Chemically defined, albumin-free human cardiomyocyte generation

Lian, Xiaojun; Bao, Xiaoping; Zilberter, Misha; Westman, Mattias; Fisahn, André; Hsiao, Cheston; Hazeltine, Laurie B.; Dunn, Kaitlin K.; Kamp, Timothy J.; Palecek, Sean P.

doi:10.1038/nmeth.3448

Cited by 132 publications

(122 citation statements)

References 9 publications

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“…This strategy also shortened the differentiation cycle compared with the embryoid body system, with [39]. TEM showed that the differentiated cardiomyocytes have a higher level of autophagy consistent with what we witnessed in the patient's myocardial tissue.…”

Section: Discussionsupporting

confidence: 80%

Generation of a Urine-Derived Ips Cell Line from a Patient with a Ventricular Septal Defect and Heart Failure and the Robust Differentiation of These Cells to Cardiomyocytes via Small Molecules

Cao

Wen

et al. 2018

Cell Physiol Biochem

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Background/Aims: Ventricular septal defects (VSDs) are one of the most common types of congenital heart malformations. Volume overload resulting from large VSDs can lead to heart failure (HF) and constitutes a major cause of pediatric HF with a series of often-fatal consequences. The etiology of VSD with HF is complex, and increasing evidence points toward a genetic basis. Indeed, we identified an L2483R mutation in the ryanodine receptor type 2 (RyR2) in a 2-month-old male patient with VSD with HF. Methods: We generated integration-free induced pluripotent stem cells from urine samples (UiPSCs) of this patient using Sendai virus containing the Yamanaka factors and characterized these cells based on alkaline phosphatase activity, pluripotency marker expression, and teratoma formation. Then, we induced the derived UiPSCs to rapidly and efficiently differentiate into functional cardiomyocytes through temporal modulation of canonical Wnt signaling with small molecules. Real-time PCR and immunofluorescence were used to verify the expression of myocardium-specific markers in the differentiated cardiomyocytes. The ultrastructure of the derived myocardial cells was further analyzed by using transmission electron microscopy. Results: The established UiPSC lines were positive for alkaline phosphatase activity, retained the RyR2 mutation, expressed pluripotency markers, and displayed differentiation potential to three germ layers in vivo. The UiPSC-derived cells showed hallmarks of cardiomyocytes, including spontaneous contraction and strong expression of cardiac-specific proteins and genes. However, compared with cardiomyocytes derived from H9 cells, they had a higher level of autophagy, implying that autophagy may play an important role in the development of VSD with HF. Conclusion: The protocol described here yields abundant myocardial cells and provides a solid platform for further investigation of the pathogenesis, pharmacotherapy, and gene therapy of VSD with HF.

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Section: Discussionsupporting

confidence: 80%

Generation of a Urine-Derived Ips Cell Line from a Patient with a Ventricular Septal Defect and Heart Failure and the Robust Differentiation of These Cells to Cardiomyocytes via Small Molecules

Cao

Wen

et al. 2018

Cell Physiol Biochem

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“…The recipient was successful on the first trial utilizing CultureTrax, a major hurdle when incorporating novel protocols to the laboratory. The recipient's results were similar to previous published studies and those ongoing in the laboratory [7,11]. In addition to cardiomyocyte differentiation, we have used CultureTrax to successfully implement the differentiation of brain microvascular endothelial cells, neurons, endothelial cells and epithelial progenitor cells all from iPSCs.…”

supporting

confidence: 81%

Software to Improve Transfer and Reproducibility of Cell Culture Methods

et al. 2018

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Cell culture is a vital component of laboratories throughout the scientific community, yet the absence of standardized protocols and documentation practice challenges laboratory efficiency and scientific reproducibility. We examined the effectiveness of a cloud-based software application, CultureTrax as a tool for standardizing and transferring a complex cell culture protocol. The software workflow and template were used to electronically format a cardiomyocyte differentiation protocol and share a digitally executable copy with a different lab user. While the protocol was unfamiliar to the recipient, they executed the experiment by solely using CultureTrax and successfully derived cardiomyocytes from human induced pluripotent stem cells. This software tool significantly reduced the time and resources required to effectively transfer and implement a novel protocol.

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“…This understanding of the major signaling pathways that govern mesoderm formation has permitted the development of robust protocols to differentiate pluripotent stem cells into cardiomyocytes (CMs) 25, 26 . These protocols rely on activation of canonical WNT signaling to induce mesoderm formation, followed by WNT inhibition to stimulate CM differentiation.…”

Section: Development Of the Cardiac Mesodermmentioning

confidence: 99%

“…These protocols rely on activation of canonical WNT signaling to induce mesoderm formation, followed by WNT inhibition to stimulate CM differentiation. These protocols are remarkably efficient, often yielding 100 CMs per input pluripotent stem cells, and at purities of over 90% 25, 26 . Efficient pluripotent stem cell to CM differentiation protocols are fundamental to using pluripotent cell differentiation systems to study cardiac differentiation 27 , model human heart disease 28 , and produce CMs as replacement therapy for heart failure 29 .…”

Section: Development Of the Cardiac Mesodermmentioning

confidence: 99%

Cardiac Regeneration

Galdos

Guo

Paige

et al. 2017

Circulation Research

126

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Palliative surgery for congenital heart disease has allowed patients with previously lethal heart malformations to survive and, in most cases, to thrive. However, these procedures often place pressure and volume loads on the heart, and over time these chronic loads can cause heart failure. Current therapeutic options for initial surgery and chronic heart failure that results from failed palliation are limited, in part, by the mammalian heart’s low inherent capacity to form new cardiomyocytes (CMs). Surmounting the heart regeneration barrier would transform the treatment of congenital, as well as acquired, heart disease, and likewise would enable development of personalized, in vitro cardiac disease models. Although these remain distant goals, studies of heart development are illuminating the path forward and suggest unique opportunities for heart regeneration, particularly in fetal and neonatal periods. Here we review major lessons from heart development that inform current and future studies directed at enhancing cardiac regeneration.

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Chemically defined, albumin-free human cardiomyocyte generation

Cited by 132 publications

References 9 publications

Generation of a Urine-Derived Ips Cell Line from a Patient with a Ventricular Septal Defect and Heart Failure and the Robust Differentiation of These Cells to Cardiomyocytes via Small Molecules

Generation of a Urine-Derived Ips Cell Line from a Patient with a Ventricular Septal Defect and Heart Failure and the Robust Differentiation of These Cells to Cardiomyocytes via Small Molecules

Software to Improve Transfer and Reproducibility of Cell Culture Methods

Cardiac Regeneration

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