in orchid fruits evolved multiple times from non-lignified zones. Furthermore, we carried out gene expression analysis of tissues from different developmental stages of E. pusilla fruits. We found that fruit dehiscence genes from the MADS-box gene family and other important regulators in E. pusilla differed in their expression pattern from their homologs in A. thaliana. This suggests that the current A. thaliana fruit dehiscence model requires adjustment for orchids. Additionally, we discovered that homologs of A. thaliana genes involved in the development of carpel, gynoecium and ovules, and genes involved in lipid biosynthesis were expressed in the fruit valves of E. pusilla, implying that these genes may play a novel role in formation of dehiscence zone tissues in orchids. Future functional analysis of developmental regulators, lipid identification and quantification can shed more light on lipid-layer based dehiscence of orchid fruits.

show abstract

Section: Introductionmentioning

confidence: 99%

Evolution and development of fruits of Erycina pusilla and other orchid species

Pramanik,

Becker,

Roessner

et al. 2023

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…= These authors contributed equally to this work. z E-mail: chemlixi@whut.edu.cn; polymers@whut.edu.cn Graphene (GR), which was first being found by Geim in 2004 with the two-dimensional planar structure, had caused great attention by the researchers because of its outstanding advantages covering the large specific surface area, high mechanical strength, good biocompatibility, as well as the thermal conductivity, et al 22,23 Moreover, relevant reports 24 had also proved that the aromatic regions of GR can offer active sites for interaction with other aromatic molecules through π-π supramolecular interactions. Therefore, GR and its derivatives have been considered as promise materials in various areas, as well as in the electrochemical sensing platform for specific DNA detection.…”

mentioning

confidence: 99%

An Electrochemical DNA Sensing Platform Using Carboxyl Functionalized Graphene as the Electrode Modified Material

Cheng

Yang

et al. 2017

J. Electrochem. Soc.

View full text Add to dashboard Cite

With the expected characteristics of both promoting electric transfer rate and immobilizing -NH 2 labeled probe for the construction of electrochemical sensing platform for DNA determination, the carboxyl functionalized graphene (CFGR-COOH) was carried out from the carboxylation of graphene oxide (GO), partly reduction, addition of cyan groups (-CN). The X-ray photoelectron spectroscopy (XPS) results indicated the successfully prepared expected materials. Three kinds of novel electrochemical DNA biosensors were developed at CFGR-COOH modified glassy carbon electrode (CFGR-COOH/GCE), targeting at identifying specific DNA, the CFGR-COOH and PANI modified platinum electrode (CFGR-COOH/PANI/Pt), aiming at the further increase for the electrochemical performance of the prior biosensor with the synergistic effect between those two materials, and the hairpin probe/ horseradish peroxidase (HRP) labeled CFGR-COOH/GCE, which was constructed to prove the promise utilization of CFGR-COOH in various sensing platforms. Through the differential pulse voltammetry (DPV) detection, the linear ranges of 1.0 × 10 −6 to 1.0 × 10 −13 mol · L −1 , 1.0 × 10 −6 to 1.0 × 10 −14 mol · L −1 and 1.0 × 10 −6 to 1.0 × 10 −10 mol · L −1 were obtained for the above biosensors, coupling with the detection limits of 4.0 × 10 −14 mol · L −1 , 5.8 × 10 −15 mol · L −1 and 7.0 × 10 −11 mol · L −1 , respectively. The as-prepared CFGR-COOH showed potential application prospect as a valuable modification material in DNA sensing platforms.With the increase of understanding the key role of nucleic acids in providing and transferring enormous genetic information in organisms, considerable efforts have been devoted to the development of sequence-specific DNA detection. 1,2 Electrochemical DNA biosensor, 3-5 a device in the position of sequence-specific DNA analysis, has been vigorously pursued because of its outstanding advantages including specificity, sensitivity, portability and low-cost compared with the conventional methods, like the polymerase chain reaction (PCR), 6 surface plasmon resonance spectroscopy 7 and some others.Generally speaking, a biorecognition system and a transverter are covered in an electrochemical DNA biosensor. The formation of a biorecognition interface is achieved by the immobilization of a specific oligonucleotide sequence on the electrode surface, while the transverter plays a role in the conversion of the hybridization events into the electrochemical signals which is realized with the contributions of electrochemical indicators or the various responses from the single stranded probe to the helix structured oligonucleotides. 8 Glassy carbon electrode, 9 platinum electrode, 10 gold electrode, 11 as well as the carbon paste electrode 12 are often utilized as the substrates for DNA electrochemical detection. Previous reports 13 have proved that the DNA hybridization events cannot completely accomplished on the bare electrode because of the weak interactions and the existed overpotentials. With the rapid development and wide utiliz...

show abstract

Chemically Induced Dynamic Nuclear PolarizationUpdate based on original article by Heinz D. Roth,Encyclopedia of Magnetic Resonance, ©1996, John Wiley & Sons Ltd.

Cited by 2 publications

References 118 publications

Evolution and development of fruits of Erycina pusilla and other orchid species

Evolution and development of fruits of Erycina pusilla and other orchid species

An Electrochemical DNA Sensing Platform Using Carboxyl Functionalized Graphene as the Electrode Modified Material

Contact Info

Product

Resources

About