Chemical variability of Rhododendron tomentosum (Ledum palustre) essential oils and their pro-apoptotic effect on lymphocytes and rheumatoid arthritis synoviocytes

Jesionek, Anna; Kokotkiewicz, Adam; Mikosik-Roczynska, Anna; Ciesielska-Figlon, Klaudia; Łuczkiewicz, Piotr; Buciński, Adam; Daca, Agnieszka; Witkowski, Jacek M.; Bryl, Ewa; Zabiegała, Bożena; Łuczkiewicz, Maria

doi:10.1016/j.fitote.2019.104402

Cited by 12 publications

(29 citation statements)

References 38 publications

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“…This species has been widely used in folk medicine against arthrosis, rheumatism, pain, wounds, fever, and cough, even if the internal use of its extracts has become rare due to it containing the toxic sesquiterpenoid ledol [ 44 ]. The antiarthritic potential of this species was recently assessed by Jesionek and coworkers [ 45 ]. The authors tested the antiproliferative activity of different EOs from R. tomentosum on CD4+ and CD8+ limphocytes, which are involved in the pathogenesis of rheumatoid arthritis.…”

Section: Essential Oils With Antiarthritic Potentialmentioning

confidence: 99%

Essential Oils and Bioactive Components against Arthritis: A Novel Perspective on Their Therapeutic Potential

Marrelli

Amodeo

Perri

et al. 2020

Plants

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Essential oils (EOs) are known to possess a number of beneficial properties. Their antimicrobial, anti-inflammatory, antioxidant, antidiabetic, and cancer-preventing activities have been extensively reported. Due to their wide use as food preservers and additives, as well as their use in agriculture, perfumes, and make-up products, these complex mixtures of volatile compounds have gained importance from a commercial point of view, not only in the pharmaceutical industry, but also in agronomic, food, cosmetic, and perfume industries. An analysis of the recent scientific literature allowed us to highlight the presence of an increasing number of studies on the potential antiarthritic properties of EOs and their main constituents, which seems to suggest a new interesting potential therapeutic application. The aim of this review is to examine the current knowledge on the beneficial effects of essential oils in the treatment of arthritic diseases, providing an overview of the reports on the in vivo and in vitro effects of EOs. Furthermore, this review critically examines the recent findings on the potential roles of the main components of EOs in the exerted beneficial effects. Obtained negative results are also reported.

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Section: Essential Oils With Antiarthritic Potentialmentioning

confidence: 99%

Essential Oils and Bioactive Components against Arthritis: A Novel Perspective on Their Therapeutic Potential

Marrelli

Amodeo

Perri

et al. 2020

Plants

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“…tomentosum EOs ( Table 2). The structure Antimicrobial in vitro, against pandemic strain Vibrio parahaemolyticus [19] Antioxidant activity in vitro, by DPPH˙ assay, ascorbic acid was used as the reference antioxidant [10] Antioxidant, using radical scavenging ABTS˙+, DPPH˙ and TROLOX equivalent assay [13] Antioxidant in vitro, by DPPH˙ assay, Fe 3+ -EDTA-H 2 O 2 deoxyribose assay, nonenzymatic lipid peroxidation of rat liver homogenate [18] Anti-proliferative and proapoptotic activity in vitro, the influence of EOs on blood lymphocytes' proliferation and apoptosis rates of synovia-derived cells was determined by flow cytometry method [20] Antithrombin [5] Antithrombin in vitro, using thrombin solution from bovine plasma [6] Hepato-protective in vivo, in rats and mice, CCl4 intoxication [21] Toxicity in vivo, tested on mice [22] Toxicity in vivo, tested on brine shrimp (Artemia sera) larvae [13] of main constituents is presented in Fig. 3.…”

Section: Main Composition Of Rhododendron Tomentosum Harmaja (Ex Ledumentioning

confidence: 99%

“…Many research works have been devoted to investigate various bioactivities, such as antibacte-rial, anticancer, anti-inflammatory, fungicidal, antioxidant, toxic, etc. properties of marsh rosemary extracts (see Table 1 for details) [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22]. The expectorant and antitussive effect of marsh rosemary tea depends on the presence of ledol in the essential oils [3].…”

Section: Introductionmentioning

confidence: 99%

Review: Marsh rosemary (Rhododendron tomentosum Harmaja (ex Ledum palustre Linn) growing in Lithuania) essential oils and their properties

Judžentienė¹

2020

chemija

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The paper reviews already (in the past fifteen years) published data from research articles on essential oils (EOs) and their biological properties (such as antioxidant, antifungal, anti-inflammatory and toxic activity) of marsh rosemary (Rhododendron tomentosum (Stokes) Harmaja (ex Ledum palustre Linnaeus, Ericaceae Juss.) growing wild in Lithuania. Rh. tomentosum is a perennial woody shrub (up to 1 m in height) with evergreen leaves and small white or white-pink sticky flowers grouped in racemes. The plant emits a strong specific smell that affects the central nervous system, and may cause nausea, headache or aggressive behaviour to some people. Only one species of the plant (ex L. palustre var. palustre) grows in Lithuania, mainly forming colonies in limited areas over all the territory. Marsh rosemary are widely used in folk medicine and homeopathy for treatment of various sickness, externally and internally as well. Most applications of Rh. tomentosum and pharmacological properties have been validated by scientific researches. In Lithuania, the plant is used for healing rheumatism, different pains, insect bites, eczema and other skin problems, infections, bronchitis, asthma, cold, tuberculosis, to block bleeding, etc. Rhododendron tomentosum H. (ex Ledum palustre L.) plants, the essential oils (EOs) of which are already investigated, were collected mostly in the Eastern part of the country (Rokiškis, Utena, Vilnius and Šalčininkai districts). Monoterpene hydrocarbons, p-cymene, myrcene and limonene, bicyclic monoterpenoid ascaridole and oxygenated sesquiterpenes, ledol, palustrol and cyclocolorenone isomers, were found to be principal compounds in the investigated EOs. Some oils contained appreciable quantities of heterocyclic compound lepalol. Most of the oils could be attributed to the ledol+palustrol or ledol+palustrol+ascaridole chemotype. Antifungal activity of Rh. tomentosum EOs was evaluated by several different techniques: against Penicillium cyclopium Westling, Trichoderma harzianum Rifai and Candida parapsilosis using an agar diffusion method; and amperometricaly, using Saccharomyces cerevisiae yeast-modified electrodes. Anti-inflammatory activity of Lithuanian marsh rosemary EOs has been revealed by subcutaneous carrageenan injection-induced hind paw oedema tests. However, antioxidant activity (tests using ABTS˙+, DPPH˙ and TROLOX) and toxic properties (against brine shrimp (Artemia sera) larvae) of Rh. tomentosum EOs were summarized.

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“…The chemical compositions of the essential oils (EOs) of the entire plant or separated aerial parts (mostly shoots) of Rh. tomentosum have been studied in some European countries [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19], the Far East and Siberia [20][21][22][23][24][25], and Asian countries [17,[26][27][28][29][30][31][32][33][34][35][36][37]. A lot research works have been focused on the evaluation of bioactivities of marsh rosemary extracts.…”

Section: Introductionmentioning

confidence: 99%

“…It was shown that Rh. tomentosum extracts, essential oils, or emitted volatiles influence herbivores' behavior [9,10,[38][39][40] and the growth of neighboring plants [24], have an impact on polypropylene plastic degradation [31], and possess analgesic [41], antibacterial [42], anticancer [43,44], antidiabetic [45,46], antifungal [47,48], anti-inflammatory [41,42,[49][50][51], antioxidant [33,46], antimicrobial [33,52], anti-proliferative, and pro-apoptotic [19], antithrombin [43], hepatoprotective [53], toxic [54], and radioprotective [55,56] properties (see Supplementary materials: Table S1 for details).…”

Section: Introductionmentioning

confidence: 99%

Toxic, Radical Scavenging, and Antifungal Activity of Rhododendron tomentosum H. Essential Oils

et al. 2020

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The chemical composition of eight (seven shoot and one inflorescence) essential oils (EOs) of Rh. tomentosum H. plants growing in Eastern Lithuania is reported. The plant material was collected during different phases of vegetation (from April to October). The oils were obtained by hydrodistillation from air-dried aerial parts (leaves and inflorescences). In total, up to 70 compounds were identified by GC−MS and GC (flame-ionization detector, FID); they comprised 91.0 ± 4.7%–96.2 ± 3.1% of the oil content. Sesquiterpene hydrocarbons (54.1 ± 1.5%–76.1 ± 4.5%) were found to be the main fraction. The major compounds were palustrol (24.6 ± 2.6%–33.5 ± 4.4%) and ledol (18.0 ± 2.9%–29.0 ± 5.0%). Ascaridol isomers (7.0 ± 2.4%–14.0 ± 2.4% in three oils), myrcene (7.2 ± 0.3% and 10.1 ± 1.3%), lepalol (3.3 ± 0.3% and 7.9 ± 3.0%), and cyclocolorenone isomers (4.1 ± 2.5%) were determined as the third main constituents. The toxic activity of marsh rosemary inflorescence and shoot oils samples was evaluated using a brine shrimp (Artemia sp.) bioassay. LC50 average values (11.23–20.50 µg/mL) obtained after 24 h of exposure revealed that the oils were notably toxic. The oil obtained from shoots gathered in September during the seed-ripening stage and containing appreciable amounts of palustrol (26.0 ± 2.5%), ledol (21.5 ± 4.0%), and ascaridol (7.0 ± 2.4%) showed the highest toxic activity. Radical scavenging activity of Rh. tomentosum EOs depended on the plant vegetation stage. The highest activities were obtained for EOs isolated from young shoots collected in June (48.19 ± 0.1 and 19.89 ± 0.3 mmol/L TROLOX (6-hydroxy-2,5,7,8-tetra-methylchromane-2-carboxylic acid) equivalent obtained by, respectively, ABTS•+ (2,2′-amino-bis(ethylbenzothiazoline-6-sulfonic acid) diammonium salt) and DPPH•(2,2-diphenyl-1-picrylhydrazyl) assays). Agar disc diffusion assay against pathogenic yeast Candida parapsilosis revealed the potential antifungal activity of EOs. An alternative investigation of antifungal activity employed mediated amperometry at yeast Saccharomyces cerevisiae-modified electrodes. The subjection of yeast cells to vapors of EO resulted in a three to four-fold increase of electrode responses due to the disruption of yeast cell membranes.

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Chemical variability of Rhododendron tomentosum (Ledum palustre) essential oils and their pro-apoptotic effect on lymphocytes and rheumatoid arthritis synoviocytes

Cited by 12 publications

References 38 publications

Essential Oils and Bioactive Components against Arthritis: A Novel Perspective on Their Therapeutic Potential

Essential Oils and Bioactive Components against Arthritis: A Novel Perspective on Their Therapeutic Potential

Review: Marsh rosemary (Rhododendron tomentosum Harmaja (ex Ledum palustre Linn) growing in Lithuania) essential oils and their properties

Toxic, Radical Scavenging, and Antifungal Activity of Rhododendron tomentosum H. Essential Oils

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