Chemical synthesis and expression of the human calcitonin gene

Glycation and Post-translational Processing of Human Interferon-γ Expressed in Escherichia coli

“…Purification of rhIFN-␥-A gene coding for cysteineless hIFN-␥ was expressed constitutively (35) in E. coli XL1-Blue (supE44 hsdR17 recA1 endA1 gyrA46 thi relA1 lac Ϫ FЈ (proAB ϩ lacI q lacZ⌬M15 Tn10 (tet r ))). Transformed cells were cultured overnight at 37°C in 1-liter flasks containing 250 ml of LB medium supplemented with 10 g/ml tetracycline and 0.1% glucose.…”

Effect of Arginine on Glycation and Stability of Recombinant Human Interferon-Gamma

“…A gene coding for cysteineless hIFN-γ was expressed constitutively (10) in E. coli LE 392. Transformed cells were cultured overnight at 37 °C in 1-liter flasks containing 250 ml of LB medium supplemented with 10 µg/ml tetracycline and 0.1% glucose.…”

Role of the C-terminal chain in human interferon? stability: An electrostatic study

“…15 The two oligonucleotides necessary for modification of the hIFN␥ gene were synthesized on a Millipore Cyclon Plus DNA synthesizer (MilliGene, Division of Millipore, Burlington, MA, USA) DNA assembler and purified by electrophoresis on a polyacrylamide-urea gel. Their nucleotide sequences are as follows:…”