Chemical transformations of succinic acid recovered from fermentation broths by a novel direct vacuum distillation-crystallisation method

Luque, Rafael; Lin, Carol Sze Ki; Du, Chenyu; Macquarrie, Duncan J.; Koutinas, Apostolis; Wang, Ruohang; Webb, Colin; Clark, James H.

doi:10.1039/b813409j

Cited by 92 publications

(96 citation statements)

References 23 publications

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“…The broth was purified by the addition of 12.5 w/v% activated carbon (Sigma Aldrich, Darco 100 mesh) with stirring at 350 rpm for 1 h. 33 Activated carbon was removed via vacuum filtration (0.2-µm PES filter assembly, Nalgene).…”

Section: Purification and Crystallizationmentioning

confidence: 99%

Adipic acid production from lignin

Vardon

Franden

Johnson

et al. 2015

Energy Environ. Sci.

529

518

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Lignin is an alkyl-aromatic polymer present in plant cell walls for defense, structure, and water transport. Despite exhibiting a high-energy content, lignin is typically slated for combustion in modern biorefineries due to its inherent heterogeneity and recalcitrance, whereas cellulose and hemicellulose are converted to renewable fuels and chemicals. However, it is critical for the viability of third-generation biorefineries to valorize lignin alongside polysaccharides. To that end, we employ metabolic engineering, separations, and catalysis to convert lignin-derived species into cis,cis-muconic acid, for subsequent hydrogenation to adipic acid, the latter being the most widely produced dicarboxylic acid. First, Pseudomonas putida KT2440 was metabolically engineered to funnel lignin-derived aromatics to cis,cis-muconate, which is an atom-efficient biochemical transformation. This engineered strain was employed in fed-batch biological cultivation to demonstrate a cis,cis-muconate titer of 13.5 g/L in 78.5 h from a model lignin-derived compound. cis,cis-Muconic acid was recovered in high purity (>97%) and yield (74%) by activated carbon treatment and crystallization (5°C, pH 2). Pd/C was identified as a highly active catalyst for cis,cis-muconic acid hydrogenation to adipic acid with high conversion (>97%) and selectivity (>97%). Under surface reaction controlling conditions (24°C, 24 bar, ethanol solvent), purified cis,cis-muconic acid exhibits a turnover frequency of 23-30 sec -1 over Pd/C, with an apparent activation energy of 70 kJ/mol. Lastly, cis,cis-muconate was produced with engineered P. putida grown on a biomass-derived, lignin-enriched stream, demonstrating an integrated strategy towards lignin valorization to an important commodity chemical.

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Section: Purification and Crystallizationmentioning

confidence: 99%

Adipic acid production from lignin

Vardon

Franden

Johnson

et al. 2015

Energy Environ. Sci.

529

518

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“…paid to the microbial conversion (Luque et al, 2009). In most of succinic acid fermentation processes, the production was not efficient.…”

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confidence: 99%

Kinetic evaluation of products inhibition to succinic acid producers Escherichia coli NZN111, AFP111, BL21, and Actinobacillus succinogenes 130ZT

Wang

et al. 2010

J Microbiol.

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Succinic acid is one of the platform compounds and its production via natural feedstocks has drawn worldwide concerns. To evaluate the inhibitory effects of fermentation products on the growth of Actinobacillus succinogenes 130Z(T) and Escherichia coli NZN111, AFP111, BL21, fermentations with addition of individual products in medium were carried out. The cell growth was inhibited when the concentrations of formate, acetate, lactate, and succinate were at range of 8.8-17.6 g/L, 10-40 g/L, 9-18 g/L, and 10-80 g/L, respectively. For these two species of bacteria, E. coli was more resistant to acid products than A. succinogenes, while both endured succinate rather than by-products. As a result of end product inhibition, succinate production yield by A. succinogenes decreased from 1.11 to 0.49 g/g glucose. Logistic and Monod mathematical models were presented to simulate the inhibition kinetics. The Logistic model was found more suitable for describing the overall synergistic inhibitory effects.

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“…A bio-based varnish made from succinic acid, Bio-SA TM , will also be tested. This varnish is obtained by catalysation of biosourced succinic acid made by fermentation of fructose corn syrup [8]. Thus it is a more eco-friendly varnish made using renewable feedstock [8].…”

Section: Introductionmentioning

confidence: 99%

Surface Resistance of Jute Fibre/Polylactic Acid Biocomposite to Wet Heat

Zandvliet

Bandyopadhyay

Ray

2015

J. Inst. Eng. India Ser. D

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Jute fibre/polylactic acid (PLA) composite is of special interest because both resin and reinforcement come from renewable resources. Thus, it could be a more ecofriendly alternative to glass fibre composite [1] and to conventional wood-based panels made with phenolformaldehyde resin which present many drawbacks for the workers and the environment [2]. Yet the water affinity of the natural fibres, the susceptibility of PLA towards hydrolysis and the low glass transition of the PLA raise a question about the surface resistance of such composites to wet heat in service condition for a furniture application [3]. In this work, the surface resistance of PLA/jute composite alone and with two different varnishes are investigated in regard to an interior application following the standard test method in accordance to BS EN 18721:2009: ''Furniture: assessment of surface resistance to wet heat''. It is compared to two common wood based panels, plywood and hardboard. After test, the composite material surface is found to be more affected than plywood and hardboard, but it becomes resistant to wet heat when a layer of biosourced varnish or petrol-based polyurethane varnish are applied on the surface.

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Chemical transformations of succinic acid recovered from fermentation broths by a novel direct vacuum distillation-crystallisation method

Cited by 92 publications

References 23 publications

Adipic acid production from lignin

Adipic acid production from lignin

Kinetic evaluation of products inhibition to succinic acid producers Escherichia coli NZN111, AFP111, BL21, and Actinobacillus succinogenes 130ZT

Surface Resistance of Jute Fibre/Polylactic Acid Biocomposite to Wet Heat

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