Chemical structure of a polysaccharide isolated from the cell wall of Arachniotus verruculosus and A. ruber

Ahrazem, Oussama; Leal, J.A.; Prieto, Alicia; Jiménez‐Barbero, Jesús; Bernabé, Manuel

doi:10.1016/s0008-6215(01)00273-7

Cited by 14 publications

(7 citation statements)

References 25 publications

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“…The residue F, on the other hand, without any 13 C shifts induced by glycosylation, was deduced to β-T-Galf. Besides, both of the residues possessed all typical chemical shifts in comparison of the observed values with those reported in the literatures (Ahrazem, Leal, Prieto, Jiménez-Barbero, & Bernabé, 2001;Bernabé, et al, 2011;Giménez-Abián, Bernabé, Leal, Jiménez-Barbero, & Prieto, 2007;J. Leal, Jiménez-Barbero, Bernabé, & Prieto, 2008).…”

Section: Nmr Spectroscopy Analysis Of the Galactomannansupporting

confidence: 82%

Structural characterization of an α-1, 6-linked galactomannan from natural Cordyceps sinensis

et al. 2018

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An α-1, 6-linked galactomannan was isolated and purified from natural Cordyceps sinensis. The fine structure analysis of this polysaccharide was elucidated based on partial acid hydrolysis, monosaccharide composition, methylation and 1D/2D nuclear magnetic resonance (NMR) spectroscopy. Monosaccharide composition analysis revealed that this polysaccharide was mainly composed of galactose (68.65%), glucose (6.65%) and mannose (24.02%). However, after partial acid hydrolysis the percentages of galactose, glucose and mannose were changed to 3.96%, 13.82% and 82.22%, respectively. The molecular weight of this polysaccharide was 7207. Methylation and NMR analysis revealed that this galactomannan had a highly branched structure, mainly consisted of a mannan skeleton and galactofuranosyl chains. The structure of galactofuranosyl part was formed by alternating (1 5)-lined β-Galf and (1 6)-liked β-Galf or a single (1 6)-liked β-Galf, attaching to the O-2 and O-4 of the mannose chain, and terminated at β-T-Galf. The mannan core was revealed by analyzing the partial acid hydrolysate of the galactomannan and the structure was composed of (1 6)-linked α-Manp backbone, with substituted at C-2 by short chains of 2substituted Manp or Galf branches.

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Section: Nmr Spectroscopy Analysis Of the Galactomannansupporting

confidence: 82%

Structural characterization of an α-1, 6-linked galactomannan from natural Cordyceps sinensis

et al. 2018

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“…There is, again, a good correlation between the polysaccharide structure and the morphological characters in Eurotiales . It is noteworthy that the polysaccharides from Arachniotus and Gymnascella possess the above characteristics (Ahrazem et al , 2001a), which therefore supports the transfer of these genera from Onygenales to Eurotiales (Currah, 1994; Ahrazem et al , 2002b). The polysaccharides from species of Penicillium (Leal et al , 1984, 1997) and Aspergillus (Leal et al , 1995; Gómez‐Miranda et al , 2004) are similar to the polysaccharides of their teleomorphs.…”

Section: Taxonomic Valuementioning

confidence: 55%

“…It had been noted that species of Geosmithia (structures 8, 9, and 14) were related according to their polysaccharides to the genera Eupenicillium (structure 8) or Talaromyces (structure 14) . Nevertheless, three isolates of Geosmithia putterillii (structure 9) have polysaccharides similar to that isolated from Arachniotus and Gymnascella (Ahrazem et al, 2001a(Ahrazem et al, , 2002b. These results partially agree with subsequent phylogenetic analyses of rDNA sequence data, which place species of Geosmithia in Eupenicillium or Talaromyces (Ogawa et al, 1997), although the hypocrealean origin for G. putterillii is not supported by the polysaccharide structures.…”

Section: Molecular Sequencesmentioning

confidence: 96%

“…Polysaccharides included in the second radiation are characterized by their chains of b-galactofuranose (Table 1). The character states in these orders are defined by the linkage types of the galactofuranose residues and the length of the chains (Leal et al, 1984(Leal et al, , 1992b(Leal et al, , 1993aGómez-Miranda et al, 1988Domenech et al, 1994Domenech et al, , 1999Prieto et al, 1995Prieto et al, , 2002Prieto et al, , 2004Ahrazem et al, 2001aAhrazem et al, , 2002bAhrazem et al, , 2007. This radiation includes the Diaporthales and Chaetosphaeriales in the Sordariomycetes, some Pleosporales and genera of uncertain position (i.e.…”

Section: Delimitation Of Suprageneric Ranksmentioning

confidence: 99%

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An assessment of fungal wall heteromannans as a phylogenetically informative character in ascomycetes

et al. 2010

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The fungal wall contains a small proportion of alkali-extractable water-soluble heteromannans (F1SS). They are the glycidic moiety of glycoproteins that have important roles in the biology of fungi. A considerable number of these polysaccharides has been described, differing in composition or linkage types. Their structure is similar in all species of a well-delimited genus, and teleomorphs and their corresponding anamorphs. Therefore, these polysaccharides have been used as chemotaxonomic markers at the genus level. Here we review cases where they have been found to resolve relationships around the genus level, and assess their phylogenetic informativeness in the delineation of taxa at family and higher ranks in the ascomycetes by comparison with molecular trees. Generally, the correlation is extremely good, from the species to the class level, though there are some divergences. In particular, comparisons suggest that the concept of the Sordariomycetes may eventually require revision as more molecular data become available. An analysis of the different chemical structures of these polysaccharides can lead to the proposal and testing of phylogenetic hypotheses, in a parallel manner to those generated from molecular trees. These molecules serve as an independent character similar to morphological or molecular characters.

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“…For example, in the reductive cleavage method, sugars are first permethylated and then the glucosidic bonds are cleaved with Et 3 SiH using Me 3 SiOSO 2 CF 3 as catalyst producing anhydroalditols. The latter compounds are further analyzed by GC-FID or GC-MS (Rolf and Gray 1982;Ahrazem et al 2001;Colquhoun et al 2001;Hashii et al 2003). NMR spectroscopy is a powerful tool in providing information on the linkages (α or β), geometry, and functional groups (-NH 2 , -OMe, -CH 3 , etc.)…”

Section: Panagiotopoulos and Sempérémentioning

confidence: 99%

Analytical methods for the determination of sugars in marine samples: A historical perspective and future directions

Panagiotopoulos

Sempéré

2005

Limnology & Ocean Methods

121

100

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Analytical techniques employed over the past three decades for sugar determination in marine samples are reviewed. This review first summarizes the different hydrolysis protocols used by marine biogeochemists to extract sugars from various marine matrices including sinking particulate organic matter (POM), dissolved organic matter (DOM), ultrafiltrated dissolved organic matter (UDOM), and sediments. The most commonly used methods for total sugar estimation are phenol sulfuric acid (PSA), 3‐methyl‐2‐benzo thiazoline hydrazone hydrochloride (MBTH), and 2,4,6‐tripyridyl‐s‐triazine (TPTZ). For individual sugars, gas chromatographyflame ionization detection (GC‐FID), liquid chromatography‐borate complexes (LC), high performance liquid chromatography‐ethylenediamine derivatives (HPLC‐EDA), high performance liquid chromatography‐p‐aminobenzoic acid derivatives (HPLC‐p‐AMBA), and high performance anion exchange chromatography‐pulsed amperometric detection (HPAEC‐PAD) have been employed, the last method being the most widely used. This study demonstrated that mild and strong hydrolysis give comparable results for open ocean samples, including POM, DOM, and UDOM (except for sediments) using chromatographic or colorimetric techniques. A survey of 130 sugar data sets revealed that more than half of the sugar‐C escapes analysis using chromatographic or colorimetric techniques compared to 1H and 13C NMR techniques. The reconciliation of the NMR and colorimetric or chromatographic measurements is likely to be the key to the understanding of the composition of DOM. This study also demonstrated that most of the published sugar data obtained by chromatographic techniques are related to suspended or sinking POM and sediments, and that there is a need for data on sugar composition in DOM and UDOM, which involve further analytical difficulties for chromatographic analysis.

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Chemical structure of a polysaccharide isolated from the cell wall of Arachniotus verruculosus and A. ruber

Cited by 14 publications

References 25 publications

Structural characterization of an α-1, 6-linked galactomannan from natural Cordyceps sinensis

Structural characterization of an α-1, 6-linked galactomannan from natural Cordyceps sinensis

An assessment of fungal wall heteromannans as a phylogenetically informative character in ascomycetes

Analytical methods for the determination of sugars in marine samples: A historical perspective and future directions

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