“…Purification of rhIFN-␥-A gene coding for cysteineless hIFN-␥ was expressed constitutively (35) in E. coli XL1-Blue (supE44 hsdR17 recA1 endA1 gyrA46 thi relA1 lac Ϫ FЈ (proAB ϩ lacI q lacZ⌬M15 Tn10 (tet r ))). Transformed cells were cultured overnight at 37°C in 1-liter flasks containing 250 ml of LB medium supplemented with 10 g/ml tetracycline and 0.1% glucose.…”
Until recently, nonenzymatic glycosylation (glycation) was thought to affect the proteins of long living eukaryotes only. However, in a recent study (Mironova, R., Niwa, T., Hayashi, H., Dimitrova, R., and Ivanov, I.
“…Purification of rhIFN-␥-A gene coding for cysteineless hIFN-␥ was expressed constitutively (35) in E. coli XL1-Blue (supE44 hsdR17 recA1 endA1 gyrA46 thi relA1 lac Ϫ FЈ (proAB ϩ lacI q lacZ⌬M15 Tn10 (tet r ))). Transformed cells were cultured overnight at 37°C in 1-liter flasks containing 250 ml of LB medium supplemented with 10 g/ml tetracycline and 0.1% glucose.…”
Until recently, nonenzymatic glycosylation (glycation) was thought to affect the proteins of long living eukaryotes only. However, in a recent study (Mironova, R., Niwa, T., Hayashi, H., Dimitrova, R., and Ivanov, I.
“…A gene coding for cysteineless hIFN-γ was expressed constitutively (10) in E. coli LE 392. Transformed cells were cultured overnight at 37 °C in 1-liter flasks containing 250 ml of LB medium supplemented with 10 µg/ml tetracycline and 0.1% glucose.…”
“…15 The two oligonucleotides necessary for modification of the hIFN␥ gene were synthesized on a Millipore Cyclon Plus DNA synthesizer (MilliGene, Division of Millipore, Burlington, MA, USA) DNA assembler and purified by electrophoresis on a polyacrylamide-urea gel. Their nucleotide sequences are as follows:…”
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