Chemical Structure of Tubular and Glomerular Basement Membranes of Human Kidney

Mahieu, P.; Winand, René

doi:10.1111/j.1432-1033.1970.tb00867.x

Cited by 77 publications

(24 citation statements)

References 33 publications

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“…The amino acid composition of GBM isolated from control rats closely resembles that of normal human (14,31,32) and bovine (33) GBM (Table I) and is similar to that found in prior studies of rat GBM (34,35). Analysis of the carbohydrate content of rat GBM revealed the presence of glucose, galactose, mannose, fucose, and glucosamine (Table II), all of which have previously been found in human and bovine GBM.…”

Section: Resultssupporting

confidence: 77%

Glomerular basement membrane: biosynthesis and chemical composition in the streptozotocin diabetic rat.

Beisswenger¹

1976

J. Clin. Invest.

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A B S T R A C T To study the effect of streptozotocin induced diabetes on glomerular basement membrane (GBM) synthesis, an isolated rat glomerular preparation has been developed, and its metabolic properties have been defined. The chemical composition of normal rat GBM isolated from this preparation closely resembles human GBM. Incubation with [U-'4C1 lysine leads to prompt incorporation of label into GBM and the subsequent appearance of labeled hydroxylysine. A 1-h lag before detection of labeled hydroxylysine in GBM suggests a delay in the release of GBM precursors. Significantly lower counts appeared in the nondialyzable fraction of the medium than in insoluble GBM during pulse-chase experiments, and labeled hydroxylysine accounted for a lower portion of the total counts in the medium (0.85%) than in the GBM (1.98%).Isolated glomeruli were prepared from streptozotocin diabetic rats of 4-6 wks duration. After incubation with [U-'4C] The chemical composition and morphology of the GBM was then studied in rats with long term streptozotocin diabetes (14 mo) to determine if the increase in hydroxylysine and glucosyl galactose and the decrease in lysine previously noted in the GBM in long-term human diabetes occurs in the rat. Except for minor changes in proline and arginine content, the chemical composition of diabetic rat GBM did not differ from age matched controls. while light microscopic studies of diabetic and control kidneys revealed equal degrees of mesangial and peripheral GBM changes. INTRODUCTIONPathologic changes in the capillary wall characterized by thickening of the basement membrane have been recognize(l in multiple vascular beds of long-term diabetics (1, 2). Diabetic intercapillary glomerulosclerosis and restultant renal failure, however, continue to be the greatest single cause of mortality and morbidity in patients with the juvenile onset type of diabetes (3,4 In addition to the basement membrane thickening observed in patients with diabetes mellitus, a distinct chemical change has been described in the GBM of some patients with diabetes of long duration (14,15). The diabetic GBM shows an increase in hydroxylysine with a concomitant decrease in lysine residues as well as an increase in the disaccharide glucosyl-galactose covalently bound to hydroxylysine. In addition, significant increases in GBM hydroxyproline and glycine content and decreases in valine and tyrosine are also observed in diabetic GBM. Since a subunit rich in glucosyl-galactose, hydroxylysine, glycine, and hydroxyproline and poor in lysine, valine, and tyrosine has been described in bovine GBM (16), it is possible that the change in diabetic GBM could represent accumulation of a similar carbohydrate rich subunit. No information on the chemical composition of GBM in experimental animals with longterm diabetes is currently available.Our current information regarding the control of GBM biosynthesis is inadequate. Several studies which utilized experimental animals have suggested that the thickening of basement membrane in diabetes ...

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Section: Resultssupporting

confidence: 77%

Glomerular basement membrane: biosynthesis and chemical composition in the streptozotocin diabetic rat.

Beisswenger¹

1976

J. Clin. Invest.

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“…GBM were isolated from normal human kidneys obtained from autopsies, according to the method of Krakower and Greenspon (11). The morphological and biochemical criteria of purity were previously described (12). The GBM were solubilized by autoclaving a suspenson of membranes at 1100C for 3 h, in Tris-acetate buffer 0.1 M, pH 7.4 (9).…”

Section: The Journal Of Clinical Investigation Volume 54 July 1974-12mentioning

confidence: 99%

“…Neutral sugars, hexosamines, and sialic acid were measured by previously described techniques (12,14); amino-acid composition was determined in a Beckman model Unichrom amino-acid analyzer (Beckman Instruments, Inc., Fullerton, Calif.) (11). The protein content was measured by the method of Lowry, Rosebrough, Farr, and Randall, with BSA as a standard (15) and the glycopeptide concentration by the ninhydrin reaction with leucine as a standard (16 (9).…”

Section: The Journal Of Clinical Investigation Volume 54 July 1974-12mentioning

confidence: 99%

“…The protein content was measured by the method of Lowry, Rosebrough, Farr, and Randall, with BSA as a standard (15) and the glycopeptide concentration by the ninhydrin reaction with leucine as a standard (16 (9). The carbohydrate and aminoacid composition of the proteins eluted with acidic buffer (9) was determined as previously described (12,14). The molecular weight of labeled and unlabeled antigens eluted with the same buffer was evaluated by acrylamide disc gel electrophoresis in dodecylsulfate (9).…”

Section: The Journal Of Clinical Investigation Volume 54 July 1974-12mentioning

confidence: 99%

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Detection of Anti-Glomercular Basement Membrane Antibodies by a Radioimmunological Technique CLINICAL APPLICATION IN HUMAN NEPHROPATHIES

Mahieu¹,

Lambert²,

Miescher³

1974

J. Clin. Invest.

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A n S T R A C T A radioimmunoassay for detection of anti-glomerular basement membrane (GBM) antibody was set up with a 70,000 mol wt GBM antigen, labeled with Iodine-'I and containing both types of oligosaccharidic chains present in the whole membrane.Separation of free radioactive antigens from antigens bound to immunoglobulins was obtained by precipitation with polyethylene glycol (mol wt, 6,000), at a final concentration of 20%. In the presence of normal human or rabbit sera, less than 20% of labeled antigens were precipitated. In the presence of rabbit anti-human GBM antibodies, up to 82% of GBM antigens were precipitated, while in the presence of sera or of kidney eluate from a patient with Goodpasture's syndrome, the precipitation of GBM antigens reached 43%. The avidity of rabbit anti-GBM antibodies for human GBM antigens is higher than that of human anti-GBM antibodies. In the case of Goodpasture's syndrome, the binding of anti-GBM antibodies to labeled antigens was inhibited more efficiently by the disaccharide-containing glycopeptide than by the heteropolysaccharide-containing glycopeptide purified from whole GBM.

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“…Their alteration in diseases such as diabetes mellitus may therefore has an influence on tissue metabolism. Although GBM has been studied by several workers (see Spiro 1972) and TBM by Machieu and Winand (1970), the membrane preparations so far isolated were possibly contaminated with basement membrane-like materials from the mesangium or collagen. Therefore, it is necessary to isolate the membranes in a high degree of purity to study the more precise chemical nature of the membranes.…”

mentioning

confidence: 99%

Chemical Compositions of Glomerular and Tubular Basement Membranes of Human Kidney

Sato

Munakata

Yoshinaga

et al. 1975

Tohoku J. Exp. Med.

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Chemical Structure of Tubular and Glomerular Basement Membranes of Human Kidney

Cited by 77 publications

References 33 publications

Glomerular basement membrane: biosynthesis and chemical composition in the streptozotocin diabetic rat.

Glomerular basement membrane: biosynthesis and chemical composition in the streptozotocin diabetic rat.

Detection of Anti-Glomercular Basement Membrane Antibodies by a Radioimmunological Technique CLINICAL APPLICATION IN HUMAN NEPHROPATHIES

Chemical Compositions of Glomerular and Tubular Basement Membranes of Human Kidney

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