Chemical synthesis and <i>in vitro</i> biocompatibility tests of poly (<scp>L</scp>‐lactic acid)

Jahno, Vanusca Dalosto; Ribeiro, Gabriela Benderóvicz Mendes; Santos, Luís Alberto dos; Ligabue, Rosane Angélica; Einloft, Sandra; Ferreira, Maidy Rehder Wimmers; Bombonato-Prado, Karina Fittipaldi

doi:10.1002/jbm.a.31210

Cited by 33 publications

(19 citation statements)

References 22 publications

(21 reference statements)

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“…This can be attributed to degradation of the amorphous regions of the polymer by hydrolysis; the structures showed mobility at lower temperatures because of chain scission [28,29], the most obvious shift after 28 days of exposure of the polymer to the animal organism. The absorption and hydrolysis processes when tested in vivo favored the degradation of the PLA [30,31].…”

Section: Resultsmentioning

confidence: 99%

Biocompatibility Assessment of Poly(lactic acid) Films after Sterilization with Ethylene Oxide in Histological Study In Vivo with Wistar Rats and Cellular Adhesion of Fibroblasts In Vitro

Savaris

Braga

Santos

et al. 2017

International Journal of Polymer Science

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Biomaterials must meet certain fundamental requirements for their usage in living beings, such as biocompatibility, bifunctionality, and sterilizability, without having chemical and structural changes. The biocompatibility of poly(lactic acid) (PLA) films, shaped by compression, was evaluated after sterilization by ethylene oxide by a histological in vivo test with Wistar rats and cytotoxicity in cell adhesion in vitro. The cytotoxicity test was performed by the reduction of tetrazolium salt (MTT). Thermal and chemical changes in PLA films concerning the proposed sterilization process and characteristics were not observed to evidence polymer degradation due to sterilization. The analysis of the cytotoxicity by the MTT method has shown that the sterilized PLA films are not cytotoxic. The adhesion and proliferation of fibroblasts on PLA films were homogeneously distributed over the evaluation period, showing an elongated appearance with unnumbered cytoplasmic extensions and cell-cell interactions. By examining the biocompatibility in a histological study, a mild tissue inflammation was observed with the presence of fibrosis in the samples that had been exposed for 21 days in the rats' bodies. PLA films sterilized with ethylene oxide did not exhibit cell adhesion in vitro and toxicity to the surrounding tissue in vivo and they may be used in future in vivo testing, according to histological findings in Wistar rats in the present study.

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Section: Resultsmentioning

confidence: 99%

Biocompatibility Assessment of Poly(lactic acid) Films after Sterilization with Ethylene Oxide in Histological Study In Vivo with Wistar Rats and Cellular Adhesion of Fibroblasts In Vitro

Savaris

Braga

Santos

et al. 2017

International Journal of Polymer Science

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“…Then bone specimens were fixed and embedded following the same protocol as the intramuscular specimens. Histological slices were obtained by a microcutting and grinding techniques adapted from Donath . One central longitudinal slice per specimen was prepared with a diamond wheel saw (Well 3241, ESCIL, Chassieu, France, diameter 220 μm, grain size: 40 μm, length 10 mm).…”

Section: Methodsmentioning

confidence: 99%

In vitro and in vivo evaluation of a polylactic acid‐bioactive glass composite for bone fixation devices

et al. 2015

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Poly(lactic acid) is nowadays among the most used bioabsorbable materials for medical devices. To promote bone growth on the material surface and increase the degradation rate of the polymer, research is currently focused on organic-inorganic composites by adding a bioactive mineral to the polymer matrix. The purpose of this study was to investigate the ability of a poly(L,DL-lactide)-Bioglass® (P(L,DL)LA-Bioglass(®) 45S5) composite to be used as a bone fixation device. In vitro cell viability testing of P(l,dl)LA based composites containing different amounts of Bioglass(®) 45S5 particles was investigated. According to the degradation rate of the P(L,DL)LA matrix and the cytocompatibility experiments, the composite with 30 wt % of Bioglass® particles seemed to be the best candidate for further investigation. To study its behavior after immersion in simulated physiological conditions, the degradation of the composite was analyzed by measuring its weight loss and mechanical properties and by proceeding with X-ray tomography. We demonstrated that the presence of the bioactive glass significantly accelerated the in vitro degradation of the polymer. A preliminary in vivo investigation on rabbits shows that the addition of 30 wt % of Bioglass(®) in the P(L,DL)LA matrix seems to trigger bone osseointegration especially during the first month of implantation. This composite has thus strong potential interest for health applications.

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“…Among the variety of biomaterials used in the biomedical field, poly (L-lactic acid) (PLLA) is one of the polymers most widely employed for the regeneration of different tissues or organs, like bone [4,5], cartilage [6] and skin [7], because of its relatively good mechanical and manufacturing properties. However, the biomedical applications of PLLA are hampered to a certain extent by its high hydro -phobicity and lack of physiological activity [8,9]. It has been shown in a number of studies that PLLA does not provide a favorable surface for cell attachment and proliferation due to lack of specific cellrecognition signals [10].…”

Section: Introductionmentioning

confidence: 99%

LBL coating of type I collagen and hyaluronic acid on aminolyzed PLLA to enhance the cell-material interaction

Zhao¹,

Zhou²,

Li³

et al. 2014

Express Polym. Lett.

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Abstract. The aim of the present work is to assemble extracellular matrix components onto poly (L-lactic acid) (PLLA) films using layer-by-layer (LBL) depositing method to enhance the cell-material interaction. To introduce charges onto the hydrophobic and neutral PLLA surface so that the electronic assembly can be processed, poly (ethylene imine) (PEI) was covalently bonded to modify the PLLA films. Positively charged collagen I (Col I) was then deposited onto the aminolyzed PLLA film surface in a LBL assembly manner using hyaluronic acid (HA) as a negatively charged polyelectrolyte. The PEI modification efficiency was monitored via X-ray photoelectron spectroscopy (XPS) measurements. The results of Surface Plasmon Resonance (SPR) and Water contact angle (WCA) monitoring the LBL assemble process presented that the HA/Col I deposited alternately onto the PLLA surface. The surface topography of the films was observed by Atomic force microscope (AFM). In vitro osteoblast culture found that the presence of Col I layer greatly improved the cytocompatibility of the PLLA films in terms of cell viability, cell proliferation and Alkaline Phosphatase (ALP) expression. Furthermore, osteoblast extensions were found to be directed by contact guidance of the aligned Col I fibrils. Thus, these very flexible systems may allow broad applications for improve the bioactivity of polymeric materials, which might be a potential application for bone tissue engineering.

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Chemical synthesis and in vitro biocompatibility tests of poly (L‐lactic acid)

Cited by 33 publications

References 22 publications

Biocompatibility Assessment of Poly(lactic acid) Films after Sterilization with Ethylene Oxide in Histological Study In Vivo with Wistar Rats and Cellular Adhesion of Fibroblasts In Vitro

Biocompatibility Assessment of Poly(lactic acid) Films after Sterilization with Ethylene Oxide in Histological Study In Vivo with Wistar Rats and Cellular Adhesion of Fibroblasts In Vitro

In vitro and in vivo evaluation of a polylactic acid‐bioactive glass composite for bone fixation devices

LBL coating of type I collagen and hyaluronic acid on aminolyzed PLLA to enhance the cell-material interaction

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